Absorption and fluorescence studies on biological systems: Nucleic acid‐dye complexes

Absorption and Transport of Fluorescent Brighteners by Microorganisms

“…Much work has been done in the field of direct vital staining with acridine orange. This compound, however, has been found to be loosely bound to cytoplasmic nucleic acid in vitally stained monkey-kidney cells (Mayor, 1961), to be somewhat toxic to fibroblast cells at levels sufficiently high for cellular fluorescence (Wolf and Aronson, 1961), to have a reversible absorption spectrum which became apparent as the dye was eluted from Ehrlich ascites tumor cells (Loeser, West, and Schoenberg, 1960), and to have a highly reversible degree of binding within fibroblast cells which was to a large extent determined by the concentration in the medium (Hill et al, 1960). These latter workers also noted that it was necessary to make observations and photographs immediately, since exposure to light caused rapid bleaching and loss of crisp clear outlines.…”

Selected Topics in Photochemistry of Nucleic Acids, Recent Results and Perspectives

“…acridine orange (Loeser et al, 1960;Boyle et al, 1962;Borisova and Tumerman, 1964;Weill and Calvin, 1965;Lober, 1965;Tomita, 1967a;1967b;Lober, 1968;Borisova and Minyat, 1969;Killander and Rigler, 1969;Lober and Achtert, 1969;Yamabe, 1969;Kubota, 1970a;1970b;Romanovskaja et al, 1972), the alkaloid berberine (Hahn and Krey. 1971) and the trypanocidal dye ethidium bromide (Le Pecq and Paoletti, 1967;Sela, 1969;Maelicke, 1970;Rigler, 1970).…”