Acacia honey and chrysin reduce proliferation of melanoma cells through alterations in cell cycle progression

Abstract

Abstract. Honey has long been used in medicine for different purposes. Only recently, however, its antioxidant property and preventive effects against different diseases, such as cancer, have been highlighted. Chrysin (5,7-dihydroxyflavone) is a natural flavone commonly found in acacia honey. It has previously been shown to be an anti-tumor agent. In this study, we investigated the antiproliferative role of honey or chrysin on human (A375) and murine (B16-F1) melanoma cell lines. The results of the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and the trypan blue exclusion test showed that both the tested compounds were able to induce an antiproliferative effect on melanoma cells in a dose-and time-dependent manner. Flow cytometry analysis indicated that cytotoxicity induced by honey or chrysin was mediated by G 0 /G 1 cell cycle arrest and induction of hyperploid progression. Our results suggest that the anti-proliferative effects of honey are due mainly to the presence of chrysin. Chrysin may therefore be considered a potential candidate for both cancer prevention and treatment. Further investigation is needed to validate the contribution of chrysin in tumor therapy in vivo.

Topical Nanoemulgel for the Treatment of Skin Cancer: Proof-of-Technology

“…Chrysin disrupts the homeostasis of mitochondria and endoplasmic reticulum E in apoptosis induction and also activates programmed cell death, or autophagy. The antiproliferative effect of chrysin has been reported in various cancer cell lines including melanoma cell lines, namely human A375 and A375.S2 and murine B16-F1 [ 28 , 29 ], A431 human epidermoid carcinoma cells [ 30 ], and the CT26 colon cancer cell line [ 28 , 31 ] have shown that chrysin inhibits MMP-2 activity and at concentrations lower than the lethal concentration, chrysin inhibits cell mobility, migration, and the invasion of cancer cells when analyzed using wound healing and Transwell filter assay. A recent review on the future perspectives of the broad-spectrum preclinical antitumor activity of chrysin has emphasized the need to formulate chrysin nanoparticles to overcome the potential drawback of its poor bioavailability [ 32 ].…”

Uses of Natural Honey in Cancer: An Updated Review

“…Their results showed that honey could affect the metabolic activity of both studied cells in a dose-and time-dependent manner. 33 Antitumor properties of MKH on melanoma were also investigated by Cabezudo et al in a mouse model. Based on their results, MKH could inhibit the growth of B16-F melanoma cells in a time-and dose-dependent fashion.…”

“…32 Acacia honey (ACH) produced by Apis mellifera fed with acacia flowers has been reported to be able to cause an antiproliferative effect on melanoma cells. 33 In addition, the preventive effects of individual active compounds extracted from honey on cancer have been demonstrated. 34…”

Study of anticancer, antimicrobial, immunomodulatory, and silver nanoparticles production by Sidr honey from three different sources

“…Others showed that 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay method confirmed that antiproliferative effect of honey is in a dose-and time-dependent manner (Pichichero et al, 2010). Honey or its components mediate inhibition of cell growth due to its perturbation of the cell cycle (Oršolić et al, 2010;Pichichero et al, 2010).…”