Abstract: P282 TARGETED DELIVERY OF STEM CELLS TO ATHEROSCLEROTIC ARTERIES USING IMMUNOLIPOSOMES

Geng, YJ; Klegerman, Melvin E.; Holland, Christie A.; McPherson, David D.

doi:10.1016/s1567-5688(09)70577-x

Cited by 1 publication

(1 citation statement)

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“…For those arteries that received ultrasound treatment, the transducer cable was connected to the amplifier when the Rh-ELIP bolus was visually confirmed to be exiting from the artery, and was left connected for 30 seconds [39], [40]. The artery was insonified with 1-MHz CW ultrasound at a peak-to-peak pressure of 0.49 MPa and the passive cavitation detector was used to verify the duration of the resulting stable cavitation.…”

Section: Methodsmentioning

confidence: 99%

Ultrasound-enhanced delivery of targeted echogenic liposomes in a novel ex vivo mouse aorta model

Hitchcock

Caudell

Sutton

et al. 2010

Journal of Controlled Release

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The goal of this study was to determine whether targeted, Rhodamine-labeled echogenic liposomes (Rh-ELIP) containing nanobubbles could be delivered to the arterial wall, and whether 1 MHz continuous wave ultrasound would enhance this delivery profile. Aortae excised from apolipoprotein-E-deficient (n = 8) and wild-type (n = 8) mice were mounted in a pulsatile flow system through which Rh-ELIP were delivered in a stream of bovine serum albumin. Half the aortae from each group were treated with 1-MHz continuous wave ultrasound at 0.49 MPa peak-to-peak pressure, and half underwent sham exposure. Ultrasound parameters were chosen to promote stable cavitation and avoid inertial cavitation. A broadband hydrophone was used to monitor cavitation activity. After treatment, aortic sections were prepared for histology and analyzed by an individual blinded to treatment conditions. Delivery of Rh-ELIP to the vascular endothelium was observed, and subendothelial penetration of Rh-ELIP was present in five of five ultrasound-treated aortae and was absent in those not exposed to ultrasound. However, the degree of penetration in the ultrasoundexposed aortae was variable. There was no evidence of ultrasound-mediated tissue damage in any specimen. Ultrasound-enhanced delivery within the arterial wall was demonstrated in this novel model, which allows quantitative evaluation of therapeutic delivery.

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Section: Methodsmentioning

confidence: 99%