Absorption and Effectiveness of Orally Administered Low Molecular Weight Collagen Hydrolysate in Rats

Abstract

Collagen, a major extracellular matrix macromolecule, is widely used for biomedical purposes. We investigated the absorption mechanism of low molecular weight collagen hydrolysate (LMW-CH) and its effects on osteoporosis in rats. When administered to Wistar rats with either [(14)C]proline (Pro group) or glycyl-[(14)C]prolyl-hydroxyproline (CTp group), LMW-CH rapidly increased plasma radioactivity. LMW-CH was absorbed into the blood of Wistar rats in the peptide form. Glycyl-prolyl-hydroxyproline tripeptide remained in the plasma and accumulated in the kidney. In both groups, radioactivity was retained at a high level in the skin until 14 days after administration. Additionally, the administration of LMW-CH to ovariectomized stroke-prone spontaneously hypertensive rats increased the organic substance content and decreased the water content of the left femur. Our findings show that LMW-CH exerts a beneficial effect on osteoporosis by increasing the organic substance content of bone.

Inhibitory effect of collagen-derived tripeptides on dipeptidylpeptidase-IV activity

“…We confirmed that Pro-Hyp was not inhibitory against DPP-IV (data not shown). On the other hand, in studies using radiolabeled Gly-[ 14 C]Pro-Hyp, Watanabe-Kamiyama et al 18 reported that GlyPro-Hyp remained intact in the plasma when administered to Wistar rats. The two studies reported conflicting results regarding the fate of Gly-Pro-Hyp after oral administration.…”

An Overview of the Beneficial Effects of Hydrolysed Collagen as a Nutraceutical on Skin Properties: Scientific Background and Clinical Studies

“…Through the network of blood vessels, these collagen peptides and free amino acids are then distributed in the human body, in particular to the dermis, where it has been proven they can remain up to 14 days [32]. In the dermis, hydrolysed collagen has a dual action mechanism: 1) free amino acids provide building blocks for the formation of collagen and elastin fibres; 2) collagen oligopetides act as ligands, binding to receptors present on the fibroblasts' membrane and stimulate the production of new collagen, elastin and hyaluronic acid.…”

“…(Graph taken from Ref. [32]). collagen derived peptides (proline-hydroxyproline and glycine-proline-hydroxiproline) are absorbed through PEPT1 transporter [43].…”

“…Watanabe-Kamiyama et al [32] studied the distribution of collagen peptides to the skin and other tissue by means of an in vivo experiment, where proline or collagen peptides 14 C-labeled were administered to Wistar rats. Radioactivity was measured in the different tissues after ingestion of the collagen peptides and/or proline during 0 to 6 hours and until 14 days (Fig.…”

Collagen peptide modified carboxymethyl cellulose as both antioxidant drug and carrier for drug delivery against retinal ischaemia/reperfusion injury

“…An improving approach was modifying the cellulose with a stronger antioxidative group. Collagen peptide was known for its abundant physiological functions, such as chemotaxis, platelet aggregation, suppressing osteoclast differentiation and, in particular, protecting against oxidative free radical 17, 18, 19. Thus, the functionalization of carboxymethyl cellulose with collagen was expected to effectively improve its antioxidative capacity.…”