Abstract:Laser confocal microscopy is a powerful tool for histochemical and cytochemical studies. It enables us to detect the fluorescencelabeled molecules in the specimens by optical. Simultaneous staining of nuclei with appropriate fluorescent dyes as counterstaining greatly facilitates the identification of the localization of the label. Recently, a variety of nucleic acid binding fluorescent dyes have been developed. We screened 20 nucleic acid-specific dyes for use in nuclear staining in laser confocal microscopy … Show more
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