Acanthopanax senticosides B ameliorates oxidative damage induced by hydrogen peroxide in cultured neonatal rat cardiomyocytes

Liang, Qi-Ming; Yu, Xiaofeng; Qu, Su; Xu, Huali; Sui, Dayun

doi:10.1016/j.ejphar.2009.10.055

Cited by 22 publications

(15 citation statements)

References 28 publications

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“…The pretreatment of another saponin, acanthopanax senticoside B (200 μg/mL and 400 μg/mL) from Acanthopanax senticosus protects cardiomyocytes against H 2 O 2 induced oxidative damage by increasing the activity of catalase, GSH-Px, SOD, levels of reduced glutathione and increase in cell viability. 50 The saponins of I. cornuta exhibited protective effects against H 2 O 2 -mediated myocardial cell injury whereas did not show DPPH free radical scavenging activity (<30% at 200μM). 26 , 35 The antioxidant activity results suggested that the protective effects of saponins were not directly associated with their free radical scavenging effect and warrant to investigate further.…”

Section: Cardioprotective Activity Of Saponinsmentioning

confidence: 94%

Structural characteristics, bioavailability and cardioprotective potential of saponins

Singh

Chaudhuri

2018

Integrative Medicine Research

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Section: Cardioprotective Activity Of Saponinsmentioning

confidence: 94%

Structural characteristics, bioavailability and cardioprotective potential of saponins

Singh

Chaudhuri

2018

Integrative Medicine Research

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“…In the past decade, many components, such as glycosides, polysaccharides, flavonoids, amino acids and coumarins (Dong and Li, ), have been detected in A . senticosus , which is associated with many beneficial effects, such as reduction in fatigue (Huang et al ., ; Zhang et al ., ), oxygen free radicals (Liang et al ., ; Wang et al ., ), tumor formation (Shan et al ., ) and blood lipids (Cha et al ., ), and enhanced anticoagulant (Yang et al ., ), hypoglycemic (Cha et al ., ; Park et al ., ; Watanabe et al ., ) and immunoregulatory effects (Xie et al ., ; Han et al ., ). A .…”

Section: Introductionmentioning

confidence: 99%

Characterization of eleutheroside B metabolites derived from an extract of Acanthopanax senticosus Harms by high‐resolution liquid chromatography/quadrupole time‐of‐flight mass spectrometry and automated data analysis

Lü

Sun

Bai

et al. 2012

Biomedical Chromatography

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We elucidated the structure and metabolite profile of eleutheroside B, a component derived from the extract of Acanthopanax senticosus Harms, after oral administration of the extract in rats. Samples of rat plasma were collected and analyzed by selective high-resolution liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) automated data analysis method. A total of 11 metabolites were detected: four were identified, and three of those four are reported for the first time here. The three new plasma metabolites were identified on the basis of mass fragmentation patterns and literature reports. The major in vivo metabolic processes associated with eleutheroside B in A. senticosus include demethylation, acetylation, oxidation and glucuronidation after deglycosylation. A fairly comprehensive metabolic pathway was proposed for eleutheroside B. Our results provide a meaningful basis for drug discovery, design and clinical applications related to A. senticosus in traditional Chinese medicine.

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“…After myocardial ischemia-reperfusion, the body produces oxygen free radicals (OFR), and OFR-mediated cell membranes and subcellular membrane lipid peroxidation (LPO), while MDA is the LPO reaction product induced by OFR attacking the biomembrane. The amount of MDA reflects the degree of LPO, and is usually used to evaluate the degree of exposure to OFR [ 26 ]. In this study, after subjecting the cardiomyocytes to hypoxia/reoxygenation, the content of MDA in the medium increased significantly.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of acacetin isolated from Ziziphora clinopodioides Lam. (Xintahua) on neonatal rat cardiomyocytes

Yang

Liu

et al. 2014

Chin Med

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BackgroundThe total flavonoids from ethanol extract of the aerial part of Ziziphora clinopodioides Lam. (Lamlaceae) (Xintahua) showed protective activities against rat acute myocardial ischemia in rats. This study aims to isolate acacetin, a flavonoid, from the aerial part of Z. clinopodioides, to develop an HPLC method for its detection, and to evaluate its protective effects on neonatal rat cardiomyocytes.MethodsSephadex LH-20 silicagel and pillar layer chromatography silica gel were applied for the isolation and purification of acacetin and its structure was elucidated on the basis of 1H and 13C NMR spectroscopy. The content of acacetin in Z. clinopodioides collected from three different origins was determined by HPLC. The neonatal rat cardiomyocytes were isolated and cultured in vitro to establish a hypoxia/reoxygenation injury model. The viability of cardiomyocytes was measured by the MTT method. Changes of malondialdehyde (MDA) content in the medium were also determined.ResultsThe acacetin content in various batches of Z. clinopodioides ranged from 45.50 to 47.41 μg/g. Acacetin of 25, 10, 5 μg/mL significantly decreased the MDA content in a model of hypoxia/reoxygenation injury (P < 0.001, P < 0.001 and P = 0.033, respectively).ConclusionsAcacetin protects neonatal cardiomyocytes from the damage induced by hypoxia/reoxygenation stress through reduction of lipid peroxidation and enhancement of the antioxidant activity.

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Acanthopanax senticosides B ameliorates oxidative damage induced by hydrogen peroxide in cultured neonatal rat cardiomyocytes

Cited by 22 publications

References 28 publications

Structural characteristics, bioavailability and cardioprotective potential of saponins

Structural characteristics, bioavailability and cardioprotective potential of saponins

Characterization of eleutheroside B metabolites derived from an extract of Acanthopanax senticosus Harms by high‐resolution liquid chromatography/quadrupole time‐of‐flight mass spectrometry and automated data analysis

Protective effects of acacetin isolated from Ziziphora clinopodioides Lam. (Xintahua) on neonatal rat cardiomyocytes

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