Abundant Expression in Vascular Tissue of Plant TAF10, an Orthologous Gene for TATA Box-binding Protein-associated Factor 10, in Flaveria trinervia and Abnormal Morphology of Arabidopsis thaliana Transformants on its Overexpression

Abstract

TAF10 is one of the TATA box-binding protein-associated factors (TAFs), which constitute the TFIID complex. We isolated a plant TAF10 ortholog from a Flaveria trinervia cDNA library, and named it ftTAF10. The ftTAF10 polypeptide contains a histone-fold motif, which is highly conserved among the TAF10s of other organisms. A transiently expressed green fluorescent protein (GFP) fusion protein was translocated into the nuclei of onion epidermal cells, suggesting that the ftTAF10 functions in nuclei. The transcript level was higher in stems and roots than in leaves, and in situ hybridization of F. trinervia seedlings revealed that the ftTAF10 transcript is accumulated abundantly in vascular tissues of hypocotyls, in the central cylinder of roots, and slightly in bundle sheath cells of leaves. Overexpression of ftTAF10 in Arabidopsis under the cauliflower mosaic virus 35S promoter caused two kinds of abnormal morphology, limitation of the indeterminate inflorescence and production of deformed leaves. These results indicate the possibility that ftTAF10 is a plant 'selective TAF' involved in the expression of a subset of vascular abundant genes, and that its appropriate gene expression is necessary for normal development.

A plastidial sodium-dependent pyruvate transporter

“…Poly-A1 mRNA was isolated and used to prepare single-strand radiolabelled cDNA as described 22 . Each radiolabelled probe was hybridized to duplicate nylon membranes containing approximately 9,000 phage clones of a F. trinervia leaf cDNA library 23 . Except for the major C 4 marker genes encoding phosphoenolpyruvate carboxylase (PEPC), pyruvate, orthophosphate dikinase (PPDK) and NADP-ME, we isolated 56 clones as novel C 4 -abundant signals, which were classified into 19 genes by determining their cDNA sequences.…”

Yeast two-hybrid map of Arabidopsis TFIID

“…For example, only AtTAF6 is expressed at detectable levels in mature pollen and in tricell microgametes (https://www.genevestigator.ethz.ch, Zimmermann et al 2004). In studies where TAF10 from Flaveria trinervia was overexpressed in Arabidopsis, two distinct phenotypes were evident (Furumoto et al 2005). The first was a terminal flower-like phenotype displaying limitation of the indeterminate inflorescence, the presence of chimeric floral organs and dwarf curled leaves.…”

ArabidopsisTAF1 is anMRE11‐interacting protein required for resistance to genotoxic stress and viability of the male gametophyte

“…The TFIID component TAF10 has also been implicated in floral development. Overexpression of both Flaveria trinervia TAF10 or AtTAF10 in Arabidopsis results in floral abnormalities in addition to defects in leaf development (Furumoto et al, 2005;Tamada et al, 2007). Insertional mutants with reduced AtTAF10 levels displayed shorter roots and a high incidence of arrested vegetative meristems (Tamada et al, 2007).…”