Absorptiometric determination of hydrogen peroxide in submicrogram amounts with leuco crystal violet and peroxidase as catalyst

Mottola, Horacio A.; Simpson, Benny E.; Gorin, George

doi:10.1021/ac60285a017

Cited by 139 publications

(85 citation statements)

References 12 publications

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“…The experimental method selected in the current study has been described as an accurate and sensitive means to determine pulpal penetration of hydrogen peroxide [1,13]. This method is based on the oxidation reaction of leucocrystal violet buffer solution by hydrogen peroxide that is catalysed by horseradish peroxidise to produce a colour to demonstrate the presence of hydrogen peroxide [1].…”

Section: Discussionmentioning

confidence: 99%

“…The pulp chamber of each tooth was then rinsed twice with 100µl portion of distilled water, placed that in the same glass test tube and was diluted with 3ml of distilled water. 100µl of 0.5mg/ml leukocrystal violet (sigma chemicals co.) and 50µl of 1mg/ml enzyme horseradish peroxidise (sigma chemicals co.) was also added to each test tube according to the method described by Mottola et al, [13]. This procedure was then repeated for each tooth [2,4,5].…”

Section: Methodsmentioning

confidence: 99%

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An in Vitro Spectrophotometric Analysis of the Penetration of Bleaching Agent into the Pulp Chamber of Intact and Restored Teeth

Patri¹,

Agnihotri

Rao

et al. 2013

JCDR

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Aim: To investigate the pulp chamber penetration of bleaching agent in intact teeth and teeth following restorative procedure.Methodology: Sixty extracted human incisors were selected and divided into 6 Groups. Four Groups were restored with hybrid composite resin and resin modified glass ionomer cement respectively, while 2 Groups were left intact. The Groups were then immersed in the treatment agents for 60 mins at 37°C. The optical density of the resultant blue solution obtained by adding leucocrystal violet and horseradish peroxidise to the acetate buffer solution present in the pulp chamber was measured spectrophotometrically and the data thus obtained was statistically analysed using ANOVA and paired t-test. Results:The amount of pulpal peroxide penetration in restored teeth was significantly higher than intact teeth (p< 0.001). the control Group showed no peroxide penetration. Groups restored with resin modified glass ionomer cement showed higher pulpal peroxide levels than Groups restored with hybrid composites, though the difference was not statistically significant (p = 0.52). Conclusion:Peroxide readily penetrates into the pulp through intact and restored teeth, with restored teeth showing higher pulpal peroxide levels than intact teeth. Teeth restored with resin modified glass ionomer cement showed higher pulpal peroxide level than teeth restored with composite resins.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

An in Vitro Spectrophotometric Analysis of the Penetration of Bleaching Agent into the Pulp Chamber of Intact and Restored Teeth

Patri¹,

Agnihotri

Rao

et al. 2013

JCDR

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“…The production of H 2 O 2 by S. sanguinis in liquid culture was measured as described previously (27). To measure the effect of S. mutans on the H 2 O 2 production of S. sanguinis, an overnight culture of S. sanguinis was diluted to ϳ10 7 cells/ml (OD 600 , ϳ0.025) and incubated anaerobically at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Competition and Coexistence betweenStreptococcus mutansandStreptococcus sanguinisin the Dental Biofilm

et al. 2005

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The human mucosal surface is colonized by the indigenous microflora, which normally maintains an ecological balance among different species. Certain environmental or biological factors, however, may trigger disruption of this balance, leading to microbial diseases. In this study, we used two oral bacterial species, Streptococcus mutans and Streptococcus sanguinis (formerly S. sanguis), as a model to probe the possible mechanisms of competition/coexistence between different species which occupy the same ecological niche. We show that the two species engage in a multitude of antagonistic interactions temporally and spatially; occupation of a niche by one species precludes colonization by the other, while simultaneous colonization by both species results in coexistence. Environmental conditions, such as cell density, nutritional availability, and pH, play important roles in determining the outcome of these interactions. Genetic and biochemical analyses reveal that these interspecies interactions are possibly mediated through a well-regulated production of chemicals, such as bacteriocins (produced by S. mutans) and hydrogen peroxide (produced by S. sanguinis). Consistent with the phenotypic characteristics, production of bacteriocins and H 2 O 2 are regulated by environmental conditions, as well as by juxtaposition of the two species. These sophisticated interspecies interactions could play an essential part in balancing competition/coexistence within multispecies microbial communities.

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“…Hydrogen peroxide production was determined immediately after irradiation by using the leuco crystal violet HRP method as described in ref. 17.…”

Section: Methodsmentioning

confidence: 99%

Detoxification of superoxide without production of H ₂ O ₂ : Antioxidant activity of superoxide reductase complexed with ferrocyanide

Molina-Heredia

Houée-Levin⊥

Berthomieu

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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Absorptiometric determination of hydrogen peroxide in submicrogram amounts with leuco crystal violet and peroxidase as catalyst

Cited by 139 publications

References 12 publications

An in Vitro Spectrophotometric Analysis of the Penetration of Bleaching Agent into the Pulp Chamber of Intact and Restored Teeth

An in Vitro Spectrophotometric Analysis of the Penetration of Bleaching Agent into the Pulp Chamber of Intact and Restored Teeth

Competition and Coexistence betweenStreptococcus mutansandStreptococcus sanguinisin the Dental Biofilm

Detoxification of superoxide without production of H ₂ O ₂ : Antioxidant activity of superoxide reductase complexed with ferrocyanide

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Absorptiometric determination of hydrogen peroxide in submicrogram amounts with leuco crystal violet and peroxidase as catalyst

Cited by 139 publications

References 12 publications

An in Vitro Spectrophotometric Analysis of the Penetration of Bleaching Agent into the Pulp Chamber of Intact and Restored Teeth

An in Vitro Spectrophotometric Analysis of the Penetration of Bleaching Agent into the Pulp Chamber of Intact and Restored Teeth

Competition and Coexistence betweenStreptococcus mutansandStreptococcus sanguinisin the Dental Biofilm

Detoxification of superoxide without production of H 2 O 2 : Antioxidant activity of superoxide reductase complexed with ferrocyanide

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Detoxification of superoxide without production of H ₂ O ₂ : Antioxidant activity of superoxide reductase complexed with ferrocyanide