Abstracts of Presentations at the 2004 Fall Meeting of the Korean Society of Mycologyat Seoul Kyoyuk Munhwa Hoekwan, Seoul, Korea, October 21-22.

Lee, Yong-Bo; Na, Young-Hee; Baek, Suk-Bae; Lee, Jae Min; Choi, Kyung Dal; Shim, Jae Ouk; Basak, A. B.; Lee, Min Woong; Lee, Youn Su; Ryoo, Kyung Hwan; Lee, Hyang Burm; Kim, Kyung Mo; Kim, Chang‐Jin; Kwon, Ho Jeong; Bradner, Ron; Nevalainen, Helena; Lee, Jin Sung; Jung, Hack Sung; Lee, Hye Min; Ko, Mi Sun; Cho, Hye Sun; Park, Myung Soo; Yu, Seung Hun; Park, Hyuk-Gu; Ko, Han-Gyu; Park, Won-Mok; Hyun, Min-Woo; Choi, Ji-Seung; Jeon, Yong Jin; Chang, Kye-Seung; Kim, Jae‐Jin; Kim, Gyu-Hyeok; Sang, Mee Kyung; Kim, Jeong Gyu; Kim, Ki Deok; Kim, Su‐Young; Shrestha, Bhushan; Park, Youngjin; Jeong, Kwang-Ryel; Hong, In-Pyo; Nam, Sung‐Hee; Jung, I-Yeon; Sung, Gyoo-Byung; Nam, Hack-Woo; Kang, Seok-Woo; Hur, Hyeon; Lee, Min-Woong; Guo, Shun‐Xing; Lee, Kang-Hyo; Weon, Hang-Yeon; Seok, Soon‐Ja; Kwon, Soon‐Ik; Kim, Wan-Gyu; Kim, Yang-Sup; Jang, Chang Soon; Kim, Nam Kyu; Song, Jeong Young; Kim, Sun Ik; Yoo, Sang‐Jo; Kim, Hong Gi; Kim, Seong Hwan; Sung, Min-Sun; Yoon, Jihwan; Ko, Jung-Moon; Cha, Hyeon-Cheol; Zhang, Xuan Zhe; Kim, Byung Sup; Ryu, Ki‐Hyun; Cheon, Jung Uk; Han, Sang-Kuk; Park, Young-Jun; Jeong, Kwang-Ryul; Choi, Sung-Keun; Sung, Jae-Mo; Cheong, Jong-Chun; Jang, Kab-Yeul; Park, Jeong-Sik; Kong, Won-Sik; Yoo, Young-Bok; Kim, Kwang‐Ho; Wsten, Han A. B.; Lee, Yang-Soo; Heo, Byongsoo; Park, Seong-Cheol; Lee, Kang-Soo

doi:10.1080/12298093.2004.12015921

Cited by 9 publications

(10 citation statements)

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“…In contrast, ERK-1/2 appeared to be directly involved in the transcription process, because preincubation with U0126 strongly reduced CCL13 expression but had no effect on mRNA stability ( Figure 6, and results not shown). These findings are consistent with previous reports suggesting prominent roles for ERK-1/2 and p38 in chemokine expression and mRNA stabilization, respectively (40,42,43). Of note, although p38 activity is important for both constitutive and the OSM-induced CCL13 expression, ERK-1/2 activation entirely drives the constitutive production of CCL13 by RASFs but is only partially involved in the strongly increased transcription of CCL13 in normal SFs and RASFs in response to exogenous OSM (Figures 5A and C and Figure 6).…”

Section: Discussionsupporting

confidence: 93%

“…Previous studies suggested that the JAK/STAT pathway as well as the ERK and stress-activated MAPK p38 cascades are involved in the expression of many inflammatory chemokines (40,42,43). To clarify the molecular mechanism(s) underlying the specific increase in CCL13 mRNA expression through OSM stimulation in contrast to stimulation with IL-6, IL-1␤, or TNF␣, we compared the activation pattern of prominent signaling pathways in response to stimulation with the respective cytokines ( Figure 4A).…”

Section: Resultsmentioning

confidence: 99%

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Induction of CCL13 expression in synovial fibroblasts highlights a significant role of oncostatin M in rheumatoid arthritis

Hintzen¹,

Quaiser²,

Pap³

et al. 2009

Arthritis & Rheumatism

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Objective. To investigate the molecular mechanisms of CCL13/monocyte chemoattractant protein 4 (MCP-4) chemokine expression through proinflammatory cytokines in different primary human fibroblasts and the contribution of CCL13 to monocyte migration.Methods. Using RNase protection assays and enzyme-linked immunosorbent assays, we quantified the expression of CCL13 compared with that of CCL2/ MCP-1 in primary human fibroblasts. Boyden chamber assays were performed to determine the importance of CCL13 for migration of primary monocytes. Pharmacologic inhibitors as well as small interfering RNA knockdown approaches were used to investigate the signaling pathways regulating CCL13 expression.Results. The interleukin-6 (IL-6)-type cytokine oncostatin M (OSM) was a powerful inducer of CCL13 expression in primary synovial fibroblasts from patients with rheumatoid arthritis (RA) as well as those from healthy control subjects but not in other types of fibroblasts. Neither IL-6 nor tumor necrosis factor ␣ could stimulate the expression of CCL13 in synovial fibroblasts; IL-1␤ was a very weak inducer. Synovial fibroblasts from patients with RA constitutively produced low amounts of CCL13, which was partially dependent on constitutive production of OSM. By investigating the underlying molecular mechanism, we identified STAT-5, ERK-1/2, and p38 as critical factors involved in OSM-dependent transcription and messenger RNA stabilization of CCL13.Conclusion. In contrast to other prominent cytokines involved in the pathogenesis of RA, OSM can strongly up-regulate the expression of CCL13, a chemokine recently identified in the synovial fluid of patients with RA. Despite potent OSM-induced signal transduction in all types of fibroblasts analyzed, only synovial fibroblasts secreted CCL13, which might be indicative of tissue-specific imprinting of different fibroblasts during development.

