Abstract P165: Urinary Amino Acid Profile And Cardiovascular Parameters In Overweight And Obese Brazilian Children

Abstract

Despite being thoroughly studied, there isn’t complete elucidation of mechanisms underlying obesity, and especially regarding overweight in children. Small molecule metabolites are promising candidates for understanding obesity′s phenotypes. Recent metabolic studies have advanced in discovery of biomarkers for better knowledge of mechanisms related to obesity and its comorbidities. In this cross-sectional study it was evaluated the profile of urinary amino acids of 110 children that were classified in into groups of nutritional status according to their BMI/A (Body Mass Index/age), as normal weight (NW) (n=45), overweight (OW) (n=21) and obese (OB) (n=44). The 24h urine samples were submitted to LC-MS/MS for evaluation of 47 amino acids using Amino Acids Analysis Kit (Zivak®, Turkey), corrected by creatinine concentration. Biochemical profile, cardiovascular parameters and anthropometric measurements were assessed following standard protocols. Girls showed a higher heart rate (G: 82bpm vs B:79bpm, p=0,01 ) than boys. Increased weight presented higher values of systolic blood pressure ( NW: 102.0, OW: 109.0, OB: 110.0; NW vs OW, p = 0.0001; NW vs OB, p = 0.0001 ) and diastolic blood pressure ( NW: 60.0, OW: 67.0, OB: 66.75, NW vs OW, p < 0.0001; NW vs OB, p < 0.0001 ). The following amino acids (expressed in nmol/mg of creatinine) were found in higher concentrations in children’s urine with overweight or obesity, when compared with NW group, glutamine ( NW: 1024.3 vs OW: 1309.2, p=0.05), tryptophan (NW: 85.65, OW:113.55, OB:109.36; NW vs OW p=0.01; OW vs OB p=0.004), phenylalanine (NW: 43.04, OW:49.66, OB:72.08; NW vs OB p=0.01; OW vs OB p=0.04), and Tyrosine (NW: 85.07, OW: 109.74, OB: 140.54; NW vs OB p<0.01; OW vs OB p=0.04). In contrast, sarcosine had reduced concentrations for groups with weight excess (OW: 78.7 vs OB: 62.3, p=0.04). Urine levels of glutamine and aromatic amino acids were higher in OW and OB groups. Tyrosine also showed a positive association with systolic blood pressure values. Thus, there was a positive correlation between levels of glutamine and aromatics amino acids and excess weight, suggesting that they may be candidates to biomarkers for obesity in children. Furthermore, obesity was directly related to increase in blood pressure values.