Abstracts

Halmin, Märit; Chiesa, Flaminia; Vasan, Senthil K.; Wikman, A. W.; Norda, Rut; Rostaard, K. R.; Pedersen, O. P. Vesterager; Erikstrup, Christian; Nielsen, Kaspar René; Titlestad, Kjell-Einar; Ullum, Henrik; Hjalgrim, Henrik; Edgren, Gustaf

doi:10.1111/vox.12304

Cited by 2 publications

(3 citation statements)

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“…Anti-MUT (366T) bound strongly to 47 KHKRDTY 53 of GP(A-B-A): GP.Hut and GP(B-A-B): GP.Mur, GP.Hop, GP.Bun, GP.HF, and GP.Kip. The murine anti-MUT(CBC-412) published by Uchiwaka et al [27] bound to peptides ranging 44 TNDKHKRDTY 53 (personal communication). Donors can produce naturally occurring monospecific anti-Mur only [17].…”

Section: Discussionmentioning

confidence: 99%

“…Donors can produce naturally occurring monospecific anti-Mur only [17]. Human MoAb anti-Mur(HIRO-138) [26] and murine anti-Mur (CBC-431) [27] were published in 2000 and 2015 by Uchikawa et al, respectively. The anti-Mur (362T) we produced recognized 49 KRDTYPAHTA 58 , which contained a part of the region of 53 YPAHTANE 60 of GP(B-A-B): GP.Mur, GP.Hop, GP.Bun, and GP(A-B-A): GP.Dane [4,33].…”

Section: Discussionmentioning

confidence: 99%

“…Confirming glycophorin hybrids by genotyping every donor is impracticable. Several MoAbs have been published, such as anti-Mi a , anti-Mur, anti-Vw, and anti-MUT [25][26][27]. Recently, anti-Mi a (GAMA210) has been utilized in donor identifications [11,28].…”

Section: Introductionmentioning

confidence: 99%

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Universal Detection of Mia Antigen and Frequencies of Glycophorin Hybrids among Blood Donors in Taiwan by Human Monoclonal Antibodies against Mia (MNS7), Mur (MNS10), and MUT (MNS35) Antigens

et al. 2021

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Glycophorin hybrids such as GP.Mur are common in Southeast Asians. In Taiwan, clinically significant alloantibodies to the GP.Mur phenotype are the most important issue in blood banks. A large-scale screening of glycophorin hybrids in the Taiwanese population is urgently needed to ensure transfusion safety. Four clones of human hybridomas that secrete anti-Mia, anti-MUT, and anti-Mur were established by fusing human B-lymphocytes and myeloma cells (JMS-3). The specificity of each monoclonal antibody (MoAb) was characterized. Three MoAbs were applied on an Automated Pretransfusion Blood Testing Analyzer (PK7300/PK7400) for donor screening. Genotyping was performed to determine the detailed subgrouping of glycophorin hybrids. Four MoAbs are IgM antibodies. Anti-Mia (377T) binds to 46DXHKRDTYA54, 48HKRDTYAAHT57 peptides, and anti-Mia (367T) binds to 43QTNDXHKRD51 peptides (X indicates T, M, or K). Anti-Mur is reactive with 49KRDTYPAHTA58 peptides. Anti-MUT is reactive with 47KHKRDTYA54. A total of 78,327 donors were screened using three MoAbs, and 3690 (4.71%) were GP.Mur, 20 (0.025%) were GP.Hut, and 18 (0.022%) were GP.Vw. When the Mia antigen was introduced as routine screening, the frequency of Mi(a+) among blood donors in Taiwan was 4.66% (67,348/1,444,541). Mia antigen was implemented as a routine blood testing, and the results were labeled on all red blood cell (RBC) units.

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Section: Discussionmentioning

confidence: 99%