1970
DOI: 10.1126/science.167.3915.179
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Absorption of Proteins and Peptides in the Far Ultraviolet

Abstract: An absorption peak for the peptide bond at 187 nanometers has been confirmed; a protein assay at this wavelength allows quantitation of proteins in aqueous solution at concentrations between 0.1 and 25 micrograms per milliliter. Assays are conducted at 6 degrees C to take advantage of the reduction of end-absorption of water with temperature.

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Cited by 44 publications
(10 citation statements)
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“…As previously shown for growth zones of bones (Carlisle 1970, Landis et al 1986), the authors demonstrate that silicon, in the form of amorphous oligomerized silicic acid, is involved in the early steps of calcification at nucleation sites, serving as intermediary between polysaccharide-protein complexes and inorganic ions catalyzing the precipitation of a mineral phase (Matsko et al 2011). …”
Section: Molting Cycle Cuticle and Calcificationsupporting
confidence: 54%
“…As previously shown for growth zones of bones (Carlisle 1970, Landis et al 1986), the authors demonstrate that silicon, in the form of amorphous oligomerized silicic acid, is involved in the early steps of calcification at nucleation sites, serving as intermediary between polysaccharide-protein complexes and inorganic ions catalyzing the precipitation of a mineral phase (Matsko et al 2011). …”
Section: Molting Cycle Cuticle and Calcificationsupporting
confidence: 54%
“…The role of Si in bioactive processes involved in new bone tissue formation is well known [173]. Carlisle first reported the presence Si traces during bone mineralization at early stages of calcification, and this has proven the influence of an Si-deficient diet in diseases such as osteoporosis [174]. Therefore, Si-substituted HAp (Si-HA) was chosen as an appropriate candidate for the preparation of an improved CaP material and was subsequently produced by chemical synthesis [175,176].…”
Section: Composites Of Hap and Inorganic Nanomaterialsmentioning
confidence: 99%
“…Silicon is an essential nutrient and silicon deficiency is associated with poor skeletal development [37, 39]. Carlisle performed electron probe microanalyses of various regions of bone and determined that silicon is twenty-five times more concentrated in immature osteoid than in mature bone [40]. Carlisle concluded that silicon plays a role in the initiation of the mineralization process.…”
Section: Lesser Known Nutritional Needsmentioning
confidence: 99%