Abstract 3166: Rab11 regulates E-cadherin expression and induces cell transformation in colorectal carcinoma

Abstract

In the process of epithelial mesenchymal transition EMT, the disassembly of junctional adhesion complexes such as E-cadherin is a remarkable sign during changes in cell morphology and polarity. However, E-cadherin expression is dynamic, and is regulated by the cellular endocytic system; it is also involved in cell signaling mechanisms. In this study, we investigated the role of E-cadherin in colorectal tumors and the relationship with recycling endosome protein Rab11 in colon cell transformation. For tissue screening, the expressions of E-cadherin and Rab11 in colorectal tumors were identified by immunohistochemistry in 113 patients with colorectal carcinoma. For the in vitro cell experiment, GFP-tagged Rab11 plasmid was transfected into HT29 colon cells, E-cadherin expression and cell transformation were monitored by Western blot and confocal microscopy. In immunohistochemistry, the mean score of E-cadherin in tumor and normal tissues was 1.41 ± 0.06 and 1.08 ± 0.06 (p < 0.05). The mean score of Rab11 in tumor and normal tissues was 0.51 ± 0.05 and 0.18 ± 0.02 (p < 0.05). Synchronous overexpression of E-cadherin and Rab11 was noted in 74 patients (66.5%) with colorectal carcinoma. When GFP-tagged Rab11 plasmid was overexpressed in cultured colon cell line HT-29, the E-cadherin expression was up-regulated, and a fibroblast-like cell polarity was induced, which resulted in cell transformation. This study demonstrated the importance of the overexpression of Rab11 and E-cadherin in colorectal cancer. The results indicated that Rab11 together with E-cadherin might be potential markers for colorectal cancer progression and treatment. Citation Format: Yuan-Chiang Chung, Wan-Chen Wei, Shin-Han Huang, Chi-Min Shih, Chih-Ping Hsu, King-Jen Chang, Wei-Ting Chao. Rab11 regulates E-cadherin expression and induces cell transformation in colorectal carcinoma. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3166. doi:10.1158/1538-7445.AM2014-3166

The transcription factor Rreb1 regulates epithelial architecture, invasiveness, and vasculogenesis in early mouse embryos

“…The cytoskeleton mediates vesicular trafficking, which can also regulate E-CADHERIN localization (Aiello et al, 2018;X. Y. Chen et al, 2003;Chung et al, 2014;X. Liang et al, 2015;Mary et al, 2002;Pilot, Philippe, Lemmers, & Lecuit, 2006;Sako-Kubota et al, 2014;Stehbens et al, 2006;Teng et al, 2005;Vasileva & Citi, 2018), and a large number of trafficking genes were upregulated in Rreb1 -/embryos.…”

The transcription factor Rreb1 regulates epithelial architecture and invasiveness in gastrulating mouse embryos

“…The cytoskeleton mediates vesicular trafficking, which can also regulate E-CADHERIN localization (Aiello et al, 2018; X. Y. Chen et al, 2003; Chung et al, 2014; X. Liang et al, 2015; Mary et al, 2002; Pilot, Philippe, Lemmers, & Lecuit, 2006; Sako-Kubota et al, 2014; Stehbens et al, 2006; Teng et al, 2005; Vasileva & Citi, 2018), and a large number of trafficking genes were upregulated in Rreb1 −/− embryos.…”