Acacia Xylan as a Substitute for Commercially Available Xylan and Its Application in the Production of Xylooligosaccharides

Sharma, Kananbala; Khaire, Kaustubh Chandrakant; Thakur, Abhijeet; Moholkar, Vijayanand S.

doi:10.1021/acsomega.0c00896

Cited by 34 publications

(19 citation statements)

References 51 publications

(124 reference statements)

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“…The greatest activity (5230 U/ml) was observed with beechwood xylan, which contains β-1,4 linkages between D-xylose residues 70 . The residual activity was 7.2% for pectin.…”

Section: Resultsmentioning

confidence: 99%

Paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production

Abdella

Ramadan

Hamouda

et al. 2021

Sci Rep

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Paecilomyces variotii xylanase was, produced in stirred tank bioreactor with yield of 760 U/mL and purified using 70% ammonium sulfate precipitation and ultra-filtration causing 3.29-fold purification with 34.47% activity recovery. The enzyme purity was analyzed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) confirming its monomeric nature as single band at 32 KDa. Zymography showed xylan hydrolysis activity at the same band. The purified enzyme had optimum activity at 60 °C and pH 5.0. The pH stability range was 5–9 and the temperature stability was up 70 °C. Fe2+and Fe3+ exhibited inhibition of xylanase enzyme while Cu2+, Ca2+, Mg2+ and Mn2+ stimulated its activity. Mercaptoethanol stimulated its activity; however, Na2-EDTA and SDS inhibited its activity. The purified xylanase could hydrolyze beechwood xylan but not carboxymethyl cellulose (CMC), avicel or soluble starch. Paecilomyces variotii xylanase Km and Vmax for beechwood were determined to be 3.33 mg/mL and 5555 U/mg, respectively. The produced xylanase enzyme applied on beech xylan resulted in different types of XOS. The antioxidant activity of xylo-oligosaccharides increased from 15.22 to 70.57% when the extract concentration was increased from 0.1 to 1.5 mg/mL. The enzyme characteristics and kinetic parameters indicated its high efficiency in the hydrolysis of xylan and its potential effectiveness in lignocellulosic hydrolysis and other industrial application. It also suggests the potential of xylanase enzyme for production of XOS from biomass which are useful in food and pharmaceutical industries.

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“…The greatest activity (5230 U/ml) was observed with beechwood xylan, which contains β-1,4 linkages between D-xylose residues 70 . The residual activity was 7.2% for pectin.…”

Section: Resultsmentioning

confidence: 99%

Paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production

Abdella

Ramadan

Hamouda

et al. 2021

Sci Rep

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“…However, the obtained results seem to be influenced by the demineralization procedure. Sharma et al 253 revealed that alkali lignin washed with hydrochloric and nitric acids produced more char and less condensable fraction than the raw sample. Conversely, alkali lignin samples washed with hydrofluoric acid produced a slightly lower char yield despite the reduction of the ash content.…”

Section: Effect Of Inherent Metals On Pyrolysis Processmentioning

confidence: 99%

Inherent Metal Elements in Biomass Pyrolysis: A Review

Giudicianni¹,

Gargiulo²,

Grottola³

et al. 2021

Energy Fuels

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One of the main drawbacks of using biomass as pyrolysis feedstock consists of the huge variability of the different biomass resources which undermines the viability of downstream processes. Inherent inorganic elements greatly contribute to enhance the compositional variability issues due to their catalytic effect (especially alkali and alkaline earth metals (AAEMs)) and the technical problems arising due to their presence. Due to the different pretreatments adopted in the experimental investigations as well as the different reactor configurations and experimental conditions, some mechanisms involving interactions between these elements and the biomass organic fraction during pyrolysis are still debated. This is the reason why predicting the results of these interactions by adapting the existing kinetic models of pyrolysis is still challenging. In this work, the most prominent experimental works of the last 10 years dealing with the catalytic effects of biomass inherent metals on the pyrolysis process are reviewed. Reaction pathways, products distributions and characteristics, and impacts on the products utilization are discussed with a focus on AAEMs and on potential toxic metallic elements in hyperaccumulator plants. The literature findings are discussed in relation to the applied laboratory procedures controlling the concentration of inherent inorganic elements, their capability of preserving the chemical integrity of the main organic components, and the ability of resembling the inherent inorganic elements in the raw biomass. The goal is to reveal possible experimental inconsistencies and to provide a clear scheme of the reaction pathways altered by the presence of inherent inorganics. This analysis paves the way for the examination of the proposed modifications of the existing models aiming at capturing the effect of inorganics on pyrolysis kinetics. Finally, the most relevant shortcomings and bottlenecks in existing experimental and modeling approaches are analyzed and directions for further studies are suggested.

