Acacia Honey Modulates Cell Cycle Progression, Pro-inflammatory Cytokines and Calcium Ions Secretion in PC-3 Cell Lines

Muhammad, Aliyu; Odunola, Oyeronke A.; Farooq, Ahsana Dar; Mesaik, M. Ahmed; Choudhary, M. Iqbal; Fatima, Beenish; Qureshi, Tariq A; Erukainure, Ochuko L.

doi:10.4172/1948-5956.1000174

Cited by 21 publications

(13 citation statements)

References 40 publications

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“…Honey treatment of bladder cancer cell lines was shown to cause a considerable arrest of cell cycle in the sub-G 1 phase [68]. Recently, Aliyu and colleagues also showed that the cytotoxic effect of honey against non-small cell lung cancer cell (NCI-H460) was mediated via arrest of cell cycle at G 0 /G 1 phase [79]. Available data reveal that the ability of honey to arrest cell cycle is due to the various flavonoids and phenolic compounds in honey.…”

Section: Mechanisms Of the Antiproliferative Antimetastatic And Amentioning

confidence: 99%

Effects of Honey and Its Mechanisms of Action on the Development and Progression of Cancer

2014

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Honey is a natural product known for its varied biological or pharmacological activities-ranging from anti-inflammatory, antioxidant, antibacterial, antihypertensive to hypoglycemic effects. This review article focuses on the role of honey in modulating the development and progression of tumors or cancers. It reviews available evidence (some of which is very recent) with regards to the antimetastatic, antiproliferative and anticancer effects of honey in various forms of cancer. These effects of honey have been thoroughly investigated in certain cancers such as breast, liver and colorectal cancer cell lines. In contrast, limited but promising data are available for other forms of cancers including prostate, bladder, endometrial, kidney, skin, cervical, oral and bone cancer cells. The article also underscores the various possible mechanisms by which honey may inhibit growth and proliferation of tumors or cancers. These include regulation of cell cycle, activation of mitochondrial pathway, induction of mitochondrial outer membrane permeabilization, induction of apoptosis, modulation of oxidative stress, amelioration of inflammation, modulation of insulin signaling and inhibition of angiogenesis. Honey is highly cytotoxic against tumor or cancer cells while it is non-cytotoxic to normal cells. The data indicate that honey can inhibit carcinogenesis by modulating the molecular processes of initiation, promotion, and progression stages. Thus, it may serve as a potential and promising anticancer agent which warrants further experimental and clinical studies.

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Section: Mechanisms Of the Antiproliferative Antimetastatic And Amentioning

confidence: 99%

Effects of Honey and Its Mechanisms of Action on the Development and Progression of Cancer

2014

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“…Cytokine activity is influenced by the microenvironment in which they are produced and as such may have pro-(Th1) or anti-inflammatory (Th2) actions [12]. Tumor necrosis factor (TNF-α), an inflammatory cytokine that is highly expressed in breast and cervical carcinomas play an important role in the regulation of both induction and protection in breast and cervical cancer [13,14], and apoptosis [15].…”

Section: Introductionmentioning

confidence: 99%

Anti-proliferative and immunomodulatory activities of fractions from methanol root extract of Abrus precatorius L

