Abstract:Background: Cryo-electron tomography (cryo-ET) is currently the only technique that can be used for quaternary structure determination of polyribosomal complexes under near physiological conditions. Here we describe, in more detail, the cryo-ET pipeline from a sample preparation for data processing from the poster talk "Cryo-ET structural analysis of polyribosomes from HeLa cells" by Zhanna A. Afonina. Methods: The investigations were carried out in a Titan Krios 60-300 TEM/STEM (FEI, USA), equipped with Falco… Show more
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