Abundantly expressed hepatic genes and their differential expression in liver of prelaying and laying geese

Yen, Chih-Feng; Lin, Emily; Wang, Y. H.; Wang, P. H.; Lin, Hoang‐Yan; Hsu, Jenn-Chung; Wu, Leang‐Shin; Yu, Jian; Ding, Shih‐Torng

doi:10.3382/ps.2008-00473

Cited by 11 publications

(10 citation statements)

References 57 publications

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“…It is secreted into the blood circulation and is able to bind the various forms of vitamin D [131]. It is at higher levels in geese during the laying than pre-laying period, indicative of roles in lipid metabolism related to egg formation [132]. In humans, VDBP is implicated in cancer and coronary artery disease [133,134].…”

Section: Discussionmentioning

confidence: 99%

Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds

Phillips

Kraev

Lange

2020

Biology

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Pelagic seabirds are amongst the most threatened of all avian groups. They face a range of immunological challenges which seem destined to increase due to environmental changes in their breeding and foraging habitats, affecting prey resources and exposure to pollution and pathogens. Therefore, the identification of biomarkers for the assessment of their health status is of considerable importance. Peptidylarginine deiminases (PADs) post-translationally convert arginine into citrulline in target proteins in an irreversible manner. PAD-mediated deimination can cause structural and functional changes in target proteins, allowing for protein moonlighting in physiological and pathophysiological processes. PADs furthermore contribute to the release of extracellular vesicles (EVs), which play important roles in cellular communication. In the present study, post-translationally deiminated protein and EV profiles of plasma were assessed in eight seabird species from the Antarctic, representing two avian orders: Procellariiformes (albatrosses and petrels) and Charadriiformes (waders, auks, gulls and skuas). We report some differences between the species assessed, with the narrowest EV profiles of 50–200 nm in the northern giant petrel Macronectes halli, and the highest abundance of larger 250–500 nm EVs in the brown skua Stercorarius antarcticus. The seabird EVs were positive for phylogenetically conserved EV markers and showed characteristic EV morphology. Post-translational deimination was identified in a range of key plasma proteins critical for immune response and metabolic pathways in three of the bird species under study; the wandering albatross Diomedea exulans, south polar skua Stercorarius maccormicki and northern giant petrel. Some differences in Gene Ontology (GO) biological and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways for deiminated proteins were observed between these three species. This indicates that target proteins for deimination may differ, potentially contributing to a range of physiological functions relating to metabolism and immune response, as well as to key defence mechanisms. PAD protein homologues were identified in the seabird plasma by Western blotting via cross-reaction with human PAD antibodies, at an expected 75 kDa size. This is the first study to profile EVs and to identify deiminated proteins as putative novel plasma biomarkers in Antarctic seabirds. These biomarkers may be further refined to become useful indicators of physiological and immunological status in seabirds—many of which are globally threatened.

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Section: Discussionmentioning

confidence: 99%

Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds

Phillips

Kraev

Lange

2020

Biology

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“…The successful sequencing of the goose genome opened a prelude to more detailed study of geese21. To date, RNA-Seq has been applied in geese, mainly based on the ovarian2223, spleen24, exocrine25, hepatic26, follicles27, and mixed tissues2. Identified genes are responsible for reproductive biology, development and metabolism processes, and laying performance.…”

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confidence: 99%

Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose

Yu¹,

Lou²,

Zhao³

2016

Sci Rep

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Broodiness, a maternal behavior and instinct for natural breeding in poultry, inhibits egg production and affects the poultry industry. Phenotypic and physiological factors influencing broodiness in poultry have been extensively studied, but the molecular regulation mechanism of broodiness remains unclear. Effective research strategies focusing on broodiness are hindered by limited understanding of goose developmental biology. Here we established the transcriptomes of goose follicles at egg-laying and broody stages by Illumina HiSeq platform and compared the sequenced transcriptomes of three types of follicles (small white, large white and small yellow). It was found that there were 92 up-regulated and 84 down-regulated transcription factors and 101 up-regulated and 51 down-regulated hormone-related genes. Many of these genes code for proteins involved in hormone response, follicular development, autophagy, and oxidation. Moreover, the contents of progesterone and estradiol in follicles were altered, and the autophagy levels of follicles were enhanced during the broody stage. These results suggest that hormone- and autophagy-signaling pathways are critical for controlling broodiness in the goose. We demonstrated that transcriptome analysis of egg-laying and broody Zhedong white goose follicles provided novel insights into broodiness in birds.

