Acacetin from Traditionally Used Saussurea involucrata Kar. et Kir. Suppressed Adipogenesis in 3T3-L1 Adipocytes and Attenuated Lipid Accumulation in Obese Mice

Liou, Chian-Jiun; Wu, Sibin; Chen, Li‐Chen; Yeh, Kuo‐Wei; Chen, Chih‐Ying; Huang, Wen‐Chung

doi:10.3389/fphar.2017.00589

Cited by 48 publications

(39 citation statements)

References 50 publications

(70 reference statements)

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“…Interestingly, acacetin-treated mice had a higher body weight than that of in the control group, a finding in stark contrast to that of Liou et al's, showing that in high fat diet fed-obese mice, acacetin significantly reduced body weight [43]. Burke et al recently also reported that citrus flavonoids supplementation to a high fat, cholesterol-containing diet protected against obesity.…”

Section: Discussionmentioning

confidence: 88%

Acacetin exert antioxidant potential against atherosclerosis through Nrf2 pathway in apoE-/- mice

Song

et al. 2020

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Background : Oxidative stress has a considerable influence on endothelial cell dysfunction and atherosclerosis. Acacetin, an anti-inflammatory and antiarrhythmic, is frequently used in the treatment of myocarditis, albeit its role in managing atherosclerosis is currently unclear. Thus, we evaluated the regulatory effects of acacetin in maintaining endothelial cell function and further investigated whether the flavonoid could attenuate atherosclerosis in apolipoprotein E deficiency (apoE -/- ) mice. Methods : Different concentrations of acacetin were tested on EA.hy926 cells, either induced or non-induced by human oxidized low density lipoprotein (oxLDL), to clarify its influence on cell viability, cellular reactive oxidative stress (ROS) level, apoptotic ratios and other regulatory effects. In vivo, apoE -/- mice were fed either a western diet or a chow diet. Acacetin prodrug (15mg/kg) was injected subcutaneously two times a day for 12weeks. The effects of acacetin on the atherosclerotic process, plasma inflammatory factors and lipid metabolism were also investigated. Results : Acacetin significantly increased EA.hy926 cell viability by reducing the ratios of apoptotic and necrotic cells at 3μM. Moreover, 3μM Acacetin clearly decreased ROS levels and enhanced reductase protein expression through MsrA and Nrf2 pathway through phosphorylation of Nrf2 and degradation of Keap1. In vivo , acacetin treatment remarkably attenuated atherosclerosis by increasing reductase levels in circulation and aortic roots, decreasing plasma inflammatory factor levels as well as accelerating lipid metabolism in Western diet-fed apoE -/- mice. Conclusions : Our findings demonstrate the anti-oxidative and anti-atherosclerotic effects of acacetin, in turn suggesting its potential therapeutic value in atherosclerotic-related cardiovascular diseases (CVD).

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Section: Discussionmentioning

confidence: 88%

Acacetin exert antioxidant potential against atherosclerosis through Nrf2 pathway in apoE-/- mice

Song

et al. 2020

Preprint

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“…In addition, acacetin reduced the transcript and protein expressions of adipogenesis-related transcription factors, including the CCAAT/enhancer-binding protein in cultured adipocytes. In parallel, acacetin treatment increased sirtuin 1 expression and AMPK phosphorylation (Liou et al, 2017). Besides, acacetin was able to suppress the levels of inflammatory mediators, as well as levels of MAPK and NF-kB pathways, in cultured macrophages, as treated with differentiated media deriving from cultured 3T3-L1 adipocytes (Su et al, 2014a;Liou et al, 2017).…”

Section: Adipogenesis Suppressionmentioning

confidence: 97%

“…In parallel, acacetin treatment increased sirtuin 1 expression and AMPK phosphorylation (Liou et al, 2017). Besides, acacetin was able to suppress the levels of inflammatory mediators, as well as levels of MAPK and NF-kB pathways, in cultured macrophages, as treated with differentiated media deriving from cultured 3T3-L1 adipocytes (Su et al, 2014a;Liou et al, 2017). In insulin-resistant adipocytes, acacetin treatment significantly reduced the levels of chemokines MCP-1 and CCL5 in these cells, as to restore the resistance (Liou et al, 2017).…”

