Abundance and diversity of sulfate-reducing bacteria in the sediment of the Zhou Cun drinking water reservoir in Eastern China

Abstract

ABSTRACT. Sulfate-reducing bacteria (SRB) play an important role in the sediments of bay areas, estuaries, and lakes. However, information regarding the genetic diversity of SRB in the sediments of drinking water reservoirs is scarce. In this study, we collected sediment samples from different sites in the Zhou Cun drinking water reservoir between April and June 2012. To explore the genetic diversity of SRB, we used the most-probable-number (MPN) method, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), and a cloning approach. The average content of acid-volatile sulfide at the deepest sampling site was 205.87 μg/g sediment. This result is often associated with a large abundance of SRB in the associated sediment. The highest MPN estimate (1.15 x 10 5 cells/g sediment) was detected in May at the deepest sampling site. The PCR-DGGE fingerprints of SRB based on the dissimilatory sulfite reductase beta subunit (dsrB) gene varied according to the different sampling sites and dates. The highest abundance of SRB in the sediments was predominantly found at the deepest sampling sites, (2015) as expected from the acid-volatile sulfide content. The dominant species were Desulfobulbus sp, Desulfobacterium sp, and uncultured sulfatereducing bacteria. Redundancy analysis revealed that organic matter and the concentrations of nitrogen and phosphorus in the sediments were significantly correlated with the diversity of SRB communities present. The results of this study provide a better understanding of the sulfate-reducing microbial species in the sediments of the Zhou Cun drinking water reservoir.

The Variation Characteristic of Sulfides and VOSc in a Source Water Reservoir and Its Control Using a Water-Lifting Aerator

“…Sulfides pollution is mainly concentrated in the lower water. According to the research of Yang [ 37 ], the freshwater habitat of the Zhoucun Reservoir shows a relative abundance of SRB, which is similar to the observation in marine sediments. A SRB count of approximately 4.50 × 10 4 cells/g sediment was estimated from the samples taken in 2013, which is a magnitude greater than in typical reservoirs.…”

Microbial Diversity and Community Structure of Sulfate-Reducing and Sulfur-Oxidizing Bacteria in Sediment Cores from the East China Sea

“…The diversity and community structure of SRB and SOB have, to date, been studied using denaturing gradient gel electrophoresis (DGGE) ( Varon-Lopez et al, 2013 ; Yang et al, 2015 ), restriction fragment length polymorphism ( Luo et al, 2011 ; Reed and Martiny, 2013 ), and clone library approaches ( Yang et al, 2013 ; Purcell et al, 2014 ). However, obtaining comprehensive information about the highly diverse SRB and SOB communities, particularly SRB and SOB from the prime habitats of marine sediments, using these methods is challenging ( Aoki et al, 2015 ; Zhang et al, 2016 ).…”

Environmental and Microbial Influences on Corrosion of Selected Types of Petroleum Industry Steel

“…and Desulfobacterium sp. dominated in a study on a drinking water reservoir in Eastern China (Akpan and Iliyasu, 2015;Yang et al, 2015). Akin to the current study where abundance varied but diversity was relatively the same from one environment to the other, a study on four different environments in Mexico concluded that the distribution of SRB genera was relatively consistent across the different environment but the abundance differed.…”