Ac2PIM-responsive miR-150 and miR-143 Target Receptor-interacting Protein Kinase 2 and Transforming Growth Factor Beta-activated Kinase 1 to Suppress NOD2-induced Immunomodulators

Prakhar, Praveen; Holla, Sahana; Ghorpade, Devram Sampat; Gilleron, Martine; Puzo, Germain; Udupa, Vibha; Balaji, Kithiganahalli Narayanaswamy

doi:10.1074/jbc.m115.662817

Cited by 12 publications

(8 citation statements)

References 71 publications

(73 reference statements)

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“…S3 . The MAPK/ERK pathway is intimately linked to the altered miRNAs seen in nodular SGC and it is possible that these miRNAs are acting as tumour suppressors to inhibit this pathway 26 , 27 . Moreover, synthetic miR-143 has been shown to silence KRAS signalling including its effector signalling molecules AKT and ERK (MAPK1).…”

Section: Resultsmentioning

confidence: 99%

MicroRNA and transcriptome analysis in periocular Sebaceous Gland Carcinoma

Bladen

Wang

Sangaralingam

et al. 2018

Sci Rep

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Sebaceous gland carcinoma (SGC) is a rare, but life-threatening condition with a predilection for the periocular region. Eyelid SGC can be broadly categorised into two subtypes, namely either nodular or pagetoid with the latter being more aggressive and requiring radical excision to save life. We have identified key altered microRNAs (miRNA) involved in SGC shared by both subtypes, hsa-miR-34a-5p and hsa-miR-16-5p. However, their gene targets BCL2 and MYC were differentially expressed with both overexpressed in pagetoid but unchanged in nodular suggesting different modes of action of these two miRNAs on BCL/MYC expression. Hsa-miR-150p is nodular-specifically overexpressed, and its target ZEB1 was significantly downregulated in nodular SGC suggesting a tumour suppressor role. Invasive pagetoid subtype demonstrated specific overexpression of hsa-miR-205 and downregulation of hsa-miR-199a. Correspondingly, miRNA gene targets, EZH2 (by hsa-miR-205) and CD44 (by hsa-miR-199a), were both overexpressed in pagetoid SGC. CD44 has been identified as a potential cancer stem cell marker in head and neck squamous cell carcinoma and its overexpression in pagetoid cells represents a novel treatment target. Aberrant miRNAs and their gene targets have been identified in both SGC subtypes, paving the way for better molecular understanding of these tumours and identifying new treatment targets.

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Section: Resultsmentioning

confidence: 99%

MicroRNA and transcriptome analysis in periocular Sebaceous Gland Carcinoma

Bladen

Wang

Sangaralingam

et al. 2018

Sci Rep

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“…Furthermore, the P300 protein may activate transcription by binding to transcription factors; thus, P300 is also known as a transcriptional coacti-vator ( 34 – 36 ). Previous studies have demonstrated that miR-150 regulates the expression of the transcriptional co-activator P300 ( 37 , 38 ). Accordingly, the present study identified that P300 was a downstream target of MIAT/miR-150-5p.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA myocardial infarction‑associated transcript is associated with the microRNA‑150‑5p/P300 pathway in cardiac hypertrophy

et al. 2018

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In numerous diseases, abnormal expression of myocardial infarction-associated transcript (MIAT) has been reported to be involved in cell proliferation, apoptosis and migration. However, whether this long non-coding RNA MIAT has a regulatory effect on heart hypertrophy requires further investigation. To this end, the present study evaluated MIAT in hypertrophic cardiomyocytes in vitro and in vivo. Neonatal rat ventricular myocytes (NRVMs) were induced by isoproterenol (ISO) to create a cell hypertrophy model, and mice were intraperitoneally injected with ISO to establish an animal model. Echocardiography, immunofluorescence staining, western blot analysis, RNA isolation and reverse transcription-polymerase chain reaction were applied to test the involvement of MIAT in cardiac hypertrophy. The results revealed that MIAT was upregulated under ISO stimulation at the mRNA level both in vivo and in vitro. Silencing of MIAT resulted in decreased expression levels of atrial natriuretic peptide and brain natriuretic peptide in ISO-treated NRVM cardiomyocytes, confirming the connection between MIAT and hypertrophy. Furthermore, MIAT small interfering RNA significantly increased microRNA (miR)-150 and decreased P300 expression in NRVMs. In conclusion, the MIAT/miR-150-5p axis targets P300 as a positive regulator of cardiomyocyte hypertrophy.

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“…and transforming growth factor β-activated kinase 1 (TAK1), which are the mediators of the NOD2 signaling pathway, and applying their inhibitory effect on the inflammation process (Prakhar et al, 2015). are the other miRNAs that are associated with NOD2 and are involved in the pathogenesis of IBD, especially Crohn's disease (Brain et al, 2013;Chuang, Chuang, Zhai, Wu, & Kwon, 2013;Ghorpade, Sinha, Holla, Singh, & Balaji, 2013).…”

Section: Nucleotide Oligomerization Domains Toll-like Receptors and Mmentioning

confidence: 99%

MiRNAs and inflammatory bowel disease: An interesting new story

Moein

Vaghari‐Tabari

Qujeq

et al. 2018

Journal Cellular Physiology

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Inflammatory bowel disease (IBD), as a chronic and recurrent inflammatory disorder, is caused by a dysregulated and aberrant immune response to exposed environmental factors in genetically susceptible individuals. Despite huge efforts in determining the molecular pathogenesis of IBD, an increasing worldwide incidence of IBD has been reported. MicroRNAs (miRNAs) are a set of noncoding RNA molecules that are about 22 nucleotides long, and these molecules are involved in the regulation of the gene expression. By clarifying the important role of miRNAs in a number of diseases, their role was also considered in IBD; numerous studies have been performed on this topic.In this review, we attempt to summarize a number of studies and discuss some of the recent developments in the roles of miRNAs in the pathophysiology, diagnosis, and treatment of IBD.

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Ac2PIM-responsive miR-150 and miR-143 Target Receptor-interacting Protein Kinase 2 and Transforming Growth Factor Beta-activated Kinase 1 to Suppress NOD2-induced Immunomodulators

Cited by 12 publications

References 71 publications

MicroRNA and transcriptome analysis in periocular Sebaceous Gland Carcinoma

MicroRNA and transcriptome analysis in periocular Sebaceous Gland Carcinoma

Long noncoding RNA myocardial infarction‑associated transcript is associated with the microRNA‑150‑5p/P300 pathway in cardiac hypertrophy

MiRNAs and inflammatory bowel disease: An interesting new story

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