Abundant bacteria in the proximal and distal intestine of healthy Siberian sturgeons (Acipenser baerii)

Abstract

The gut microbiota plays a key role in animal health, including immune and metabolic homeostasis. Despite the economic relevance of the sturgeon, studies addressing the gut microbiome of this species are scarce and have focused only on the hindgut. The objective of this work was to use Illumina metabarcoding technology to compare the bacterial microbiomes of two different intestinal locations, the proximal intestine (small intestine) and the distal intestine (hindgut containing the spiral valve), of healthy Siberian sturgeons (Acipenser baerii) reared on two different farms in Spain. Although a high degree of interindividual variability was observed, certain differences between the anatomical parts and between the geographical locations were clear. The results show that the sequences corresponding to the most abundant taxa were the basis for clustering according to anatomical region (proximal and distal intestine), whereas the less abundant taxa were the basis for clustering according to the geographical location of the fish farms. Finally, the results also indicate the existence of certain bacteria that are present in the intestinal tracts of all the fish analyzed in this study; this information may be useful in future studies aiming to establish differences based upon fish health or disease.

Taxonomic and Functional Characteristics of the Gill and Gastrointestinal Microbiota and Its Correlation with Intestinal Metabolites in NEW GIFT Strain of Farmed Adult Nile Tilapia (Oreochromis niloticus)

“…Obvious differences exist in the microbial composition and structure of different tissues (skin, gill, and intestine) and digesta (feces) of fish [37]. In this study, the microbiota structures of four gastrointestinal sites were clearly separated, as shown in previous studies on wild tilapia [32] and other animals [33][34][35][36]. Such significant differences in microbial structure led to significant differences in microbial function, which could be attributed to the functional heterogeneity of the gastrointestinal tract, because the stomach is the main site of diet fermentation and the hindgut plays an important role in nutrient absorption [38].…”

“…To date, the distinct distribution of the microbial communities in different gastrointestinal segments has already been reported in various aquatic animals, including shrimp [ 33 ], Atlantic salmon [ 34 ], Siberian sturgeon [ 35 ], and finless porpoise [ 36 ]. Obvious differences exist in the microbial composition and structure of different tissues (skin, gill, and intestine) and digesta (feces) of fish [ 37 ].…”

Effects of dietary LNA/LA ratios on growth performance, tissue fatty acid composition and immune indices in Manchurian trout,Brachymystax lenok

“…Our results show that the activities of AKP, ACP and LZM in the intestine are affected by different dietary LNA/LA ratios. PI and DI possess different physiological and metabolic functions (Pérez et al, 2019).…”

“…Our results show that the activities of AKP, ACP and LZM in the intestine are affected by different dietary LNA/LA ratios. PI and DI possess different physiological and metabolic functions (Pérez et al., 2019). Though the PI and DI both are important for absorption, PI also plays an important role in immunity (Lin et al., 2019).…”

Nasal and Fecal Microbiota and Immunoprofiling of Infants With and Without RSV Bronchiolitis

“…Nasal samples (1 g) from each infant were used for DNA extraction, which was performed as described (Pérez et al, 2019). Parallel, DNA was extracted from fecal samples (1 g) as already described (Lackey et al, 2019).…”