Absence of strand-specific repair of cyclobutane pyrimidine dimers in active genes in Drosophila melanogaster Kc cells

Cock, J.G.R. de; Klink, E.C.; Lohman, P.H.M.; Eeken, J.C.J.

doi:10.1016/0921-8777(92)90055-8

Cited by 12 publications

(5 citation statements)

References 22 publications

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“…An intriguing finding is the presence of XAB2 in the complete Drosophila genome sequence, in view of the notion that this organism appears to lack CSA and CSB homologs (45). This is consistent with an early report that Drosophila embryonal cells lack detectable TCR (46). The absence of a TCR pathway in Drosophila supports the idea that XAB2 has additional functions beyond TCR and is consistent with a role in the transcription process itself as suggested by the microinjection experiments.…”

Section: Xab2supporting

confidence: 80%

XAB2, a Novel Tetratricopeptide Repeat Protein Involved in Transcription-coupled DNA Repair and Transcription

Nakatsu¹,

Asahina²,

Citterio³

et al. 2000

Journal of Biological Chemistry

129

134

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Nucleotide excision repair is a highly versatile DNA repair system responsible for elimination of a wide variety of lesions from the genome. It is comprised of two subpathways: transcription-coupled repair that accomplishes efficient removal of damage blocking transcription and global genome repair. Recently, the basic mechanism of global genome repair has emerged from biochemical studies. However, little is known about transcription-coupled repair in eukaryotes. Here we report the identification of a novel protein designated XAB2 (XPA-binding protein 2) that was identified by virtue of its ability to interact with XPA, a factor central to both nucleotide excision repair subpathways. The XAB2 protein of 855 amino acids consists mainly of 15 tetratricopeptide repeats. In addition to interacting with XPA, immunoprecipitation experiments demonstrated that a fraction of XAB2 is able to interact with the transcription-coupled repair-specific proteins CSA and CSB as well as RNA polymerase II. Furthermore, antibodies against XAB2 inhibited both transcriptioncoupled repair and transcription in vivo but not global genome repair when microinjected into living fibroblasts. These results indicate that XAB2 is a novel component involved in transcription-coupled repair and transcription.

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Section: Xab2supporting

confidence: 80%

XAB2, a Novel Tetratricopeptide Repeat Protein Involved in Transcription-coupled DNA Repair and Transcription

Nakatsu¹,

Asahina²,

Citterio³

et al. 2000

Journal of Biological Chemistry

129

134

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“…In many eukaryotes, the template strand of transcribed regions is repaired more rapidly than the rest of the genome through a process termed transcription-coupled NER (TC-NER). Biochemical studies failed to find evidence for TC-NER in Drosophila (De Cock et al 1992). Consistent with this finding, two specialized proteins that mediate TC-NER, ERCC6 and ERCC8, are both missing from Drosophila (Sekelsky et al 2000a).…”

Section: Nucleotide Excision Repairsupporting

confidence: 53%

DNA Repair inDrosophila: Mutagens, Models, and Missing Genes

2017

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The numerous processes that damage DNA are counterbalanced by a complex network of repair pathways that, collectively, can mend diverse types of damage. Insights into these pathways have come from studies in many different organisms, including Drosophila melanogaster. Indeed, the first ideas about chromosome and gene repair grew out of Drosophila research on the properties of mutations produced by ionizing radiation and mustard gas. Numerous methods have been developed to take advantage of Drosophila genetic tools to elucidate repair processes in whole animals, organs, tissues, and cells. These studies have led to the discovery of key DNA repair pathways, including synthesis-dependent strand annealing, and DNA polymerase theta-mediated end joining. Drosophila appear to utilize other major repair pathways as well, such as base excision repair, nucleotide excision repair, mismatch repair, and interstrand crosslink repair. In a surprising number of cases, however, DNA repair genes whose products play important roles in these pathways in other organisms are missing from the Drosophila genome, raising interesting questions for continued investigations.

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“…Early studies using UV as the DNA damaging agent and the traditional T4 endonuclease V digestion followed by Southern hybridization assay reported that cultured Drosophila cells did not perform TCR (166,167). The experimental data were in accord with the genetic finding that Drosophila lacked homologs of the CSA and CSB genes that are known to be essential for TCR in humans, mice, and Arabidopsis (19,20).…”

Section: Transcription-coupled Repair In Drosophilamentioning

confidence: 53%

Molecular Mechanisms of Transcription-Coupled Repair

Selby

Lindsey-Boltz

et al. 2023

Annu. Rev. Biochem.

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Transcription-coupled repair (TCR), discovered as the preferential nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers located in transcribed mammalian genes compared to those in nontranscribed regions of the genome, is defined as faster repair of the transcribed strand (TS) versus the nontranscribed strand in transcribed genes. The phenomenon, universal in model organisms including Escherichia coli, yeast, Arabidopsis, mice, and humans, involves a translocase that interacts with both RNA polymerase stalled at damage in the TS and nucleotide excision repair proteins to accelerate repair. Drosophila, a notable exception, exhibits TCR but lacks an obvious TCR translocase. Mutations inactivating TCR genes cause increased damage-induced mutagenesis in E. coli and severe neurological and UV sensitivity syndromes in humans. To date, only E. coli TCR has been reconstituted in vitro with purified proteins. Detailed investigations of TCR using genome-wide next-generation sequencing methods, cryo–electron microscopy, single-molecule analysis, and other approaches have revealed fascinating mechanisms. Expected final online publication date for the Annual Review of Biochemistry, Volume 92 is June 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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Absence of strand-specific repair of cyclobutane pyrimidine dimers in active genes in Drosophila melanogaster Kc cells

Cited by 12 publications

References 22 publications

XAB2, a Novel Tetratricopeptide Repeat Protein Involved in Transcription-coupled DNA Repair and Transcription

XAB2, a Novel Tetratricopeptide Repeat Protein Involved in Transcription-coupled DNA Repair and Transcription

DNA Repair inDrosophila: Mutagens, Models, and Missing Genes

Molecular Mechanisms of Transcription-Coupled Repair

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