Abundance and characterization of simple-sequence repeats (SSRs) isolated from a size-fractionated genomic library of Brassica napus L. (rapeseed)

Kresovich, S.; Szewc-McFadden, Amy K.; Bliek, S. M.; McFerson, James R.

doi:10.1007/bf00220879

Cited by 149 publications

(75 citation statements)

References 20 publications

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“…In cucurbit crops, watermelon also showed a higher frequency of GA/CT than GT/CA repeats (Jarret et al 1997) and the results were consistent with the present findings. A higher frequency of GA/CT repeats was observed in the genomes of rice (Wu and Tanksley 1993), wheat (Roder et al 1995), Arabidopsis (Bell and Ecker 1994), Brassica rapa (Suwabe et al 2002) and Brassica napus (Kresovich et al 1995). In contrast, a higher frequency of GT/CA repeats was …”

Section: Development Of Microsatellite Markersmentioning

confidence: 99%

Development of Microsatellite Markers in Melon (Cucumis melo L.) and Their Application to Major Cucurbit Crops.

et al. 2003

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We developed 31 sets of melon (Cucumis melo L.) microsatellite markers in this study. Primers for these markers were designed from clones containing simple sequence repeats (SSRs) of (GA/CT)n or (GT/CA)n isolated from a melon genomic library. These microsatellite loci were examined for the detection of alleles among twelve lines and cultivars from six varieties of C. melo. A total of 28 marker loci showed polymorphism by 2.0 % agarose TBE gel separation. And these markers were also examined for cross-species amplification applicable to nine other species in Cucurbitaceae. Among them, 13 marker loci were commonly detected within the major cucurbit crops, cucumber, pumpkin and watermelon using the present primer sets. The species to which the melon microsatellite markers would be most applicable was Momordica charantia (24 markers), followed by Cucumis sativus (20 markers) and Cucurbita maxima (18 markers). These conserved microsatellite loci could be used as anchor markers in studies on synteny in Cucurbitaceae.

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Section: Development Of Microsatellite Markersmentioning

confidence: 99%

Development of Microsatellite Markers in Melon (Cucumis melo L.) and Their Application to Major Cucurbit Crops.

et al. 2003

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“…After sequencing, 173 (71.2%) clones possessed microsatellites, and the remaining 70 clones (28.8%) were 'lost' because of the absence of a microsatellite. In several previous studies, inevitably some clones were sequenced that did not contain a microsatellite; in those studies, the proportions of not containing a microsatellite were, for example, 42% in Anisantha sterilis (Green et al 2000), 33% in rapeseed (Kresovich et al 1995), and 23% in olive (Carriero et al 2002).…”

Section: Isolation Of Microsatellitesmentioning

confidence: 99%

Development of new microsatellite markers and their application in the analysis of genetic diversity in lentils

et al. 2009

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This paper reports the development of new microsatellite markers for lentil (Lens culinaris subsp. culinaris) and their use for genetic diversity analysis of a lentil core collection developed at ICARDA (Aleppo-Syria). Fourteen new markers were developed from microsatellite flanking sequences of a genomic library from a cultivated lentil accession ILL5588. The core collection used comprises 109 accessions from 15 countries representing 57 cultigens (including 18 breeding lines) from 8 countries and 52 wild types of germplasm (L. culinaris subsp. orientalis, L. culinaris subsp. tomentosus and L. culinaris subsp. odemensis) from 11 countries. Total number of alleles detected across all microsatellite loci was 182, with a mean of 13 alleles per locus. The wild accessions were rich in alleles (151 alleles) compared to cultigens (114 alleles). The genetic diversity index for the microsatellite loci in the wild accessions ranged from 0.16 (for locus SSR28 in L. culinaris subsp. odemensis) to 0.93 (for locus SSR66 in L. culinaris subsp. orientalis) with a mean of 0.66, while in the cultigens genetic diversity varied between 0.03 (locus SSR28) and 0.87 (locus SSR207) with a mean of 0.65. The cluster analysis indicated two major clusters, mainly one with the cultigens and the other with the wild accessions.

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“…The development and application of technologies based on molecular markers provides powerful tools that are adequate enough to reveal polymorphisms at the DNA sequence level and are often robust to detect genetic variability within populations and between individuals (Kresovich et al, 1995;Reddy et al, 2002;Simmons et al, 2007). Many studies on genetic diversity of Musa have been conducted by applying different molecular markers including isozymes (Bhat et al, 1992), RFLP (Jarret et al, 1992), RAPD (Guimarães et al, 2009), AFLP (Wong et al, 2001) and SSR or VNTR (Kaemmer et al, 1997;Crouch et al, 1999;Ning et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Identification and Validation of Est-Derived Molecular Markers, Trap and Vntrs, for Banana Research

Garcia¹,

Talebi²,

Ferreira³

et al. 2011

Acta Hortic.

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The advent of high-throughput sequencing technology has generated abundant information on DNA sequences for the genomes of many plant species. Expressed Sequence Tags (ESTs), which are unique DNA sequences derived from a cDNA library and therefore representing genes transcribed in specific tissues or at some stage of development, are one type of DNA sequences highly available today for many important crop species. Molecular markers are used for bridging DNA sequence information with particular phenotypes and are useful tools for genotyping germplasm collections and also for tagging genes involved in desirable agronomic traits. In this sense, there is always a strong demand for suitable marker techniques to better utilise existing sequence information. A transcriptome database from banana (Musa spp.), DATAMusa, containing 42,724 ESTs from 11 different cDNA libraries and encompassing approximately 24 Mb of DNA sequence, was used in this study for the design of primers to PCR-amplify two types of EST-derived molecular markers, Variable Nucleotide Tandem Repeat (VNTR) and Target Region Amplification Polymorphism (TRAP). These primers were then validated against a panel of 14 diploid Musa genotypes and produced 32 (VNTR) and 119 (TRAP) alleles. Used separately or together, both types of markers were able to discriminate Musa genotypes from different genome background (A or B genomes). The TRAP alleles identified were derived from only one EST, while the VNTR alleles were derived from 12 unigenes. Based on the results of this study, EST-derived markers can be an important source of polymorphism to be used in genetic diversity and gene discovery studies in banana.

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Abundance and characterization of simple-sequence repeats (SSRs) isolated from a size-fractionated genomic library of Brassica napus L. (rapeseed)

Cited by 149 publications

References 20 publications

Development of Microsatellite Markers in Melon (Cucumis melo L.) and Their Application to Major Cucurbit Crops.

Development of Microsatellite Markers in Melon (Cucumis melo L.) and Their Application to Major Cucurbit Crops.

Development of new microsatellite markers and their application in the analysis of genetic diversity in lentils

Identification and Validation of Est-Derived Molecular Markers, Trap and Vntrs, for Banana Research

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