Absorption and utilisation of epimedin C and icariin from Epimedii herba, and the regulatory mechanism via the BMP2/ Runx2 signalling pathway

Huang, Xiaowen; Wang, Xuping; Zhang, Yang; Shen, Lifeng; Wang, Nani; Xiong, Xuefeng; Zhang, Li; Cai, Xiao-bin; Shou, Dan

doi:10.1016/j.biopha.2019.109345

Cited by 15 publications

(9 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Icariin was reported to inhibit osteoclast differentiation by suppreessing the expression of RANKL, 33 but the RANKL‐positive osteocytes in the icariin group were comparative to the untreated group, indicating that icariin had no further inhibitory effect on RANKL expression when used subsequently to zoledronic. The osteogenic enhancement of icariin via upregulation of BMP‐2 has been proven in the recent studies, 34,35 which was favored by the results of the present study: the positive osteocytes in I group were more than that of the U group though not significant, which indicating icariin can counteract the inhibition of BMP‐2 from zoledronic to some degree. So icariin may exert its bone protective effect via promotion of BMP‐2 and better mucosal healing.…”

Section: Discussionsupporting

confidence: 56%

Management of medication‐related osteonecrosis of jaw: Comparison between icariin and teriparatide in a rat model

Liu

Mattheos

Deng

et al. 2020

Journal of Periodontology

View full text Add to dashboard Cite

Background The aim of this study is to compare the effects of icariin and teriparatide on the treatment of medication‐related osteonecrosis of the jaw (MRONJ) using a rat model. Methods Fifty rats undergoing ovariectomy were randomly assigned to control group (n = 10) and the MRONJ model group (n = 40). Zoledronic acid (0.2 mg/kg) and dexamethasone (2 mg/kg) were injected into rats in the model group for 8 weeks while saline was applied in control group, then all rats underwent tooth extraction and bone defect. Eight weeks later, rats diagnosed with MRONJ (n = 33) were randomly distributed to icariin (n = 11), teriparatide (n = 11), and the untreated (n = 11) group, and rats received daily 150 mg/kg icariin, 20 µg/kg teriparatide, and no intervention, respectively, for 8 weeks. Then, mandibulars were dissected for later examination. Results Rats diagnosed with MRONJ (33/40) demonstrated significantly larger area of soft tissue wound and necrotic bone with higher ratio of empty bone lacuna. Area of soft tissue wound and ratio of empty bone lacuna were significantly decreased in the icariin group compared with the untreated group, while teriparatide group revealed significantly higher ratio of receptor activator of NF‐kB ligand (RANKL)‐positive osteocytes, smaller area of necrotic bone and lower ratio of empty lacuna. The two agents were related to higher expression of BMP‐2 in osteocytes but were not statistically significant. Conclusions Icariin benefits MRONJ in terms of the area of soft tissue wound and ratio of empty lacuna. Teriparatide activates expression of RANKL and reduces the area of bone necrosis and ratio of empty lacuna in a MRONJ lesion. The data suggest possible healing improvement in patients with MRONJ and further studies to prove the efficacy of icariin.

show abstract

Section: Discussionsupporting

confidence: 56%

Management of medication‐related osteonecrosis of jaw: Comparison between icariin and teriparatide in a rat model

Liu

Mattheos

Deng

et al. 2020

Journal of Periodontology

View full text Add to dashboard Cite

show abstract

“…There is very limited literature available on the anabolic effect of Epi C (as herba Epimedii extract), which has been used as TCM for the treatment of osteoporosis for many years [102]. Epi C and icariin are the major flavonoid glycosides existing in herba Epimedii, and several studies revealed that the flavonoids of Epimedii could regulate signaling pathways associated with bone and cartilage repair [52,[103][104][105]. Icariin, as a well-studied small molecule with similar chemical structure as Epi C, showed great potential in cartilage repair in in vitro and in vivo studies [52,106].…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Chondroprotective Effects of Vanillic Acid and Epimedin C in Human Osteoarthritic Chondrocytes

et al. 2020

View full text Add to dashboard Cite

In osteoarthritis (OA), inhibition of excessively expressed pro-inflammatory cytokines in the OA joint and increasing the anabolism for cartilage regeneration are necessary. In this ex-vivo study, we used an inflammatory model of human OA chondrocytes microtissues, consisting of treatment with cytokines (interleukin 1β (IL-1β)/tumor necrosis factor α (TNF-α)) with or without supplementation of six herbal compounds with previously identified chondroprotective effect. The compounds were assessed for their capacity to modulate the key catabolic and anabolic factors using several molecular analyses. We selectively investigated the mechanism of action of the two most potent compounds Vanillic acid (VA) and Epimedin C (Epi C). After identification of the anti-inflammatory and anabolic properties of VA and Epi C, the Ingenuity Pathway Analysis showed that in both treatment groups, osteoarthritic signaling pathways were inhibited. In the treatment group with VA, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling was inhibited by attenuation of the nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (IκBα) phosphorylation. Epi C showed a significant anabolic effect by increasing the expression of collagenous and non-collagenous matrix proteins. In conclusion, VA, through inhibition of phosphorylation in NF-κB signaling pathway and Epi C, by increasing the expression of extracellular matrix components, showed significant anti-inflammatory and anabolic properties and might be potentially used in combination to treat or prevent joint OA.

show abstract

“…The main component of the KXC is TWHF, which derives from Tripterygium hypoglaucum [ 26 , 27 ]. Herba Epimedii was widely used in the clinic to enhance bone fracture healing [ 28 ]. Fructus Lycii had many pharmacological and biological activities, such as anti-inflammation, antioxidation, antiapoptosis, immune regulation [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

The Derivative of Tripterygium wilfordii Hook F—Kunxian Capsule, Attenuated Rheumatoid Arthritis: A Systematic Review and Meta‐Analysis

Liu

Zhang

Chen

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

The study aimed to explore the efficacy and safety of Kunxian Capsule (KXC) in the treatment of rheumatoid arthritis (RA). The randomized controlled trials (RCTs) comparing the effects of KXC in patients with RA were included in this study. Weighted mean differences (MDs) were calculated for net changes by employing Review Manager meta-analysis software. Nine RCTs were included in the systematic review with a total of 747 patients. The overall effects showed that KXC alone or combined with disease-modifying antirheumatic and drugs decreased tender joint counts (P=0.02, MD = −1.07, 95% CI: −1.95 to −0.18), shortened duration of morning stiffness (P<0.0001, MD = −9.01, 95% CI: −13.08 to −4.93), lowered erythrocyte sedimentation rate (P<0.00001, MD = −5.27, 95% CI: −6.78 to −3.77), and reduced C-reactive protein (P<0.0001, MD = −5.04, 95% CI: −7.28 to −2.80). The most common adverse events were gastrointestinal disturbances and abnormal liver function. These results suggest that KXC is likely to be a more effective and safe candidate for treating RA compared with conventional therapies.

show abstract

Absorption and utilisation of epimedin C and icariin from Epimedii herba, and the regulatory mechanism via the BMP2/ Runx2 signalling pathway

Cited by 15 publications

References 29 publications

Management of medication‐related osteonecrosis of jaw: Comparison between icariin and teriparatide in a rat model

Management of medication‐related osteonecrosis of jaw: Comparison between icariin and teriparatide in a rat model

Anti-Inflammatory and Chondroprotective Effects of Vanillic Acid and Epimedin C in Human Osteoarthritic Chondrocytes

The Derivative of Tripterygium wilfordii Hook F—Kunxian Capsule, Attenuated Rheumatoid Arthritis: A Systematic Review and Meta‐Analysis

Contact Info

Product

Resources

About