Absorption, fluorescence, and quantum yield for growth in nitrogen‐limited Dunaliella tertiolecta

Sosik, Heidi Al; Mitchell, B. Greg

doi:10.4319/lo.1991.36.5.0910

Cited by 95 publications

(84 citation statements)

References 27 publications

(27 reference statements)

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“…Falkowski (1992) suggested that φ m may also be correlated with nutrient availability. Several laboratory studies have shown that nutrient limitation tends to lower quantum yield (Cleveland & Perry 1987, Sosik & Mitchell 1991, and field studies have demonstrated a negative correlation between φ m and distance from the nitracline (Cleveland et al 1989, Lizotte & Priscu 1994. In the present study, there was no distinct nitracline for the range of depths sampled, and φ m did not show any relationship with nitrate concentration (Fig.…”

Section: Quantum Yieldmentioning

confidence: 38%

Bio-optical characteristics of diatom and prymnesiophyte populations in the Labrador Sea

Stuart¹,

Sathyendranath²,

Head³

et al. 2000

Mar. Ecol. Prog. Ser.

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During the spring of 1996, phytoplankton samples were collected along a transect from South Wolf Island (Labrador) to Cape Desolation (Greenland). Dense blooms of diatoms were found over the shelf near the coast of Labrador, whereas high concentrations of the colony-forming prymnesiophyte Phaeocystis pouchetii were found close to Greenland. Phytoplankton samples were separated into 2 major groups (diatoms or prymnesiophytes) on the basis of chlorophyll (chl) chl c 3 /chl a ratios (determined by HPLC analysis), and the effects of species composition on the absorption and photosynthetic characteristics of these 2 high-latitude phytoplankton populations were studied. At all pigment concentrations and all wavelengths examined (apart from 623 nm), the diatom population had a much lower absorption coefficient than the prymnesiophyte population; this was attributed to an increased pigment-packaging effect in the larger diatom cells. Varying proportions of photoprotective pigments also influenced the absorption characteristics of these populations. The low specific-absorption coefficient of the diatom population resulted in a higher maximum photosynthetic quantum yield relative to that of the prymnesiophyte population. The initial slope of the photosynthesis-irradiance (P-E) curve (α B ) also appeared to be taxon-specific, with higher α B values being recorded for the smaller prymnesiophytes than for the larger diatom cells. The implications of speciesdependent variations in phytoplankton absorption coefficients for the retrieval of remotely-sensed chl a are discussed.

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Section: Quantum Yieldmentioning

confidence: 38%

Bio-optical characteristics of diatom and prymnesiophyte populations in the Labrador Sea

Stuart¹,

Sathyendranath²,

Head³

et al. 2000

Mar. Ecol. Prog. Ser.

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“…Therefore, we assume that, in laboratory cultures maintained at high concentrations, the background contributions to the absorption at 676 nm from substances other than chlorophyll a may be negligible, such that a* cm ¼ a* ci . In laboratory cultures, the magnitude of a* cm at the red peak near l 676 nm has been measured in the range [0.025,0.028] m 2 (mg Chl-a) 21 [13,21,[23][24][25][26]. We note that, those reports have been based on laboratory cultures of both large (e.g.…”

Section: Choice Of A* CImentioning

confidence: 95%

Retrieval of phytoplankton size from bio-optical measurements: theory and applications

Roy

Sathyendranath

Platt

2010

J. R. Soc. Interface.

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The absorption coefficient of a substance distributed as discrete particles in suspension is less than that of the same material dissolved uniformly in a medium-a phenomenon commonly referred to as the flattening effect. The decrease in the absorption coefficient owing to flattening effect depends on the concentration of the absorbing pigment inside the particle, the specific absorption coefficient of the pigment within the particle, and on the diameter of the particle, if the particles are assumed to be spherical. For phytoplankton cells in the ocean, with diameters ranging from less than 1 mm to more than 100 mm, the flattening effect is variable, and sometimes pronounced, as has been well documented in the literature. Here, we demonstrate how the in vivo absorption coefficient of phytoplankton cells per unit concentration of its major pigment, chlorophyll a, can be used to determine the average cell size of the phytoplankton population. Sensitivity analyses are carried out to evaluate the errors in the estimated diameter owing to potential errors in the model assumptions. Cell sizes computed for field samples using the model are compared qualitatively with indirect estimates of size classes derived from high performance liquid chromatography data. Also, the results are compared quantitatively against measurements of cell size in laboratory cultures. The method developed is easy-to-apply as an operational tool for in situ observations, and has the potential for application to remote sensing of ocean colour data.

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“…data), and most Synechococcus strains are capable of using urea (Waterbury et al 1986;Collier et al 1999). Increases in growth rate and cellular chlorophyll concentration are both well-established responses to a reduction in nutrient stress (e.g., Caperon and Meyer 1972;Sosik and Mitchell 1991), suggesting that the positive relationship between these two picoplankton groups might be founded in similar response to relief from nutrient stress. Clearly, simply considering nitrogen uptake differences is an oversimplification of the complexity of factors affecting these populations; however, it is a step in exploring the underlying reasons for the observed dynamics.…”

Section: Defining the Picoplankton Community And Interactions-mentioning

confidence: 99%

Assessing the dynamics and ecology of marine picophytoplankton: The importance of the eukaryotic component

Worden

Nolan

Palenik

2004

Limnology & Oceanography

489

420

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We assess population dynamics of picophytoplankton groups (Յ2 m diameter; Prochlorococcus, Synechococcus, and picoeukaryote) at a Pacific Ocean coastal site in the Southern California Bight. Weekly sampling (August 2000 to January 2002), dilution experiments, and flow cytometric analysis were combined with an instrument-specific calibration for cell size determination, allowing biovolume and carbon biomass estimation. Synechococcus was almost always numerically dominant, accounting for 60 Ϯ 12% of the total picoplankton cells over time. It had moderately high growth rates (0.52-0.86 d Ϫ1 ) and was subject to low grazing mortality (Ϫ0.14 to Ϫ0.39 d Ϫ1 ). Prochlorococcus growth and mortality rates were roughly balanced (0.33 Ϯ 0.14 d Ϫ1 and Ϫ0.36 Ϯ 0.06 d Ϫ1 , respectively). Picoeukaryotes had the highest growth rates (0.71-1.29 d Ϫ1 ) and were responsible for, on average, 76% of net carbon production (NCP), amounting in up to 32.05In order to better define the eukaryotic component of these populations, an isolate was characterized via small subunit rRNA gene sequencing, transmission electron microscopy, and growth experiments and was identified as the prasinophyte Ostreococcus, not previously known to the Pacific Ocean. Our results show that although picoeukaryotes do not stand out as particularly important players in this system on the basis of cell abundance, they dominate in terms of picophytoplankton biomass and trophic transfer potential of carbon in this size class.

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Absorption, fluorescence, and quantum yield for growth in nitrogen‐limited Dunaliella tertiolecta

Cited by 95 publications

References 27 publications

Bio-optical characteristics of diatom and prymnesiophyte populations in the Labrador Sea

Bio-optical characteristics of diatom and prymnesiophyte populations in the Labrador Sea

Retrieval of phytoplankton size from bio-optical measurements: theory and applications

Assessing the dynamics and ecology of marine picophytoplankton: The importance of the eukaryotic component

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