Abstract 4451: The effects of anthocyanins from the fruit of Vitis coignetiae Pulliat on NF-κB and NF-κB-regulated gene expressions in human lung cancer cells

Abstract

Vitis coignetiae Pulliat (Meoru in Korea) has been used as a remedy for inflammatory diseases and various cancers. We isolated anthocyanins from Meoru. We previously suggested that anthocyanins from fruits of Vitis coignetiae Pulliat (Meoru in Korea) should have anti-invasive effects through suppression of NF-κB activation. Here, we investigated their effects on NF-κB-regulated gene products and cellular responses in human lung cancer cells. The anthocyanins inhibited NF-κB activation in a dose-dependent manner. TNF-α augmented proliferation, migration and invasion of A549 cells. The anthocyanins inhibited the augmented proliferation, migration and invasion of the cancer cells by TNF-α. We also found that the anthocyanins suppressed NF-κB-regulated proteins involved in caner proliferation, metastasis and anti-apoptosis. The anthocyanins inhibit NF-κB activity by inhibiting IκBα phosphorylation. Taken together, this study suggested that the anthocyanins isolated from fruits of Vitis coignetiae Pulliat inhibit the NF-κB activation and downstream proteins, which may be a factor in their anticancer activities of fruit of Vitis coignetiae Pulliat. Keywords: anthocyanins, Vitis coignetiae Pulliat, NF-κB, cancer * This study was supported by a grant from the National R&D Program for Cancer Control, Ministry for Health, Welfare and Family affairs, Republic of Korea (0820050) Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4451.

Blueberry Malvidin-3-galactoside Suppresses Hepatocellular Carcinoma by Regulating Apoptosis, Proliferation, and Metastasis Pathways In Vivo and In Vitro

“…Masoudi and Movahedi reported that blueberry anthocyanins exhibited anticancer activity by suppressing cell proliferation and functioned as anti-invasive factors and chemo-inhibitors. Anthocyanins have also been reported to inhibit nuclear factor κB (NF-κB) activation and the expression of downstream proteins, which may be an important factor contributing to their antiproliferation properties in human lung cancer cells . Shin et al reported that Vitis coignetiae Pulliat anthocyanins induced the apoptosis of human colon cancer HCT-116 cells via activating the p38 mitogen-activated protein kinase (MAPK) pathway and suppressing the AKT pathway.…”

“…Anthocyanins have also been reported to inhibit nuclear factor κB (NF-κB) activation and the expression of downstream proteins, which may be an important factor contributing to their antiproliferation properties in human lung cancer cells. 15 Shin et al 13 reported that Vitis coignetiae Pulliat anthocyanins induced the apoptosis of human colon cancer HCT-116 cells via activating the p38 mitogen-activated protein kinase (MAPK) pathway and suppressing the AKT pathway. Similarly, mulberry anthocyanins were shown to activate PI3K/AKT pathways in HepG2 cells.…”