Abstracts of Papers Presented at the 32nd ORC A Congress

Etcheverry, S.B.; Narda, Griselda E.; Apella, M.C.; Baran, Ewelina; Möller, Helena; Schröder, Ulla; Driessens, F. C. M.; Theuns, H.M.; Borggreven, J.M.P.M.; Dijk, J.W.E. van; Weatherell, J.A.; Strong, Margaret K.; Robinson, Caitlin; Ralph, Jolyon; Harper, Dawn; Osborn, JE; Hefferren, J.J.; Clayton, Richard; Truin, G.J.; König, Klaus; Ruiken, H.M.H.M.; Vogels, A.L.M.; Elvers, J.W.H.; Johansson, Iréne; Ericson, Tobias

doi:10.1159/000260934

Cited by 2 publications

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“…[7] The children were brought in batches and clinically examined for dental caries using a good plain mouth mirror and a sharp explorer and were divided into two groups out of which 45 were without caries and 45 with decayed missing filled teeth (DMFT) 3-5. Dental caries was recorded as per criteria described by Klein.…”

Section: Methodsmentioning

confidence: 99%

Comparative evaluation of predictive value of three caries activity tests-snyder, lactobacillus count and cariostat in mixed dentition children with and without caries

et al. 2013

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Introduction:Dental caries is a disease with multifactorial etiology. Early detection and preventive measures are essential for caries control. Caries activity tests are one of the methods of early detection, but all these are based on microbiological study.Aims:Compare the sensitivity and specificity of Snyder test (ST), lactobacillus count (LBC) and Cariostat in mixed dentition children with and without caries.Materials and Methods:90 healthy mixed dentition children (79 boys and 11 girls) were selected and were divided into two groups, one with Decayed missing filled teeth (DMFT) 0 and another group with DMFT 3-5. The salivary samples were collected for ST and LBC and plaque samples were collected for Cariostat. Caries activity was assessed.Results:The data obtained were analyzed for sensitivity, specificity, positive predictive value, negative predictive value and over all accuracy.Conclusions:Our present study showed that the LBC was found to have a higher correlation in caries as the caries increased the count also increased with positive caries activity. A good correlation was found between Snyder and LBC for the caries activity 1, 2 and 3. But no correlation was seen for score 0. To conclude, a combination of all these tests was found to be good for assessing caries risk groups.

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Section: Methodsmentioning

confidence: 99%

Comparative evaluation of predictive value of three caries activity tests-snyder, lactobacillus count and cariostat in mixed dentition children with and without caries

et al. 2013

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Human Salivary Peroxidase and Bovine Lactoperoxidase are Cross-reactive

1990

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Peroxidases are abundant in nature, and the primary function of mammalian peroxidases is to catalyze the peroxidation of halides and pseudohalides. Previous studies have shown that antibodies raised against bovine lactoperoxidase moderately cross-react with human salivary peroxidase, a feature that has been used in the present study to examine epitopes common to the antigen and human salivary peroxidase. Polyclonal antibodies against a highly purified preparation of bovine lactoperoxidase were raised in rabbits, and their properties were examined. In double-immunodiffusion experiments, the two enzymes showed partial identity, and in competitive radioimmunoassay and enzyme-linked immunosorbent assay, lactoperoxidase replaced the labeled and coated antigen, while salivary peroxidase did not. However, salivary peroxidase from human and rat saliva samples and the purified enzyme in its non-reduced, reduced, and de-glycosylated forms were recognized by these antibodies, as analyzed by Western blot analysis and immunodetection. The major activity of these antibodies was directed against the protein core of the antigen. Immunodetection of the peptide fragments of bovine lactoperoxidase and human salivary peroxidase revealed structural differences in the two enzymes. These antibodies also precipitated an in vitro translation product from rat-parotid-gland cell lysate that, on SDS-PAGE, compared favorably with the expected molecular weight of a de-glycosylated peroxidase. The antibodies partly inhibited the enzyme activity of salivary peroxidase and the peroxidase in rat parotid gland lysate, but the enzyme activity of lactoperoxidase was not affected by addition of anti-lactoperoxidase IgG between 25 and 400 micrograms/mL. The enzyme activity remained unchanged in all samples when pre-immune IgG was used.

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Abstracts of Papers Presented at the 32nd ORC A Congress

Cited by 2 publications

References 0 publications

Comparative evaluation of predictive value of three caries activity tests-snyder, lactobacillus count and cariostat in mixed dentition children with and without caries

Comparative evaluation of predictive value of three caries activity tests-snyder, lactobacillus count and cariostat in mixed dentition children with and without caries

Human Salivary Peroxidase and Bovine Lactoperoxidase are Cross-reactive

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