An absolute alignment measurement of an underlayer and overlayer of overlay mark enables an innovative overlay control by which each layer’s grid errors can be independently corrected, versus of a conventional relative overlay measurement and control. We demonstrate an absolute alignment measurement of stacked overlay marks such as Diffraction-Based Overlay (DBO) by adopting a unique method incorporated in a standalone, image-based alignment metrology system. An alignment accuracy of each layer is evaluated using product wafers by comparing alignment measurement result to the reference data. In conclusion, we were able to achieve R2>0.97 coefficient.