AC5 Surgical Hemostat™ as an effective hemostatic agent in an anticoagulated rat liver punch biopsy model

Csukás, Domokos; Urbanics, Rudolf; Moritz, Annie; Ellis-Behnke, Rutledge

doi:10.1016/j.nano.2015.01.001

Cited by 21 publications

(26 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Current approaches are the use of biologically derived adhesives, which synthesized using polypeptides by the self‐assembly method or modified chitosan (CTS) with varying successes . These materials have several problems, such as batch‐to‐batch variability and carry risk of biological contamination, because of xeno‐/allogenic characteristics . For example, Kumar et al reported that snake venom laded self‐assembling nanofibrous peptide hydrogel is a facile method for surgical hemostasis .…”

Section: Introductionmentioning

confidence: 99%

Active nano/microbilayer hemostatic agents for diabetic rat bleeding model

et al. 2016

View full text Add to dashboard Cite

Patients with diabetes mellitus have an increased cardiovascular risk due to the abnormality of hemostatic system components. Therefore, hemostasis is an important concept when considering that diabetics are under risk due to potential bleeding complications during surgical operation. The aim of our study was to examine the efficiency of a fabricated nano/microbilayer hemostatic dressing for bleeding control in diabetic patients. For this purpose, we prepared a nano/microbilayer hemostatic dressing that has a porous sublayer, including chitosan (CTS), bacterial cellulose (BC) as basement and active agents in coagulation cascade, such as vitamin K (Vit K), protamine sulfate (PS), and kaolin (Kao) as a filler and an upper layer consisting of silk fibroin (SF) or SF/phosphatidylcholine (PC) blend to achieve complete hemostasis in diabetic rats. Coagulative performances of the prepared hemostatic dressings were examined by the determination of bleeding time, blood loss, and mortality rate through diabetic rat femoral artery injury model. The percent of diabetic rat blood absorption was found to be 247 ± 5% for gauze as opposed to 2214 ± 56% for SF-coated PS/BC/CTS. Vit K-reinforced within 138 s and SF-coated BC/CTS hemostatic dressings within 144 s showed a rapid coagulation time. In vivo coagulation studies demonstrated that hemostatic agent-reinforced BC/CTS hemostatic dressing, especially PS/BC/CTS showed a significant hemostatic plug formation. This study suggests that the high positive charge and porosity give to these hemostatic agents reinforced hemostatic dressings the ability to rapidly swell and to promote the accumulation of red blood cells and platelets through electrostatic interactions. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1573-1585, 2017.

show abstract

Section: Introductionmentioning

confidence: 99%

Active nano/microbilayer hemostatic agents for diabetic rat bleeding model

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Importantly, under intermittent high shear rates, peptide hydrogels recover to preshear G′ values within 60 s. Together these results suggest the ability to use a material, which is easily injectable, and that can reself-assemble to form a strong conformal gel, presenting a physical barrier to bleeding. 6–8,51 …”

Section: Resultsmentioning

confidence: 99%

“…4,5 The critical design requirement for hemostats are to (1) provide a localized conformal physical barrier to entrap red blood cells (RBC), (2) provide a chemical platform that accelerates clotting regardless of the presence of heparin, (3) stop bleeding in a rapid fashion (<1 min), and (4) prevent hemorrhaging during subsequent manipulation. 6–9 Classic approaches for controlling intraoperative and perioperative bleeding involve use of physical pressure and sutures. 2,6,10 Sutureless techniques typically involve foams, sealants, and adhesives; synthetic hemostats are typically made of cyanoacrylates, polyurethanes, and polyethylene glycol.…”

Section: Introductionmentioning

confidence: 99%

“…6–9 Classic approaches for controlling intraoperative and perioperative bleeding involve use of physical pressure and sutures. 2,6,10 Sutureless techniques typically involve foams, sealants, and adhesives; synthetic hemostats are typically made of cyanoacrylates, polyurethanes, and polyethylene glycol. 2,9,11,12 These hemostats can result in toxic byproducts of polymerization/degradation, solvents, and immediate tissue necrosis in addition to local irritation and inflammation.…”

Section: Introductionmentioning

confidence: 99%

“…These materials suffer from batch-to-batch variability, are xeno-/allo-genic and therefore carry risk of latent biologic contamination. 6 Commercially available GelFoam is a porcine derived lyophilized collagen substrate to physically absorb and block bleeding. In addition it has domains in the collagen which can modulate binding to vWF (von Willebrand Factor), platelets, macrophages, and subsequent activation of intrinsic and extrinsic coagulation pathways.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nanofibrous Snake Venom Hemostat

Kumar

Wickremasinghe

Shi

et al. 2015

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Controlling perioperative bleeding is of critical importance to minimize hemorrhaging and fatality. Patients on anticoagulant therapy such as heparin have diminished clotting potential and are at risk for hemorrhaging. Here we describe a self-assembling nanofibrous peptide hydrogel (termed SLac) that on its own can act as a physical barrier to blood loss. SLac was loaded with snake-venom derived Batroxobin (50 μg/mL) yielding a drug-loaded hydrogel (SB50). SB50 was potentiated to enhance clotting even in the presence of heparin. In vitro evaluation of fibrin and whole blood clotting helped identify appropriate concentrations for hemostasis in vivo. Batroxobin-loaded hydrogels rapidly (within 20s) stop bleeding in both normal and heparin-treated rats in a lateral liver incision model. Compared to standard of care, Gelfoam, and investigational hemostats such as Puramatrix, only SB50 showed rapid liver incision hemostasis post surgical application. This snake venom-loaded peptide hydrogel can be applied via syringe and conforms to the wound site resulting in hemostasis. This demonstrates a facile method for surgical hemostasis even in the presence of anticoagulant therapies.

show abstract

Progress Toward the Clinical Translation of Bioinspired Peptide and Protein Assemblies

Hainline

Fries

Collier

2017

Adv Healthcare Materials

View full text Add to dashboard Cite

Supramolecular materials composed of proteins and peptides have been receiving considerable attention toward a range of diseases and conditions from vaccines to drug delivery. Owing to the relative newness of this class of materials, the bulk of work to date has been preclinical. However, examples of approved treatments particularly in vaccines, dentistry, and hemostasis demonstrate the translational potential of supramolecular polypeptides. Critical milestones in the clinical development of this class of materials and currently approved supramolecular polypeptide therapies are described in this study. Additional examples of not-yet-approved materials that are steadily advancing toward clinical use are also featured. Spherical assemblies such as virus-like particles, designed protein nanoparticles, and spherical peptide amphiphiles are highlighted, followed by fiber-forming systems such as fibrillizing peptides, fiber-forming peptide-amphiphiles, and filamentous bacteriophages.

show abstract

AC5 Surgical Hemostat™ as an effective hemostatic agent in an anticoagulated rat liver punch biopsy model

Cited by 21 publications

References 15 publications

Active nano/microbilayer hemostatic agents for diabetic rat bleeding model

Active nano/microbilayer hemostatic agents for diabetic rat bleeding model

Nanofibrous Snake Venom Hemostat

Progress Toward the Clinical Translation of Bioinspired Peptide and Protein Assemblies

Contact Info

Product

Resources

About