Absorption, distribution, and excretion of [¹⁴C] patulin by rats

Abstract

Adult rats of both sexes were given a single oral dose of [14C] patulin and were sacrificed at various time intervals from 4 hr to 7 days following administration of the mycotoxin. Two groups of rats were employed; the treated group had been exposed to daily oral doses of unlabeled patulin (dissolved in pH 5.0 citrate buffer) in utero and for 41-66 wk after weaning, while the controls were given the buffer only throughout gestation and for 38-81 wk after weaning. Approximately 49% of the administered 14C radioactivity was recovered from feces and 36% from urine within 7 days after dosing. Most of the excretion of labeled material occurred within the first 24 hr. All of the 14C activity detected in the urine samples was either metabolites and/or conjugates of the original [14C]patulin. About 1-2% of the total radioactivity was recovered as 14CO2 from expired air. Carbon-14 radioactivity in various tissues and organs was determined throughout the 7 day period; the most significant retention site was the red blood cells.

Patulin-Induced Suicidal Erythrocyte Death

“…The concentrations required (2.5-5 µM) were similar to those (2.5 - 10 µM) effective in tumor cells [4]. In several countries the tolerance level of patulin contamination amounts to 50 µg/Kg (320 µM) [51] Following oral ingestion of radiolabelled patulin, some 36% of the label appeared in urine pointing to intestinal absorption [52]. Peritoneal administration of 1-3.75 mg/kg has been reported [53].…”

“…Peritoneal administration of 1-3.75 mg/kg has been reported [53]. From all tissues, erythrocytes displayed the stongest retention of the label [52]. …”

Patulin suppresses α1-adrenergic receptor expression in HEK293 cells

“…A study by Dailey et al in both male and female rats showed 36% PAT was recovered in the urine 7 days post-administration 64 . This study found PAT accumulated in blood rich organs specifically the kidney, liver, erythrocytes and spleen.…”

Patulin - a contaminant of Food and Feed : A review

“…Results of metabolic studies of C-patulin show that it is excreted principally via the faeces and urine. The major retention and storage site is the erythrocyte (Dailey et al, 1977;McKinley and Carlton, 1991). Patulin has an inhibiting effect on several biochemical parameters such AT-Pase, alkaline phosphatase, aldolase and hexokinase activity.…”