ACADL Promotes the Differentiation of Goat Intramuscular Adipocytes

Abstract

Intramuscular fat (IMF) deposits help improve meat quality such as marbling, juicy, flavor and tenderness. Long-chain acyl-CoA dehydrogenase (ACADL) is a key enzyme for catalyzing fatty acid oxidation, and studies have shown ACADL is involved in the deposition and differentiation of intramuscular adipocytes. However, the effect of ACADL on intramuscular adipocytes differentiation in goats needs further study. In this study, to explore the mechanism of ACADL on the development of goat intramuscular adipocytes, we constructed an over-expression plasmids and a SI-RNA of ACADL to explore the function of ACADL on the development of goat IMF. It was found that overexpression of ACADL promoted the differentiation of goat intramuscular adipocytes, and promoted the expression of fat cell differentiation marker genes lipoprotein lipase (LPL), peroxisome proliferator activated receptor gamma (PPARγ), APETALA-2-like transcription factor gene (AP2), CCAT enhancer binding protein (CEBPα), preadipocyte Factor 1 (Pref-1) and CCAT enhancer binding protein (CEBPβ), and the opposite trend occurred after interference. In addition, we screened of this related tumor necrosis factor (TNF) signaling pathway by RNA-Seq. So, we validate the signaling pathway with inhibitor of TNF signaling pathway. In summary, these results indicate that ACADL promotes intramuscular adipocytes differentiation through activation TNF signaling pathway. This study provides an important basis for the mechanism of IMF development.

Transcriptomic analysis identifies the shared diagnostic biomarkers and immune relationship between Atherosclerosis and abdominal aortic aneurysm based on fatty acid metabolism gene set

“…A previous investigation presented genetic proof that mutations in PCBD1 can lead to the development of early-onset nonautoimmune diabetes, which exhibits characteristics resembling dominantly inherited HNF1A-diabetes ( Simaite et al, 2014 ). The activation of the TNF signaling pathway is facilitated by ACADL, leading to the promotion of intramuscular adipocyte differentiation ( Li et al, 2023 ). ACADL deficiency pronounced hypoglycemia, accumulation of lipids, elevated levels of free fatty acids in the bloodstream, and impaired insulin sensitivity in the liver ( Zhang et al, 2007 ).…”

Integrated 4D Analysis of Intramuscular Fat Deposition: Quantitative Proteomic and Transcriptomic Studies in Wannanhua Pig Longissimus Dorsi Muscle

“…It was found that ACADL, ACADM, and CPT2 were closely related, and all of them were at important PPI nodes. Long-chain acyl-CoA dehydrogenase (ACADL) is involved in the β-oxidation of long-chain fatty acids [ 30 , 31 ]. Medium-chain acyl-CoA dehydrogenase (ACADM) is involved in the β-oxidation process of medium-chain fatty acids and amino acids [ 32 ].…”

Investigation of seasonal changes in lipid synthesis and metabolism-related genes in the oviduct of Chinese brown frog (<em>Rana dybowskii</em>)

“… 22 Long-chain acyl-coenzyme A dehydrogenase (Acadl) is a critical enzyme in the initial step of fatty acid β-oxidation. 23 Peroxisomal β-oxidation involves the processing of various fatty acid substrates, acted upon by two or three distinct acyl-CoA oxidase (Acox) enzymes exhibiting varying specificities. 24 Eukaryotes also employ peroxisomal β-oxidation, catalyzed by acyl-CoA oxidase (Acox), instead of Acad proteins.…”