Ac34FGlcNAz is an effective metabolic chemical reporter for O-GlcNAcylated proteins with decreased S-glyco-modification

Longer fatty acid-protected GalNAz enables efficient labeling of proteins in living cells with minimized S-glyco modification

“…Meanwhile, our group also disclosed several probes for specific labeling of intracellular O -GlcNAcylated proteins without this leading to S -glyco modification, including Ac 3 6deoGlcNAz 17 and Ac 3 4FGlcNAz. 18 Our findings indicated that subtle modifications of the structure of GlcNAc will, surprisingly, avoid this S -linked side reaction. Encouraged by these results, we sought to confirm the roles of different protected groups in processes for the occurrence of S -glyco modification.…”