Absorption and transport of dietary lipid in Pieris brassicae

Turunen, S.

doi:10.1016/0022-1910(75)90217-6

Cited by 38 publications

(4 citation statements)

References 12 publications

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“…In the midgut of Locusta migratoria , the products of TG hydrolysis are rapidly incorporated into phospholipids and neutral lipids (NL) (Weintraub & Tietz 1973). In addition, the midguts of Pieris brassicae and Aeshna cyanea incorporate triolein and oleic acid from the diet and transfer them to the haemolymph, mainly in the form of diacylglycerol (DG) (Turunen 1975, Bauerfeind & Komnick 1992, Grillo et al 2007). Soon after the haematophagous bug Rhodnius prolixus has a blood meal, phospholipids and DGs are released from the midgut to lipophorin (Lp) particles.…”

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confidence: 99%

Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus

Bittencourt‐Cunha

Silva-Cardoso

Oliveira

et al. 2013

Mem. Inst. Oswaldo Cruz

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In this study, we describe the fate of fatty acids that are incorporated from the lumen by the posterior midgut epithelium of Rhodnius prolixus and the biosynthesis of lipids. We also demonstrate that neutral lipids (NL) are transferred to the haemolymphatic lipophorin (Lp) and that phospholipids remain in the tissue in which they are organised into perimicrovillar membranes (PMMs). 3H-palmitic acid added at the luminal side of isolated midguts of R. prolixus females was readily absorbed and was used to synthesise phospholipids (80%) and NL (20%). The highest incorporation of 3H-palmitic acid was on the first day after a blood meal. The amounts of diacylglycerol (DG) and triacylglycerol synthesised by the tissue decreased in the presence of Lp in the incubation medium. The metabolic fates of 3H-lipids synthesised by the posterior midgut were followed and it was observed that DG was the major lipid released to Lp particles. However, the majority of phospholipids were not transferred to Lp, but remained in the tissue. The phospholipids that were synthesised and accumulated in the posterior midgut were found to be associated with Rhodnius luminal contents as structural components of PMMs.

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Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus

Bittencourt‐Cunha

Silva-Cardoso

Oliveira

et al. 2013

Mem. Inst. Oswaldo Cruz

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“…release labelled glycerides into the incubation medium does not demonstrate absence of a glyceride-synthesising system in the intestinal epithelium. Indeed recent observations suggest a capacity for diglyceride biosynthesis in homogenates of crop and midgut of P. americana (Hoffman and Downer, unpublished observations), and a similar capacity has been suggested in the intestinal epithelium of Locusta migratoria (Weintraub and Tietz 1973) and Pieris brassicae (Turunen 1975). It is interesting to note the release of some labelled diglyceride from a [l-14C]palmitate load during the first 2 h of crop incubation.…”

Section: Discussionmentioning

confidence: 86%

The crop as an organ of glyceride absorption in the American cockroach, Periplaneta americana L.

Hoffman¹,

Downer²

1976

Can. J. Zool.

