Abstract 2369: Molecular biological characterization of newly established epithelial ovarian cancer (EOC) cell lines : The cyclin-dependent kinase 1 pathway

Abstract

Background Gene expression pattern analysis comparing normal cells with cancer cells are performed by many competitors in the worldwide lab. Cancer has many different species in itself due to its individual differences, variations and heterogeneity. Therefore, it is necessary to spend more time in gene expression pattern analysis using microarray or whole genome sequencing. The purpose of this study was to identify genes and proteins that are highly differentially expressed in newly established epithelial ovarian cancer (EOC) cell lines, and to use this knowledge for the development of novel diagnostic and prognostic markers for EOC. We further performed the validation studies of several candidate genes. Results Comparison of gene expression patterns using microarray analysis enabled us to identify 84 genes that were commonly up-regulated and 132 genes that were down-regulated in cancer cell lines (up; > 2-fold, down; < 3-fold, P < 0.05). In 2-DE and MALDI-TOF/PMF, 31 up-regulated spots that had at least two-fold differences between the 3 EOC cell lines and HOSE cells used as controls were observed. 15 up-regulated genes in cell cycle related genes and 9 down-regulated genes in apoptosis related confirmed gene expression using quantitative RT-PCR. Pathway analysis was validated in cell cycle related genes in six EOC cell lines. Conclusions The data shows the aberrant expression of several cell cycle related genes in EOC and that Cyclin-dependent kinase 1 (CDK1) pathway may play a significant role in ovarian carcinogenesis, implying that CDK1 pathway could be used as a novel therapeutic target for ovarian cancer treatment. Citation Format: wookyeom yang, hanbyoul Cho, hayeon Shin, eunju Lee, hyunja Kwon, sol Kim, jaehoon Kim. Molecular biological characterization of newly established epithelial ovarian cancer (EOC) cell lines : The cyclin-dependent kinase 1 pathway. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2369. doi:10.1158/1538-7445.AM2014-2369