Abstract 4608: Survivin downregulation by α-santalol is not mediated through PI3K-Akt pathway in human breast cancer cells

Abstract

α-Santalol, a terpenoid found in sandalwood oil, has been shown to inhibit cancer cell growth in vitro by inducing apoptosis. This study was performed to investigate the anticancer properties of α-santalol associated with the induction of apoptosis in cultured MCF-7 (estrogen receptor (ER) positive, and wild type p53) and MDA-MB-231 (ER-negative and mutant p53) breast cancer cells. Expression of major proteins examined in the study were determined using standard Western blot protocol and analyzed by LICOR-Odyssey infra-red scanner. Total protein levels of survivin were confirmed by survivin ELISA kit. Cell viability was assessed by trypan blue dye exclusion assay, and caspase-3 activity was determined by caspase-3 (active) ELISA kit. Treatment of breast cancer cells for 6 and 9 hour time intervals with α-santalol (20, 40 μM) resulted in statistically significant concentration-dependent downregulation of survivin. pAkt levels were found to be slightly upregulated despite the downregulation of survivin. Pharmacological inhibition of the PI3K-Akt pathway did not result in a synergistic/additive increase in cell death or caspase-3 activity caused by α-santalol. The study reveals that survivin downregulation by α-santalol in breast cancer cells is not mediated through the PI3K-Akt pathway. Citation Format: AJAY BOMMAREDDY, KARRYN CRISAMORE, SARAH FILLMAN, SARAH BROZENA, JAMES STEIGERWALT, TERRA LANDIS, ADAM L. VANWERT, CHANDRADHAR DWIVEDI. Survivin downregulation by α-santalol is not mediated through PI3K-Akt pathway in human breast cancer cells. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 4608.

Antifungal and Cytotoxic Activities of Sixty Commercially-Available Essential Oils

“…Santalum album essential oil had previously demonstrated in vitro cytotoxic activity on both MCF-7 and MDA-MB-231 cells [ 25 , 30 ], as well as several other tumor cell lines [ 31 ]. The antifungal and cytotoxic activities of sandalwood oils can be attributed to the high concentrations of α- and β-santalols [ 32 , 33 ].…”

Characterization of the Olfactory Receptor OR10H1 in Human Urinary Bladder Cancer

“…The natural occurring substances from sandalwood essential oils are used to mitigate irritable conditions of the bladder ( Ballabh et al, 2008 ) and have been studied as chemopreventive agents for decades ( Dwivedi and Abu-Ghazaleh, 1997 ). Santalols, the main constituents of sandalwood oil, inhibit and destabilize tubulin polymerization in oral cancer cells, prostate cells and breast cancer cells and cause apoptosis and cell cycle arrest in a concentration-dependent manner ( Saraswati et al, 2013 ; Bommareddy et al, 2015 ; Lee et al, 2015 ). Therefore, we analyzed the effect of Sandranol on the cell cycle and cell morphology of BFTC905 cells and showed a reduction in adherens junction formation, as indicated by T-Cadherin staining.…”

Effects of celastrol on enhancing apoptosis of ovarian cancer cells via the downregulation of microRNA-21 and the suppression of the PI3K/Akt-NF-κB signaling pathway in an in vitro model of ovarian carcinoma

“…PI3K is a member of the lipid kinase family and is involved in the regulation of cellular metabolism, survival and proliferation. Akt is an important protein kinase downstream of PI3K, and its continuous activation is closely associated with tumor development (24,25). In breast and ovarian cancer, as well as additional malignancies, the PI3K/Akt pathway has been observed to be resistant to the induction of apoptosis by chemotherapy and radiotherapy (26,27).…”