Absorbable bioprosthesis for the treatment of bile duct injury in an experimental model

Montalvo-Javé, Eduardo E.; Barrera, Germán Eduardo Mendoza; Treviño, Arturo Zárate; Barba, Maria Cristina; Montalvo-Arenas, César; Mendoza, Fernando Rojas; Mancilla, Benjamín León; Pineda, Manuel A. García; Limón, Álvaro Jaime; Saavedra, Jorge Albores; Tapia-Jurado, Jesús

doi:10.1016/j.ijsu.2015.06.074

Cited by 6 publications

(4 citation statements)

References 16 publications

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“…In our model, we found that it was possible to replace only a selected portion of the liver with the xenogenic CMS, reducing clinical and surgical complications, compared with the decellularization process. The animal model was successfully reproducible, confirming that the CMS can be tailored to the size and form required, as previously reported ( 13 ). Regarding biocompatibility and the regeneration process, we selected PH as it is the most common model for regeneration.…”

Section: Discussionsupporting

confidence: 85%

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Xenoimplant of Collagen Matrix Scaffold in Liver Tissue as a Niche for Liver Cells

et al. 2022

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Hepatitis C virus-induced liver damage, chronic liver damage due to alcohol, and non-alcoholic liver disease-induced cellular alterations promote fibrosis, cirrhosis, and/or hepatocellular carcinoma. The recommended therapeutic option for advanced liver damage is liver transplantation. Extracellular matrix scaffolds have been evaluated as an alternative for tissue restoration. Studies on the biocompatibility and rejection of synthetic and natural scaffolds as an alternative to organ transplantation have been evaluated. Our group has recently described the xenoimplant of collagen matrix scaffold (CMS) in a rat model. However, no complete macroscopic and histological description of the liver parenchyma at the initial (day 3), intermediate (day 14), and advanced (day 21) stages has been obtained. In this study, we described and compared liver tissue from the CMS zone (CZ, CMS, and liver parenchyma), liver tissue from the normal zone (liver parenchyma close to the CMS), and basal tissue (resected tissue from the CMS implantation site). Our data strongly suggest that the collagen matrix xenoimplant is a good niche for hepatocytes, with no rejection, and does not affect liver function tests. The liver can regenerate after damage, but this capacity is inhibited in a chronic injury. At present, the use of CMS after liver damage has not been reported. This biomaterial could be a novel alternative in the field of regenerative medicine for liver diseases.

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Section: Discussionsupporting

confidence: 85%

“…Bile duct injury was induced in a porcine model, followed by choledochectomy and CMS implantation. Biosorption of the material occurred 6 months after the surgical procedure, with no tissue alteration or evidence of stenosis ( 13 ). In addition, we recently reported the implantation of CMS in the livers of rats.…”

Section: Introductionmentioning

confidence: 99%

Xenoimplant of Collagen Matrix Scaffold in Liver Tissue as a Niche for Liver Cells

et al. 2022

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“…The development and use of tubular scaffolding whether biological or synthetic, represents an area of opportunity as a viable therapeutic alternative for this type of injury. (Pérez et al, 2013;Montalvo-Javé et al, 2015) Pérez et al (2013) built three-dimensional collagen tubes coated with 2% agarose hydrogel. This material was implanted in 40 experimental animals substituting the native bile duct.…”

Section: Biomaterials Implant Overviewmentioning

confidence: 99%

Biomaterials and scaffolds in the medical-surgical area

Valderrama-Treviño

2018

Int. j. adv. appl. sci.

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“…The use of decellularized matrices for bile duct repair has focused on employing tubular tissues distinct from bile duct, such as ureter [ 9 ]. Furthermore, the animal sources employed in decellularization vary greatly and lack human anatomy resemblance [ 9 , 10 , 11 , 12 ]. Therefore, using porcine decellularized extrahepatic bile duct scaffolds may tackle the disadvantages before-mentioned, due to the anatomical similarities between species.…”

Section: Introductionmentioning

confidence: 99%

Perfusion Decellularization of Extrahepatic Bile Duct Allows Tissue-Engineered Scaffold Generation by Preserving Matrix Architecture and Cytocompatibility

et al. 2021

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Reconstruction of bile ducts damaged remains a vexing medical problem. Surgeons have few options when it comes to a long segment reconstruction of the bile duct. Biological scaffolds of decellularized biliary origin may offer an approach to support the replace of bile ducts. Our objective was to obtain an extracellular matrix scaffold derived from porcine extrahepatic bile ducts (dECM-BD) and to analyze its biological and biochemical properties. The efficiency of the tailored perfusion decellularization process was assessed through histology stainings. Results from 4’-6-diamidino-2-phenylindole (DAPI), Hematoxylin and Eosin (H&E) stainings, and deoxyribonucleic acid (DNA) quantification showed proper extracellular matrix (ECM) decellularization with an effectiveness of 98%. Immunohistochemistry results indicate an effective decrease in immunogenic marker as human leukocyte antigens (HLA-A) and Cytokeratin 7 (CK7) proteins. The ECM of the bile duct was preserved according to Masson and Herovici stainings. Data derived from scanning electron microscopy (SEM) and thermogravimetric analysis (TGA) showed the preservation of the dECM-BD hierarchical structures. Cytotoxicity of dECM-BD was null, with cells able to infiltrate the scaffold. In this work, we standardized a decellularization method that allows one to obtain a natural bile duct scaffold with hierarchical ultrastructure preservation and adequate cytocompatibility.

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Absorbable bioprosthesis for the treatment of bile duct injury in an experimental model

Cited by 6 publications

References 16 publications

Xenoimplant of Collagen Matrix Scaffold in Liver Tissue as a Niche for Liver Cells

Xenoimplant of Collagen Matrix Scaffold in Liver Tissue as a Niche for Liver Cells

Biomaterials and scaffolds in the medical-surgical area

Perfusion Decellularization of Extrahepatic Bile Duct Allows Tissue-Engineered Scaffold Generation by Preserving Matrix Architecture and Cytocompatibility

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