Absorption of Acylated Anthocyanins in Rats and Humans after Ingesting an Extract ofIpomoea batatasPurple Sweet Potato Tuber

Abstract

We evaluated the absorbability of anthocyanins in humans and rats administered with a beverage prepared from an extract of the tuber of purple sweet potato (Ipomoea batatas Cultivar Ayamurasaki), or with an anthocyanin concentrate. Two major anthocyanin components, cyanidin 3-O-(2-O-(6-O-(E)-caffeoyl-beta-D-glucopyranosyl)-beta-D-glucopyranoside)-5-O-beta-D-glucopyranoside) and peonidin 3-O-(2-O-(6-O-(E)-caffeoyl-beta-D-glucopyranosyl)-beta-D-glucopyranoside)-5-O-beta-D-glucopyranoside), were detected in the plasma and urine of both rats and humans by HPLC or liquid chromatography/mass spectrometry (LC/MS). The plasma concentration of anthocyanins in humans reached a maximum 90 minutes after ingestion, and the recovery of anthocyanins in the urine was estimated as 0.01-0.03%. These results indicate that acylated anthocyanins could be selectively absorbed after ingesting food.

Intake of purple sweet potato beverage affects on serum hepatic biomarker levels of healthy adult men with borderline hepatitis

“…, are selectively detected in the blood and rapidly excreted in the urine of rats and humans (Suda et al, 2002;Harada et al, 2004;Kano et al, 2005). The urinary recovery rate of PSP anthocyanins is very low (0.01% in humans and 0.11% in rats); however, the radical-scavenging activity in the urine is elevated after the ingestion of the PSP beverage (Kano et al, 2005).…”

“…Despite the complex chemical structure of PSP anthocyanins, they are rapidly absorbed into the body, detected in blood and rapidly excreted in the urine of rats and humans (Suda et al, 2002;Harada et al, 2004).…”

Determination of Acylated Anthocyanin in Human Urine after Ingesting a Purple-Fleshed Sweet Potato Beverage with Various Contents of Anthocyanin by LC-ESI-MS/MS

“…sop-5-glc content, 73 mg/125 ml). 7) In addition, there was no significant difference (p < 0:05) in the Pn 3-Caf . sop-5-glc concentration between men and women who ingested the same beverage (Student's t-test with logarithmic transformation of the data).…”

“…Determination of Acylated Anthocyanin in Human Urinestudy. 7) The concentration ranged from 3.4 to 56.3 mg/l of urine in volunteers who ingested beverage A, from 13.8 to 147.4 mg/l of urine in those who ingested beverage B, and from 12.6 to 187.5 mg/l of urine in those who ingested beverage C. It was found that Pn 3-Caf . sop-5-glc was absorbed into the blood stream even though the anthocyanin content in the beverage was lower than that reported in a previous paper (Pn 3-Caf .…”

“…sop-5-glc), was directly absorbed into the blood stream of rats and was present as an intact acylated form in the plasma. 6) Other researchers have reported that acylated anthocyanins of purple-fleshed sweet potato were absorbed by rats and humans, 3,7) although the number of subjects was small and dosedependence was not investigated. Furthermore, they have reported that only two anthocyanins, cyanidin 3-caffeoylsophoroside-5-glucoside (Cy 3-Caf .…”