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Section: Discussionsupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Induction of CCL13 expression in synovial fibroblasts highlights a significant role of oncostatin M in rheumatoid arthritis

Hintzen¹,

Quaiser²,

Pap³

et al. 2009

Arthritis & Rheumatism

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“…As nitrogen sources, Val, Leu, and Lys are known to affect the growth of microorganisms, as well as the production of -amylase. 19,20) Levels of Leu and Val were higher in the Cheonggukjang samples fermented with Bacillus licheniformis 58 and Bacillus l. 67 than in the sample fermented with Bacillus amyloliquefaciens CH86-1 at the final stage of fermentation. However, Lys slightly decreased from the beginning of fermentation.…”

Section: )mentioning

confidence: 95%

Metabolite Profiling of Cheonggukjang, a Fermented Soybean Paste, Inoculated with Various Bacillus Strains during Fermentation

Baek

Shim

Kwon

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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Metabolite profiling of Cheonggukjang inoculated with different Bacillus strains including Bacillus amyloliqueciens CH86-1, Bacillus licheniformis 58, and Bacillus licheniformis 67 during fermentation, was performed using gas chromatography-time of flight-mass spectrometry after derivatization, combined with multivariate statistical analysis. A total of 20 amino acids, 10 sugars, five sugar alcohols, and seven organic acids were identified in three Cheonggukjang samples. With fermentation time, most of the amino acids showed increasing amounts. On the other hand, most of the sugars including sucrose, fructose, and glucose decreasing patterns, and the amounts of organic acids varied. In order to observe differences in metabolites with fermentation time and inoculated Bacillus strains, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were carried out, respectively. On PCA plots, some sugars and organic acids including sucrose, fructose, glucose, mannose, succinic acid, and malonic acid, as well as most of the amino acids, contributed mainly to differentiation of the Cheonggukjang samples fermentation time. On the other hand, on PLS-DA, mannose, xylose, glutamic acid, and proline were mainly responsible for differentiating the Cheonggukjang among into various inoculated strains.

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“…Recombinant ade-180 noviruses expressing GFP and Cre recombinase were generated as [31]. These constructs were ligated into an E1 shuttle vector 182 and then linearized with PmeI.…”

mentioning

confidence: 99%

YAF2 promotes TP53-mediated genotoxic stress response via stabilization of PDCD5

Park

Choi

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Programmed cell death 5 (PDCD5) plays a crucial role in TP53-mediated apoptosis, but the regulatory mechanism of PDCD5 itself during apoptosis remains obscure. We identified YY1-associated factor 2 (YAF2) as a novel PDCD5-interacting protein in a yeast two-hybrid screen for PDCD5-interacting proteins. We found that YY1-associated factor 2 (YAF2) binds to and increases PDCD5 stability by inhibiting the ubiquitin-dependent proteosomal degradation pathway. However, knocking-down of YAF2 diminishes the levels of PDCD5 protein but not the levels of PDCD5 mRNA. Upon genotoxic stress response, YAF2 promotes TP53 activation via association with PDCD5. Strikingly, YAF2 failed to promote TP53 activation in the deletion of PDCD5, whereas restoration of wild-type PDCD5WT efficiently reversed the ineffectiveness of YAF2 on TP53 activation. Conversely, PDCD5 efficiently overcame the knockdown effect of YAF2 on ET-induced TP53 activation. Finally, impaired apoptosis upon PDCD5 ablation was substantially rescued by restoration of PDCD5WT but not YAF2-interacting defective PDCD5E4D nor TP53-interacting defective PDCD5E16D mutant. Our findings uncovered an apoptotic signaling cascade linking YAF2, PDCD5, and TP53 during genotoxic stress responses.

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Abstracts of Presentations at the 2004 Fall Meeting of the Korean Society of Mycologyat Seoul Kyoyuk Munhwa Hoekwan, Seoul, Korea, October 21-22.

Cited by 9 publications

References 0 publications

Induction of CCL13 expression in synovial fibroblasts highlights a significant role of oncostatin M in rheumatoid arthritis

Induction of CCL13 expression in synovial fibroblasts highlights a significant role of oncostatin M in rheumatoid arthritis

Metabolite Profiling of Cheonggukjang, a Fermented Soybean Paste, Inoculated with Various Bacillus Strains during Fermentation

YAF2 promotes TP53-mediated genotoxic stress response via stabilization of PDCD5

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