Abundance and diversity of nitrogen-removing microorganisms in the UASB-anammox reactor

Abstract

Anaerobic ammonium oxidation is considered to be the most economical and low-energy biological nitrogen removal process. So far, anammox bacteria have not yet been purified from cultures. Some nitrogen-removing microorganisms cooperate to perform the anammox process. The objective of this research was to analyze the abundance and diversity of nitrogen-removing microorganisms in an anammox reactor started up with bulking sludge at room temperature. In this study, the ammonia-oxidizing archaea phylum Crenarchaeota was enriched from 9.2 to 53.0%. Nitrosomonas , Nitrosococcus , and Nitrosospira , which are ammonia-oxidizing bacteria, increased from 3.2, 1.7, and 0.1% to 12.8, 20.4, and 3.3%, respectively. Ca . Brocadia , Ca . Kuenenia , and Ca . Scalindua , which are anammox bacteria, were detected in the seeding sludge, accounting for 77.1, 11.5, and 10.6%. After cultivation, the dominant genus changed to Ca . Kuenenia , accounting for 82.0%. Nitrospirae , nitrite oxidation bacteria, decreased from 2.2 to 0.1%, while denitrifying genera decreased from 12.9 to 2.1%. The results of this study contribute to the understanding of nitrogen-removing microorganisms in an anammox reactor, thereby facilitating the improvement of such reactors. However, the physiological and metabolic functions of the ammonia-oxidizing archaea community in the anammox reactor need to be investigated in further studies.

Anaerobic treatment of propylene glycol-contaminated domestic wastewater and microbial community profile at threshold ratio

“…polychlorobiphenyl compounds) is very crucial. On the other hand, Synergistetes nearly doubled its relative abundance; however Proteobacteria -a bacterial phylum reported as denitrifying bacteria-were no longer detected in the biomass sample taken on 154-d of operation which might be due to possible inhibition depending on the concentration of glycol available in domestic wastewater ( Chen et al., 2019 ). Watari et al.…”

Microbial community structure and ecology in sediments of a pristine mangrove forest

“…The detection of Planctomycetaceae in the mangrove sediments indicate that they are the probable drivers for this part of the nitrogen cycle in this environment. The family Caldilineaceae is commonly found in studies concerning nitrogen removal in wastewater consortia and have also been correlated with phosphorus removal in wastewater treatment systems (Chen et al 2019;Zhang, Xu, and Zhu 2017;Drury, Rosi-Marshall, and Kelly 2013) indicating the possibility that the group is active in N and P cycles in these sediments. The other families that present the capacity for nitrogen fixing in the sediments are Flavobacteriaceae (Kämpfer et al 2015) , Pseudomonadaceae (Özen and Ussery 2012) and Spirochaetaceae (Lilburn et al 2001) .…”

Immobilization of microorganisms in activated zeolite beads and alkaline pretreated straws for ammonium-nitrogen removal from urban river water

“…The fraction (%) of Proteobacteria (Pseudoxanthomonas, Limnohabitans, Thermomonas, Pseudomonas, Hydrogenophaga, Kaistobacter, Ramlibacter and Steroidobacter) in the groups 2#, 5#, 6# and 10# was 34, 34, 54 and 28%, respectively. Proteobacteria are the most common prokaryotes in the water environment, and they include photosynthetic bacteria, chemoautotrophic, and chemoheterotrophic bacteria found in both aerobic and anaerobic environments (Chen et al 2019;Baskaran et al 2020). Pseudomonas belongs to the proteobacteria, which includes both aerobic and heterotrophic denitrifying bacteria.…”

“…Pseudomonas belongs to the proteobacteria, which includes both aerobic and heterotrophic denitrifying bacteria. Chen et al (2019) identified genes from Thermomonas, Pseudomonas, and Hydrogenophaga as belonging to denitrifying bacteria. According to Baskaran et al (2020), Pseudomonas, Bacillus, and Hydrogenophaga are aerobic denitrifying bacteria that can denitrify under aerobic conditions.…”