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“…This study found that the relative expression levels of ENO1 in the ovaries of laying geese compared with those of pre-laying geese were increased by 2.34±0.67 fold. It has been found that the mRNA concentrations of ENO1 in the livers of laying geese were higher than pre-laying geese (Yen et al, 2009). We propose that the rapid growth and development of avian ovarian follicle also need consume glucose by the pathway of glycolytic as the dynamics of avian ovarian follicle development.…”

Section: Resultsmentioning

confidence: 95%

Expression of Eleven Egg Performance-associated Genes in the Ovary of Zi Geese <i>Anser anser domestica</i>

Wang

Guo

et al. 2013

J. Poult. Sci.

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a These authors contributed equallyThe relative expression of eleven egg performance-associated genes including the tau-crystallin/α enolase (ENO1), ribosomal protein S27a (RPS27A), calmodulin 1 (CALM1), solute carrier family 25 A6 (SLC25A6), arylsulfatase A (ARSA), syndecan 2 (SDC2), ribosomal protein L10a (RPL10A), ornithine decarboxylase antizyme 1 (OAZ1), cytochrome c somatic variant 2 (CYCS), adenosine triphosphate synthase, H + transporting, α subunit, isoform 1 (ATP5A1) and the ring finger protein 130 (RNF130) in the ovaries of pre-laying and laying Zi geese were analyzed using quantitative real-time PCR (qRT-PCR). Comparison between pre-laying and egg-laying stages, the relative expression of ENO1, RPS27A, SLC25A6, ARSA, SDC2, RPL10A, OAZ1, CYCS and RNF130 were significantly upregulated (P＜0.05), whereas the CALM1 and ATP5A1 were highly significantly up-regulated (P＜0.01). This study established the primary foundation to understand the possible roles of these genes in the ovaries.Key words: egg performance-associated genes, geese, ovaries, qRT-PCR J. Poult. Sci., 50: 91-98, 2013 Introduction Zi geese (Anser anser domestica) are excellent layers (around 100 per year) with a superior feed-to-egg conversion ratio (Kang et al., 2009). This species breeds in Northeast China (HeiLongjiang and JiLin provinces). In our previous study, in order to study the molecular genetic mechanisms of egg laying and improve laying performance, suppression subtractive hybridization (SSH) and reverse dot-blot were employed to identify egg laying performance-associated genes in the ovaries. We have discovered that the expression of 18 known and 8 unknown gene fragments were higher in the ovaries of egg-laying geese than pre-laying geese (Kang et al., 2009 identified to be differentially expressed in the ovaries by SSH. Their expression were confirmed by using quantitative real-time PCR (qRT-PCR) in both egg-laying and pre-laying stages in the ovaries of the Zi geese. This results provided the primary foundation to understand the possible roles of these genes in egg formation in geese. Materials and Methods Geese and Tissue CollectionTwenty female Zi geese were selected on a local farm and were raised according to the program used in farm. All the geese were fed according to conventional farming (Kang et al., 2009). Ten geese were killed by exsanguination at 5-month-old (average BW＝3.0±0.2 kg) and the ovaries were treated at pre-laying stage. Another 10 geese were killed at 8-month-old (average BW＝3.5±0.2 kg) and the ovaries were treated at egg-laying stage. Ovaries samples, which comprised the whole ovary including the small and large yellow follicles, were rapidly removed, wrapped in foil, frozen in liquid nitrogen, and stored at −70℃ until analysis. Extraction of Total RNA and Quality ControlTotal RNA was prepared by the Trizol reagent (Invitrogen Corporation, CA, U.S.A.) according to the instructions of the manufacturer. The total RNA samples from pre-laying and laying ovaries (n＝10, for each) were pooled separately. Genom...

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Abundantly expressed hepatic genes and their differential expression in liver of prelaying and laying geese

Cited by 11 publications

References 57 publications

Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds

Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds

Transcriptome analysis of follicles reveals the importance of autophagy and hormones in regulating broodiness of Zhedong white goose

Expression of Eleven Egg Performance-associated Genes in the Ovary of Zi Geese <i>Anser anser domestica</i>

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