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1976. The crop as an organ of glyceride absorption in the American cockroach, Periplaneta americana L. Can. J . Zool. 54: 1165-1 171.The potential of the crop to absorb the products of triglyceride hydrolysis was investigated in the American cockroach, Periplaneta americana. Isolated sacs of crop and midgut tissue were filled with test loads containing [I-'4C]palmitic acid or glyceryl tri[l-14C]palmitic acid and the efflux of label monitored. Label from both preparations was recovered primarily from the free fatty acid fraction. Inclusion of midgut Irpa.se enhanced the rate of 14C emux from test loads conraining IabeIled triglycerides. Comparison of the relative rates of lbC efflux from crop and midgut gacs shows that the greatest efflux occur5 from midgut, although the crop possesses the potential to effect abwrption of significant amounts of hydriilysed triglyceride. The capacity of crop to absorb Fatty acids was confirmed by monitonnp the evolution of I4CO2 from insects in which the crop was ligated anterior to the proventriculus before they were fed a test meal conlaining [ l-14C]pf miric acid. Ligated insects evolved 41 -5% of the I4CO, produced by nonligated insects. HOFFMAN, A. G. D., et R. G. H. DOWNER. 1976. The crop as an organ of glyceride absorption in the American cockroach, Periplaneta americana L. Can. J . Zool. 54: 1165-1 171.On a itudik le potentiel d'absurption des produits de I'hydmIyse de triplyctrides dans le jabot de la blatte F~riplnnetn nmericanu. Des sacsfaits de tissus du jabot et de I'intestin moyen ont d d remplis de ponions contenant des doses connues d'acide (I-14C]paImitique ou de glyceryl tri-wide [E-14C]palmitique: on mesurait ensuite la quantiti de marqueur liberc, Le marqueur des deux preparatiuns se retrouve sunout dans la fraction d'acides gras libres. L'addition de lipase venant de I'intestin rnoyen augmente le rythme de liberation du I4C des portions contenant les triglycir~des marquis. En camparant 3es taux relatifs de liberation de 14C dans les sacs formes de tissus du jabot el dans ceux formis de tissus de I'intestin rnoyen, on constate que I'intestin mayen en IibPre une plus grande quanritk que le jab. hien que ce dernier posside un potentiel sufisant pour absorber des quantitts importantes de triglyckride hydrolyse. On a mesure dwlement le potentiel d'absorption des acides gras du jabor en suivant l'evolution de "C02 chez des insectes dont on a ligature le jabot au des~us du proventricule, avant de les nourrir d'une diete contenant de i'acide If-I4C]palmitique. Chez les insectes ayanl subi la ligature. la quantite de 14C02 libtrk reprksente 41 3 % de la quantitk trouvde chez les insectes-tkmoins.[Traduit par le journal]

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“…Since PL constitutes an essential component of biological membranes (Chapman, 1975;Nagl, 1980), its distribution is observed in all the tissues throughout larva-to-adult development. However, its concentration varies (Turunen, 1975) (cf. tables), and because of this, the proportion of PL out of total lipids is variable, as also reported by Fast (1964) and Lee et al (1975) for other insects.…”

Section: Discussionmentioning

confidence: 99%

“…The phases associated with progeny production, migration and host selection all require lipids (Fulton and Romney, 1940). During larval development, fatty acid composition may change substantially (Nakasone and Ito, 1967;Turunen, 1975). In view of the large scale restructuring of larval tissues that occurs during metamorphosis, substantial alteration in the lipid profile may be expected to occur.…”

Section: Introductionmentioning

confidence: 99%

Stage- and sex-dependent changes in the lipid profile of Dysdercus koenigii (Heteroptera: Pyrrhocoridae) during development

Mishra

Singh

Kumar

2004

JTI

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Lipids were extracted from the plasma and gonads of fourth and fifth instars and adults of Dysdercus koenigii (Heteroptera: Pyrrhocoridae). Thin layer chromatography of the extracts revealed fractions of free fatty acids (FFA), monoglycerides (MG), diglycerides (DG), triglycerides (TG), phospholipids (PL), free cholesterol (FC) and esterified cholesterol (EC). There were distinct differences in the lipid profiles within sexes for both tissues during development. Phospholipid from female plasma showed significant fluctuation during development, while that from males remained constant. In female gonads, PL levels increased significantly from the fouth instar to the fifth instar. Monoglycerides were detected only in adult male plasma and adult female gonads, at very low levels. Plasma DG in males was found to increase significantly during development. Maximum gonadal DG was recorded in male fourth instars and female fifth instars. Triglycerides in female plasma declined from the fourth instar to the adult stage. In contrast, TG in gonads increased significantly in the fifth instar of both sexes. Plasma FFA of both sexes fluctuated during development. Free cholesterol was detected only in adult male plasma. Plasma EC increased from the fourth instar to the adult stage in males, and it was highest in gonads of fifth instars of both sexes.adultes. Le taux de EC du plasma augmente entre le quatrième stade juvénile et les adultes chez les mâles et il est plus élevé dans les gonades du cinquième stade juvénile chez les deux sexes.Mots clés: Dysdercus koenigii, développement, profil lipidique, TLC Abstract.

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Absorption and transport of dietary lipid in Pieris brassicae

Cited by 38 publications

References 12 publications

Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus

Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus

The crop as an organ of glyceride absorption in the American cockroach, Periplaneta americana L.

Stage- and sex-dependent changes in the lipid profile of Dysdercus koenigii (Heteroptera: Pyrrhocoridae) during development

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