Absorption, Uptake and Tissue Affinity of High-Molecular-Weight Hyaluronan after Oral Administration in Rats and Dogs

Abstract

The purpose of this study was to determine the absorption, distribution and excretion of 99m technetiumlabeled, high-molecular-weight hyaluronan (( 99m Tc-P. Urinary and fecal excretion after 99m Tc-HA ingestion by rats showed 86.7-95.6% of radioactivity was recovered, almost all in feces. All tissues examined showed incorporation of radioactivity from 99m Tc-HA starting at 15 min and persisting for 48 h, in a pattern significantly different from 99m Tc-P. Wholebody scintigraphs and close-ups of the ventral chest region showed nonalimentary radioactivity from 99m Tc-HA concentrated in joints, vertebrae and salivary glands four hours after administration. Autoradiography of skin, bone and joint tissue pieces after 24 h showed incorporation of radioactivity from 99m Tc-HA, but not from 99m Tc-P. Conversely, absorption, distribution and excretion of 99m Tc was completely different from 99m Tc-HA, showing an expected pattern of rapid absorption and excretion in urine, with accumulation in thyroid glands, stomach, kidney and bladder. This report presents the first evidence for uptake and distribution to connective tissues of orally administered, high-molecularweight HA.

Structural characterization, sulfation and antitumor activity of a polysaccharide fraction from Cyclina sinensis

“…It has been reported that the absorption efficiency of polysaccharides increased with the decrease of molecular weight (Balogh et al, 2008;Zeng, Qin, Wang, Chi, & Li, 2008). In the present study, the improvement of antiproliferative activity might be due to the decreased molecular weight of CSPS-1 after sulfation.…”

Nondestructive Techniques to Evaluate the Characteristics and Development of Engineered Cartilage

“…Because of transport issues with higher molecular weight hyaluronan, the lower molecular weight GS and CS are more effective tracers. 7 Chondrocytes use GS for CS synthesis. Non-labeled native GS has therapeutic applications in osteoarthritis (OA) and radiolabeled GS was initially developed for scintigraphic detection of OA.…”

Supplements und Injektionen bei Arthrose und Knorpelschäden

“…In der Synovialflüssigkeit bewirkt sie eine Reibungsverminderung auf den artikulierenden Knorpeloberflächen. Die grundsätzliche Frage, ob die orale Einnahme bei einem sehr hohen First-passEffekt überhaupt das Gelenk erreichen kann, wurde durch eine Tierstudie mit radioaktiv markierter Hyaluronsäure positiv beantwortet [5]. Für die klinische Anwendung sind nur wenige Studien mit einer geringen Evidenz publiziert.…”