Absorption and emission spectral shifts of rose bengal associated with DMPC liposomes

Spectroscopic characterization and aggregation of azine compounds in different media

“…Nevertheless, these observations contradict the predictions of some authors who argue that increasing the lipophilicity of compounds, causes a rise in their aggregation tendencies [12,41].…”

“…However, in DMF there was a contrast between the absorption spectra of the dyes, and it is clear that in this solvent and PEG 400 the monobrominated derivative was less aggregated, which is an important property of PDT [12,34,35].…”

“…It has been reported that the formation of aggregate in solution produces significant changes in the absorption and emission spectra [11], thus impairing the photochemical response [12] and reducing the lifetimes of the excited state, most probably as a result of enhanced radiationless excited state dissipation and lowering of the quantum yields of the excited states and of the 1 O 2 generation [13].…”

Evaluation of the Photostability and Photodynamic Efficacy of Rose Bengal Loaded in Multivesicular Liposomes

“…For MVL-RB dispersions, the absorption spectra of RB were recorded after extraction from irradiated MVL using 1 % Triton X-100 in PBS. Baseline correction was carried out using a placebo of empty MVL dispersion diluted suitably with Triton X-100 in PBS to nullify any possible absorption arising from the soluble lipid [5]. During the irradiation process, samples were placed in a temperature controller system (Photon Scientific, Egypt), adjusted to 25 ± 0.2 °C to eliminate any heat effect induced by light.…”

“…The low lipid solubility and anionic nature of RB limit its capacity to cross biological barriers such as cell membranes. Consequently, its clinical application remains limited, although acylation of RB [4] and use of delivery carriers such as liposomes [5] have been suggested as alternative ways for its delivery to cells.…”