et al. 2019

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Background: Abrus precatorius possesses various therapeutic properties including anticancer potentials. This study evaluated the anti-proliferative activities of fractions of methanol root extract of A. precatorius on breast and cervical cancer cells and their immunomodulatory effect. Phytochemical screening was done by FTIR and GCMS. In vitro anti-proliferative effect was evaluated on human breast cancer (AU565) and cervical cancer (HeLa) cells and on murine fibroblast (NIH 3 T3) cells. Antioxidant activity was performed via DPPH radical scavenging assay. The immunomodulatory potential of fractions was evaluated by inhibition of phagocytes oxidative burst (ROS), Nitric oxide (NO) and proinflammatory cytokine TNF-α. Results: A. precatorius fractions showed different chemical groups and were somewhat selective in antiproliferative activity against studied cancer cells. Ethyl acetate fraction showed the most significant antiproliferative activity with IC 50 values of 18.10 μg/mL and 11.89 μg/mL against AU565 and HeLa cells respectively. Hexane fraction significantly (p < 0.05) inhibited HeLa cells (IC 50 18.24 ± 0.16 μg/mL), whereas aqueous fraction showed mild inhibition (IC 50 46.46 ± 0.14 μg/mL) on AU565 cell proliferation. All fractions showed no cytotoxicity against NIH-3 T3 murine fibroblast normal cells. All fractions showed potent and significant (p < 0.001) DPPH radical scavenging activity as well as suppressed phagocytic oxidative burst. Hexane (< 1 μg/mL), ethyl acetate (< 1 μg/mL), and butanol (5.74 μg/ mL) fractions potently inhibited the cytokine TNF-α, hexane (< 1 μg/mL) and ethyl acetate (< 1 μg/mL) fractions also potently inhibited NO. Conclusions: The antiproliferative activities and suppressive effect on the phagocytic oxidative burst, NO and proinflammatory cytokine might be due to the synergistic actions of bioactive compounds especially flavonoids present in the assayed fractions and therefore, suggest chemotherapeutic use of A. precatorius in cancer treatment.

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“…Bioactive compounds such as (+)-catechin, (−)-epicatechin, (−)-epicatechin-3- O -gallate, epigallocatechin-3- O -gallate, quercetin, and (+)-cyanidanol have been isolated from different Acacia species 15 . Compounds identified from Acacia species are known to modulate various signaling pathways in breast and prostate cancer 18 19 . However, these studies have generally utilized the total extracts from various catechin sources reflecting effects of combined activities of several compounds rather than of a single compound.…”

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confidence: 99%

Growth inhibition and apoptosis in cancer cells induced by polyphenolic compounds of Acacia hydaspica: Involvement of multiple signal transduction pathways

Afsar

Trembley

Salomon

et al. 2016

Sci Rep

102

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Acacia hydaspica R. Parker is known for its medicinal uses in multiple ailments. In this study, we performed bioassay-guided fractionation of cytotoxic compounds from A. hydaspica and investigated their effects on growth and signaling activity in prostate and breast cancer cell lines. Four active polyphenolic compounds were identified as 7-O-galloyl catechin (GC), catechin (C), methyl gallate (MG), and catechin-3-O-gallate (CG). The four compounds inhibited prostate cancer PC-3 cell growth in a dose-dependent manner, whereas CG and MG inhibited breast cancer MDA-MB-231 cell growth. All tested compounds inhibited cell survival and colony growth in both cell lines, and there was evidence of chromatin condensation, cell shrinkage and apoptotic bodies. Further, acridine orange, ethidium bromide, propidium iodide and DAPI staining demonstrated that cell death occurred partly via apoptosis in both PC-3 and MDA-MB-231 cells. In PC-3 cells treatment repressed the expression of anti-apoptotic molecules Bcl-2, Bcl-xL and survivin, coupled with down-regulation of signaling pathways AKT, NFκB, ERK1/2 and JAK/STAT. In MDA-MB-231 cells, treatment induced reduction of CK2α, Bcl-xL, survivin and xIAP protein expression along with suppression of NFκB, JAK/STAT and PI3K pathways. Our findings suggest that certain polyphenolic compounds derived from A. hydaspica may be promising chemopreventive/therapeutic candidates against cancer.

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Acacia Honey Modulates Cell Cycle Progression, Pro-inflammatory Cytokines and Calcium Ions Secretion in PC-3 Cell Lines

Cited by 21 publications

References 40 publications

Effects of Honey and Its Mechanisms of Action on the Development and Progression of Cancer

Effects of Honey and Its Mechanisms of Action on the Development and Progression of Cancer

Anti-proliferative and immunomodulatory activities of fractions from methanol root extract of Abrus precatorius L

Growth inhibition and apoptosis in cancer cells induced by polyphenolic compounds of Acacia hydaspica: Involvement of multiple signal transduction pathways

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