Absorption of Zinc from Dietary Casein Phosphopeptide Complex with Zinc in Rats Given a Soybean Protein-Based Diet.

Abstract

SummaryThe absorption of zinc (Zn) in the form of casein phosphopeptide (CPP) com plex was compared with that of its chloride form in rats given a soybean protein-based diet. We prepared 67Zn complex with CPP (67Zn-CPP). Male rats were given a diet containing ZnCl2 in the preliminary period. After overnight food deprivation, they were fed a test meal labeled 67Zn-CPP or 67ZnCl2 (4g Zn-free diet +0.12mg 67Zn) with 0.5mg Dysprosium (Dy) as a fecal marker. Feces were collected for 5d and analyzed for 67Zn isotopic enrichment and Dy concentration with an inductively coupled plasma mass spectrometer. Fecal Dy excre tion closely paralleled that of 67Zn, and more than 89% of the administered Dy was recov ered in the feces collected for 5d. Moreover, the Zn source did not affect the excretion pat tern of 67Zn. Since Dy and 67Zn were almost excreted within 3d after the administration, the apparent absorption of 67Zn was calculated from the pooled data for 3d. The source of Zn did not affect the apparent absorption of 67Zn. These results suggested that dietary Zn in the form of CPP complex did not show higher absorbability in rats.

Determination of true absorption and fecal endogenous loss of zinc in goats

“…Then, the 67 Zn concentration decreased rapidly 42 h after the administration, and then decreased gradually (Fig. There have been many studies on Zn absorption using stable isotope of Zn with Dy as unabsorbed marker in humans (Sheng et al 2005) and rats (Matsui et al 2002;Takasugi et al 2007). Fecal Dy concentration reached a peak 36 or 42 h after its administration and decreased gradually (Fig.…”

Casein phosphopeptides released by simulated gastrointestinal digestion of infant formulas and their potential role in mineral binding

“…CPP influence on iron and zinc absorption have been poorly documented and mainly limited to the tryptic fragment b- CN(1-25)4P (Aı¨t-Oukhatar et al, 1997;Matsui, Okumura, & Yano, 2002;Pe´re`s et al, 1998).…”

Does the addition of caseinophosphopeptides or milk improve zinc in vitro bioavailability in fruit beverages?

“…In rat pups, a positive effect was shown for CPPs from aqueous solutions of phytate and phytate-rich infant cereal with added CPPs (Hansen et al, 1996). In the opposite sense, Matsui, Okumura, and Yano (2002) in rats did not observe an effect of CPPs upon Zn absorption when administering them with a soybean protein-based diet. Based on the perfused rat loop model, the absorption of Zn increased, and negative interaction with Fe was minimized, when Zn was ingested as a complex with b-CN(1-25) (Pèrés et al, 1998).…”

“…We did not observe an increase in Zn availability due to the presence of CPPs, though it must be kept in mind that in this study the rats were perfused with solutions containing the complex Zn-b-CN(1-25), and using a specific CPP, which could explain the differences found. On the other hand, Matsui et al (2002) reported no effect on Zn absorption when rats were fed a soybean protein-based diet (phytate content 3 g/kg with Zn: CPP ratio 3:1) containing Zn bound to CPPs (more than 90% as b-CN(1-28)) versus Zn chloride. These authors attributed their results to the fact that the rats were fed with a Zn-CPP complex, and that the molar ratio and amount of CPPs administered were small.…”