Abstract:Background: Now there are several approaches to isolate membrane proteins without detergents. Such approaches are promising as they guarantee the preservation of the lipid environment of the target proteins. One of such novel methods is based on the usage of a copolymer of styrene and maleic acid (SMA). Particles with small membrane fragments containing transmembrane proteins bounded by a polymer are called nanodisks (SMALPs).Methods: We chose HEAG2 with affine 1d4 tag at the C-terminus as an object of interes… Show more
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