The operating room (OR) can be a high-stress area for healthcare workers. A great deal of expertise, teamwork, and precision is needed for the area to run smoothly and achieve desired patient outcomes. Incivility in healthcare has been recognized in the literature as a cause of healthcare worker distraction with increased risk of medical errors, reduce collegiality, and employee turnover. A survey was sent to nurses who work in operating rooms in a southern state. Forty-three nurses responded to the survey. It was found that most had experienced incivility in the workplace (85%). Fiftyeight percent stated that they had dreaded going to work due to workplace incivility. Twenty-eight percent said that they had experienced stress-related illnesses due to workplace incivility. Physicians and fellow nurses were found to be the most common perpetrators of uncivil behaviors in the OR, compared with supervisors, patients, or other hospital employees. The study's findings underscored the need for hospitals and healthcare systems to focus on reducing incivility the operating room.