Section: Adipogenesis Suppressionmentioning

confidence: 97%

Saussureae Involucratae Herba (Snow Lotus): Review of Chemical Compositions and Pharmacological Properties

et al. 2020

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Saussureae Involucratae Herba is the dried ground part of Saussurea involucrata (Kar. et Kir.) Sch.-Bip, which is also named as "Snow lotus" and being used in traditional Uyghur and/or Chinese medicine. This rare herb can be found at 4,000 m elevation in western part of Tianshan Mountain, Xinjiang China. According to China Pharmacopoeia (2015), the major pharmaceutical values of "Snow lotus" (Xuě liánhuā in Chinese) are alleviating rheumatoid arthritis, accelerating blood circulation and mitigating other "cold" syndromes. Traditionally, the clinical application of "Snow lotus" includes the treatments in inflammation-associated disorder, blood circulation acceleration and heat and dampness elimination. Recent studies suggested that "Snow lotus" possessed therapeutic effects associating with anti-cancer, anti-oxidation, adipogenesis suppression and neuroprotection activities, which were proposed to be related with its bioactive constitutes, i.e. acacetin, hispidulin, and rutin. In the present review, we aim to summarize pharmacological effects and underlying cell signaling pathways of "Snow lotus" in treating various medical problems.

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“…As a flavonoid,Liou CJ et al reported that acacetin can reduce the expression of transcription factors related to adipogenesis, inhibit lipid accumulation in adipocytes, and inhibit the inflammatory response of macrophages stimulated by 3T3-L1 cell differentiation medium [40] ,while Wu WY et al show that it can participate in anti-oxidation, anti-inflammatory and anti-apoptosis by activating AMPK-mediated Nrf2 pathway, which will play its protective effect on myocardial cell hypoxia / reoxygenation injury [41] .Based on the above research status, we speculate that the above chemical components are most likely related medicinal substances of HLJDD against atherosclerosis.…”

Section: Figure7hljdd-key Active Ingredients-key Targets-pathway Netmentioning

confidence: 99%

Network Pharmacological Analysis of Huanglian Jiedu Decoction for Anti- Atherosclerosis

Wu¹,

Jiang²,

Huang³

et al. 2020

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Objective: We aimed to predict the possible active components,key targets and pathways of Huanglian Jiedu Decoction(HLJDD) for anti-atherosclerosis. Methods: The TCMSP database was searched to obtain the active components and targets of HLJDD, the GeneCards and OMIM databases were searched to obtain related targets of atherosclerosis, and we obtain the intersection targets of them, which are the potential targets of HLJDD for anti-atherosclerosis.Application of Cytoscape 3.6.0 software to build a herbal-active ingredient-potential target regulation network.We perform protein-protein interaction(PPI) network analysis of potential targets through STRING 11.0 database and obtain the key targets,and the results of PPI network of key targets were visualized by Cytoscape3.6.0 software. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the key targets were performed using STRING11.0 database, and we finally constructed the possible pharmacological network of HLJDD for anti-atherosclerosis .Results: We finally obtained 14 key active ingredients of HLJDD, 65 key targets of anti-atherosclerosis, and 14 key active ingredients corresponded to 52 of these targets. These targets are mainly involved in biological processes such as reaction to organic substance, reaction to chemical stimulation,etc.They mainly involved in biological signaling pathways such as pathways in cancer,IL-17 signaling pathway,etc. Conclusion: HLJDD may act on 52 key targets such as PTGS2, HSP90AA1 and RELA through 14 key active ingredients, and influence the signaling pathways including fluid shear stress and atherosclerosis,PI3K-Akt signaling pathway,IL-17 signaling pathway,AGE-RAGE signaling pathway in diabetic complications,TNF signaling pathway,etc.Thus, it may play an anti-atherosclerosis role by inhibiting inflammatory reaction, oxidative stress and improving hemodynamics,etc.

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Acacetin from Traditionally Used Saussurea involucrata Kar. et Kir. Suppressed Adipogenesis in 3T3-L1 Adipocytes and Attenuated Lipid Accumulation in Obese Mice

Cited by 48 publications

References 50 publications

Acacetin exert antioxidant potential against atherosclerosis through Nrf2 pathway in apoE-/- mice

Acacetin exert antioxidant potential against atherosclerosis through Nrf2 pathway in apoE-/- mice

Saussureae Involucratae Herba (Snow Lotus): Review of Chemical Compositions and Pharmacological Properties

Network Pharmacological Analysis of Huanglian Jiedu Decoction for Anti- Atherosclerosis

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