Abstract 1401: SYK promotes tumor progression in ovarian cancer cell lines

Sultan, Aya; Wang, Cindy Y.; Durán, George E.; Francisco, E. Brian; Berek, Jonathan S.; Šikić, Branimir I.

doi:10.1158/1538-7445.am2011-1401

Cited by 2 publications

(2 citation statements)

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“…Although no significant survival association was observed in these three genes, their potential biological or clinical relevance were confirmed by the evidences excavated from related researches. Especially, cancer genes, TRAT1 and SYK, have been validated as being significantly associated with lethal ovarian cancer, driving malignant transformation of ovarian cancer 32 33 34 . Taken together, the network-based module analysis has the ability to provide a deeper understanding of the characteristics of biomarker beyond biomarker discovery.…”

Section: Resultsmentioning

confidence: 99%

Network-based survival-associated module biomarker and its crosstalk with cell death genes in ovarian cancer

Jin

Miao

et al. 2015

Sci Rep

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Ovarian cancer remains a dismal disease with diagnosing in the late, metastatic stages, therefore, there is a growing realization of the critical need to develop effective biomarkers for understanding underlying mechanisms. Although existing evidences demonstrate the important role of the single genetic abnormality in pathogenesis, the perturbations of interactors in the complex network are often ignored. Moreover, ovarian cancer diagnosis and treatment still exist a large gap that need to be bridged. In this work, we adopted a network-based survival-associated approach to capture a 12-gene network module based on differential co-expression PPI network in the advanced-stage, high-grade ovarian serous cystadenocarcinoma. Then, regulatory genes (protein-coding genes and non-coding genes) direct interacting with the module were found to be significantly overlapped with cell death genes. More importantly, these overlapping genes tightly clustered together pointing to the module, deciphering the crosstalk between network-based survival-associated module and cell death in ovarian cancer.

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Section: Resultsmentioning

confidence: 99%

Network-based survival-associated module biomarker and its crosstalk with cell death genes in ovarian cancer

Jin

Miao

et al. 2015

Sci Rep

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“…SYK is upregulated in human prostate cancer and is associated with malignant progression (Ghotra et al 2015 ). SYK methylation was also correlated with poor overall survival of colorectal cancer (Yang et al 2013 ) and SYK is also overexpressed in ovarian cancer (Sultan et al 2011 ). Further studies will be needed to shed light on the use of SYK for targeted cancer therapy.…”

Section: Discussionmentioning

confidence: 99%

SYK-targeted dendritic cell-mediated cytotoxic T lymphocytes enhance the effect of immunotherapy on retinoblastoma

Jin

Zhou

Huang

et al. 2018

J Cancer Res Clin Oncol

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PurposeRetinoblastoma (RB) is the most common primary intraocular tumor in children. Chemotherapy is currently the main method of RB treatment. Unfortunately, RB often becomes chemoresistant and turns lethal. Here, we used in vitro cell immunotherapy to explore whether adoptive immunotherapy could be used as a potential treatment for RB. We focused on spleen tyrosine kinase (SYK), which is significantly upregulated in RB cells and serves as a marker for RB cells.MethodsUsing lentiviruses, we genetically modified dendritic cells (DCs) to express and present the SYK peptide antigen to cytotoxic T lymphocytes (CTLs) in vitro. We used SYK-negative cell lines (MDA-MB-231, MCF-10A, and hTERT-RPE1) and SYK-positive cell lines (MCF-7 and RB-Y79) to evaluate the specificity and cytotoxicity of DC presented CTLs using FACS, live-cell imaging, and RNA interference.ResultsThe cytotoxicity of CTLs induced by SYK-overexpressing DCs (SYK-DC–CTLs) was enhanced more than three times in SYK-positive cell lines compared with SYK-negative cell lines. DCs primed with SYK could drive CTL cytotoxicity against SYK-positive cell lines but not against SYK-negative cell lines. Moreover, SYK-silenced RB-Y79 cells successfully evaded the cytotoxic attack from SYK-DC–CTLs. However, SYK-DC–CTLs could target SYK overexpressed hTERT-RPE1 cells, suggesting that SYK is a specific antigen for RB. Furthermore, SYK-DC–CTL exhibited specific cytotoxicity against carboplatin-resistant RB-Y79 cells in vitro.ConclusionsOur data showed that SYK could be a potential immunotherapy target mediated by DCs. We propose SYK as a candidate target for treatment of chemoresistant RB.Electronic supplementary materialThe online version of this article (10.1007/s00432-018-2584-x) contains supplementary material, which is available to authorized users.

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Abstract 1401: SYK promotes tumor progression in ovarian cancer cell lines

Cited by 2 publications

References 0 publications

Network-based survival-associated module biomarker and its crosstalk with cell death genes in ovarian cancer

Network-based survival-associated module biomarker and its crosstalk with cell death genes in ovarian cancer

SYK-targeted dendritic cell-mediated cytotoxic T lymphocytes enhance the effect of immunotherapy on retinoblastoma

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