Abstract TP108: 2,2 Dipyridyl, An Iron Chelator, Does Not Reduce Intracerebral Iron Toxicity Or Improve Outcome After Intracerebral Hemorrhagic Stroke In Rats

Abstract

Background — After an intracerebral hemorrhage (ICH) iron is released from degrading erythrocytes over days, which causes secondary damage by increasing free radicals. Many studies show that iron chelators, such as deferoxamine, lessen injury, but not all studies support this. Hypothesis — The ferrous iron chelator, 2,2 Dipyridyl (DP), will decrease injury after ICH or intraparenchymal FeCl 2 infusion in rats. Experiment 1— Rats were given a collagenase-induced striatal ICH. In experiments 1 and 3, we tested whether behavior was improved (e.g., walking) from administering DP (25mg/kg/day, 12 hours after surgery for 3 days). They were euthanized after 7 days to determine non-heme iron levels in the brain. Experiment 2— Rats were injected with DP (20mg/kg) 6 hours after collagenase infusion and every 24 hours till euthanasia at 3 days for measuring edema. Experiment 3— After injecting FeCl 2 in the striatum rats were given DP (25mg/kg every 12 hr starting 2 hours prior to surgery for 3 days). The volume of tissue loss and Fluoro-jade staining (degenerating neurons) was measured. Results — DP did not improve behavioral or histological outcome or reduce edema in either the collagenase ICH or FeCl 2 model. DP also did not affect parenchymal non-heme iron level. Conclusion — Our data suggests that DP on its own is not an effective strategy for ICH.

GmYSL7 controls iron uptake, allocation, and cellular response of nodules in soybean

“…Next, we tested the response of soybean plants and nodulation to different Fe concentrations. To determine the appropriate concentrations of AA-DP (2,2'-bipyridine) to chelate free Fe 2+ (Caliaperumal et al, 2013), we treated soybean plants with different concentrations of AA-DP under low nitrogen and analyzed nodule number, shoot length, and primary root length at 28 DAI. The results showed that 200 and 500 μmol/L AA-DP treatments greatly reduced nodule number and slightly shortened shoot length; 200 μmol/L AA-DP treatment had no effect on primary root growth, while 500 μmol/L AA-DP inhibited root growth (Figure S1A-D).…”