Absolute and relative accuracy of rapid urine tests for urinary tract infection in children: a meta-analysis

Williams, Gabrielle; Macaskill, Petra; Chan, Siew Foong; Turner, Robin; Hodson, Elisabeth M; Craig, Jonathan C.

doi:10.1016/s1473-3099(10)70031-1

Cited by 157 publications

(153 citation statements)

References 78 publications

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“…7 On contrary to this, Huysal et al found only 22% sensitivity. 8 Rest of the studies observed sensitivity above 50% with nitrite test.…”

Section: Discussionmentioning

confidence: 90%

“…8,12 Specificity for LE was only 25%, which is relatively lower than the observations made by others. [7][8][9]12,13 Positive predictive value is 69%, which is more as compared to that of Daghistani et al (44%). 9 Negative predictive value is 19% which is much lower as compared to other studies (Table 6).…”

Section: Discussionmentioning

confidence: 98%

“…9,[11][12][13] In this study, the specificity is 31%, which is lowest as compared to other studies. [7][8][9]12,13 Huysal et al attained a specificity of 99.3%. 8 Positive predictive value for nitrite test is 66% in the present study, which is comparable to that of Daghistani et al and Wammanda et al 9,10 However, the negative predictive value is only 18.5% in the present study, unlike in Daghistani et al, Wammanda et al and Hida et al who showed a negative predictive value of 86.2%, 80.8% and 89.9% respectively.…”

Section: Discussionmentioning

confidence: 99%

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Urinary tract infection in children: clinical aspects and utility of urine dipstick test

Mod¹,

Jeeyani²,

Shah³

2017

Int J Contemp Pediatr

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Background: Urinary tract infection (UTI) is a common genitourinary disease in children and accounts for significant morbidity. To prevent the potentially dangerous sequel, early detection of UTI in childhood is essential. This depends entirely on strong clinical suspicion and urine culture report. Recently, advances in investigations are taking place to diagnose UTI rapidly. Urine dipstick is one such bedside method to diagnose UTI. This study aims at studying the clinical features of UTI and evaluating sensitivity and specificity of urine dipstick in diagnosing UTI.Methods: This study was conducted at a tertiary care hospital in Vadodara from January 2013 to June 2014, on 60 children admitted with clinical suspicion of urinary tract infection. Along with study of clinical features, results of urine dipstick tests (Nitrite and Leukocyte esterase), urine microscopy and urine culture were compared. Accuracy of urine dipstick tests was assessed statistically by evaluating sensitivity, specificity, positive and negative predictive values considering urine culture as a gold standard.Results: Fever was found to be the most common presenting features (53%) in all the patients studied. The sensitivity of urine microscopy, Nitrite test and Leucocyte esterase test was 63.5%, 50% and 61% and specificity was 25%, 31% and 25% respectively. Combination of Nitrite and Leucocyte Esterase test had an improved sensitivity (68%).Conclusions: Urinary tract infection can present with variety of non-specific signs and symptoms especially in younger age. Dipstick test using Leucocyte Esterase and Nitrite together is better than urine microscopic examination alone. Urine culture as a confirmatory test still remains gold standard.

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“…7 On contrary to this, Huysal et al found only 22% sensitivity. 8 Rest of the studies observed sensitivity above 50% with nitrite test.…”

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Urinary tract infection in children: clinical aspects and utility of urine dipstick test

Mod¹,

Jeeyani²,

Shah³

2017

Int J Contemp Pediatr

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“…However, the majority of urine samples sent to the laboratory are negative. Screening methods (e.g., dip stick testing, automated urine flow cytometry, microscopy) for UTI are limited [15][16][17]. In addition, these tests are not being able to replace urine cultures because they neither identify the causal pathogen nor establish antibiotic susceptibility-guided antibiotic treatment.…”

Section: Discussionmentioning

confidence: 99%

Standardization of isothermal microcalorimetry in urinary tract infection detection by using artificial urine

et al. 2012

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“…14 Because a high frequency of bacterial contamination is associated with urine cultures, 15 the sterility of cultures was checked by performing PCR for bacterial 16S ribosomal RNA. In a preliminary set of experiments, Sanger sequencing of the PCR product and alignment against sequences present in 16S ribosomal RNA sequence National Center for Biotechnology Information database was performed in cultures.…”

Section: Set Up Of a Methods To Culture Rpcs From Urinementioning

confidence: 99%

Human Urine-Derived Renal Progenitors for Personalized Modeling of Genetic Kidney Disorders

Lazzeri¹,

Ronconi

Angelotti³

et al. 2015

Journal of the American Society of Nephrology

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The critical role of genetic and epigenetic factors in the pathogenesis of kidney disorders is gradually becoming clear, and the need for disease models that recapitulate human kidney disorders in a personalized manner is paramount. In this study, we describe a method to select and amplify renal progenitor cultures from the urine of patients with kidney disorders. Urine-derived human renal progenitors exhibited phenotype and functional properties identical to those purified from kidney tissue, including the capacity to differentiate into tubular cells and podocytes, as demonstrated by confocal microscopy, Western blot analysis of podocyte-specific proteins, and scanning electron microscopy. Lineage tracing studies performed with conditional transgenic mice, in which podocytes are irreversibly tagged upon tamoxifen treatment (NPHS2.iCreER;mT/mG), that were subjected to doxorubicin nephropathy demonstrated that renal progenitors are the only urinary cell population that can be amplified in longterm culture. To validate the use of these cells for personalized modeling of kidney disorders, renal progenitors were obtained from (1) the urine of children with nephrotic syndrome and carrying potentially pathogenic mutations in genes encoding for podocyte proteins and (2) the urine of children without genetic alterations, as validated by next-generation sequencing. Renal progenitors obtained from patients carrying pathogenic mutations generated podocytes that exhibited an abnormal cytoskeleton structure and functional abnormalities compared with those obtained from patients with proteinuria but without genetic mutations. The results of this study demonstrate that urine-derived patient-specific renal progenitor cultures may be an innovative research tool for modeling of genetic kidney disorders. 26: 196126: -197426: , 201526: . doi: 10.1681 The incidence of AKI and CKD is rising and reaching epidemic proportions. 1 In patients with CKD, the progressive decline in renal function is multifactorial and attributable to a variety of mechanisms. 2 In particular, the critical role of genetic factors in the etiology, pathogenesis, and progression of many renal disorders is gradually becoming clear, especially in children. 3 Indeed, the advent of high-throughput sequencing techniques has fostered the identification of novel causative genes and allows the continuous discovery of genetic variants of unknown clinical significance, often raising the problem of the functional testing of their pathogenic role. 4 However, emerging evidence suggests that influence of the J Am Soc Nephrol

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Absolute and relative accuracy of rapid urine tests for urinary tract infection in children: a meta-analysis

Cited by 157 publications

References 78 publications

Urinary tract infection in children: clinical aspects and utility of urine dipstick test

Urinary tract infection in children: clinical aspects and utility of urine dipstick test

Standardization of isothermal microcalorimetry in urinary tract infection detection by using artificial urine

Human Urine-Derived Renal Progenitors for Personalized Modeling of Genetic Kidney Disorders

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