Abstract P6-01-09: MiRNA-200b suppresses triple negative breast cancer metastasis by targeting ARHGAP18 and causing sustained Rho A activation

Wang, Z; Humphries, Brock A.; Li, Y; Yang, Chengfeng

doi:10.1158/1538-7445.sabcs16-p6-01-09

Cited by 2 publications

(1 citation statement)

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“…We found that miRNA-200b-3p was upregulated in the ovary in the three zebrafish comparisons. The role of this miRNA is not fully understood but it is known to be involved in many human cancers: kidney [81], prostate [82], and breast [83]. It is highly released in the serum of the anovulatory women diagnosed with polycystic ovary syndrome and suggested as a clinical marker [84].…”

Section: Discussionmentioning

confidence: 99%

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

Gelderen

Montfort

Álvarez‐Dios

et al. 2021

Preprint

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MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression in a wide variety of physiological processes, including those related to the reproductive system. Although in the last decade a plethora of miRNAs has been reported, the miRNA alterations occurred by environmental cues and their biological functions have not yet been elucidated. With the aim to identify epigenetic regulations mediated by miRNAs in the gonads in a climate change scenario, zebrafish (Danio rerio) were subjected to high temperatures during sex differentiation (18-32 days post fertilization, dpf), a treatment that results in male-skewed sex ratios. Once the fish reached adulthood (90 dpf), ovaries and testes were sequenced by high-throughput technologies. About 101 million high-quality reads were obtained from gonadal samples. Analyses of the expression levels of the miRNAs identified a total of 23 and 1 differentially expressed (DE) miRNAs in ovaries and testes, respectively, two months after the heat treatment. Most of the identified miRNAs were involved in human sex-related cancer. After retrieving 3’ UTR regions, ~400 predicted targets of the 24 DE miRNAs were obtained, some with reproduction-related functions. Their synteny in the zebrafish genome was, for more than half of them, in the chromosomes 7, 2, 4, 3 and 11 in the ovaries, chromosome 4 being the place where the predicted sex-associated-region (sar) is localized in wild zebrafish. Further, spatial localization in the gonads of two selected miRNAs (miR-122-5p and miR-146-5p) showed exclusive expression in the ovarian germ cells. The present study expands the catalog of sex-specific miRNAs and deciphers, for the first time, thermosensitive miRNAs in the zebrafish gonads that might be used as potential epimarkers to predict environmental past events.

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Section: Discussionmentioning

confidence: 99%

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

Gelderen

Montfort

Álvarez‐Dios

et al. 2021

Preprint

View full text Add to dashboard Cite

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Deciphering sex-specific miRNAs as heat-recorders in zebrafish

Gelderen

Montfort

Álvarez‐Dios

et al. 2022

Sci Rep

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In the last decade, a plethora of microRNAs (miRNAs) has been reported in a wide variety of physiological processes, including reproduction, in many aquatic organisms. However, miRNAome alterations occurred by environmental cues due to water temperature increment have not yet been elucidated. With the aim to identify epigenetic regulations mediated by miRNAs in the gonads in a climate change scenario, the animal model zebrafish (Danio rerio) were subjected to high temperatures during sex differentiation, a treatment that results in male-skewed sex ratios in the adulthood. Once the fish reached adulthood, gonads were sequenced by high-throughput technologies and a total of 23 and 1 differentially expressed miRNAs in ovaries and testes, respectively, were identified two months after the heat treatment. Most of these heat-recorder miRNAs were involved in human sex-related cancer and about 400 predicted-target genes were obtained, some with reproduction-related functions. Their synteny in the zebrafish genome was, for more than half of the predicted target genes, in the chromosomes 7, 2, 4, 3 and 11 in the ovaries, chromosome 4 being the place where the sex-associated-region (sar) is localized in wild zebrafish. Further, spatial localization in the gonads of two selected heat-recorder miRNAs (miR-122-5p and miR-146-5p) showed exclusive expression in the ovarian germ cells. The present study expands the catalog of sex-specific miRNAs and deciphers, for the first time, thermosensitive miRNAs in the zebrafish gonads that might be used as potential epimarkers to predict environmental past events.

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Abstract P6-01-09: MiRNA-200b suppresses triple negative breast cancer metastasis by targeting ARHGAP18 and causing sustained Rho A activation

Abstract: This abstract was not presented at the symposium.

Cited by 2 publications

References 0 publications

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

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