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“…The hemicellulose of SB contains 55.2 (%, w/w) xylose and 10.7 (%, w/w) arabinose of the total hemicellulose content . The enzyme xylanase (endolytically) and xylosidase (exolytically) act on the main chain of xylan polysaccharides. − The use of accessory enzymes, such as α- l -arabinofuranosidase, to hydrolyse side chains synergistically acts and enhances the rate of overall hydrolysis and increases the production of monosaccharides . The monosaccharides, arabinose, and xylose act as antiglycemic agents and are of immense importance to the human health.…”

Section: Introductionmentioning

confidence: 99%

“…The monosaccharides, arabinose, and xylose act as antiglycemic agents and are of immense importance to the human health. These monosaccharides can be fermented in the food industry for the production of xylitol and arabitol or in fuel industries for enhanced bioethanol production. , The aim of this study was to facilitate the saccharification of the xylan portion of hemicellulose by including an additional enzyme, α- l -arabinofuranosidase, along with xylanase and xylosidase of sugarcane waste and achieve enhanced TRS yield by optimizing the various conditions of the process. The pretreatment conditions were optimized to reduce the hemicellulose loss during pretreatment.…”

Section: Introductionmentioning

confidence: 99%

Two-Step Saccharification of the Xylan Portion of Sugarcane Waste by Recombinant Xylanolytic Enzymes for Enhanced Xylose Production

et al. 2021

Self Cite

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Sugarcane bagasse (SB) and sugarcane trash (SCT) containing 30% hemicellulose content are the waste from the sugarcane industry. Hemicellulose being heterogeneous, more complex, and less abundant than cellulose remains less explored. The optimized conditions for the pretreatment of SB and SCT for maximizing the delignification are soaking in aqueous ammonia (SAA), 18.5 wt %, followed by heating at 70 °C for 14 h. The optimization of hydrolysis of SAA pretreated (ptd) SB and SCT by the Box–Behnken design in the first step of saccharification by xylanase ( Ct Xyn11A) and α- l -arabinofuranosidase ( Ps GH43_12) resulted in the total reducing sugar (TRS) yield of xylooligosaccharides (TRS (XOS) ) of 93.2 mg/g ptd SB and 85.1 mg/g ptd SCT, respectively. The second step of saccharification by xylosidase ( Bo GH43) gave the TRS yield of 164.7 mg/g ptd SB and 147.2 mg/g ptd SCT. The high-performance liquid chromatography analysis of hydrolysate obtained after the second step of saccharification showed 69.6% xylan-to-xylose conversion for SB and 64.1% for SCT. This study demonstrated the optimization of the pretreatment method and of the enzymatic saccharification by recombinant xylanolytic enzymes, resulting in the efficient saccharification of ptd hemicellulose to TRS by giving 73.5% conversion for SB and 71.1% for SCT. These optimized conditions for the pretreatment and saccharification of sugarcane waste can also be used at a large scale.

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Acacia Xylan as a Substitute for Commercially Available Xylan and Its Application in the Production of Xylooligosaccharides

Cited by 34 publications

References 51 publications

Paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production

Paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production

Inherent Metal Elements in Biomass Pyrolysis: A Review

Two-Step Saccharification of the Xylan Portion of Sugarcane Waste by Recombinant Xylanolytic Enzymes for Enhanced Xylose Production

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