Absence of significant genotoxicity in lymphocytes and urine from workers exposed to moderate levels of cobalt-containing dust: A cross-sectional study

Boeck, Marlies De; Lardau, S; Buchet, Jean‐Pierre; Kirsch‐Volders, Micheline; Lison, Dominique

doi:10.1002/1098-2280(2000)36:2<151::aid-em10>3.0.co;2-v

Cited by 59 publications

(4 citation statements)

References 24 publications

(25 reference statements)

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“…The urinary levels of 8OHdG determined in this study for Monday morning (0.34-7.21 μg/g creatinine; median 2.46 μg/g creatinine for non-smokers and 1.71-5.23 μg/g creatinine, median 3.36 μg/g creatinine for smokers) were in agreement with other studies reporting 8OHdG concentrations in urine for controls (non-exposed non-smokers) and analyzed by HPLC techniques (3.3-5.6 μg/g creatinine, median 3.7 μg/g creatinine - [22,49,51-54]). We observed that the concentration of the oxidative stress marker 8OHdG increased over the two consecutive days of shift in non-smoking bus workers.…”

Section: Discussionsupporting

confidence: 92%

Biomarkers of oxidative stress and its association with the urinary reducing capacity in bus maintenance workers

Sauvain

Setyan

Wild

et al. 2011

J Occup Med Toxicol

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BackgroundExposure to particles (PM) induces adverse health effects (cancer, cardiovascular and pulmonary diseases). A key-role in these adverse effects seems to be played by oxidative stress, which is an excess of reactive oxygen species relative to the amount of reducing species (including antioxidants), the first line of defense against reactive oxygen species. The aim of this study was to document the oxidative stress caused by exposure to respirable particles in vivo, and to test whether exposed workers presented changes in their urinary levels for reducing species.MethodsBus depot workers (n = 32) exposed to particles and pollutants (respirable PM4, organic and elemental carbon, particulate metal content, polycyclic aromatic hydrocarbons, NOx, O3) were surveyed over two consecutive days. We collected urine samples before and after each shift, and quantified an oxidative stress biomarker (8-hydroxy-2'-deoxyguanosine), the reducing capacity and a biomarker of PAH exposure (1-hydroxypyrene). We used a linear mixed model to test for associations between the oxidative stress status of the workers and their particle exposure as well as with their urinary level of reducing species.ResultsWorkers were exposed to low levels of respirable PM4 (range 25-71 μg/m3). However, urinary levels of 8-hydroxy-2'-deoxyguanosine increased significantly within each shift and between both days for non-smokers. The between-day increase was significantly correlated (p < 0.001) with the concentrations of organic carbon, NOx, and the particulate copper content. The within-shift increase in 8OHdG was highly correlated to an increase of the urinary reducing capacity (Spearman ρ = 0.59, p < 0.0001).ConclusionsThese findings confirm that exposure to components associated to respirable particulate matter causes a systemic oxidative stress, as measured with the urinary 8OHdG. The strong association observed between urinary 8OHdG with the reducing capacity is suggestive of protective or other mechanisms, including circadian effects. Additional investigations should be performed to understand these observations.

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Section: Discussionsupporting

confidence: 92%

Biomarkers of oxidative stress and its association with the urinary reducing capacity in bus maintenance workers

Sauvain

Setyan

Wild

et al. 2011

J Occup Med Toxicol

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“…Thirteen (15.9%) studies evaluated the exposure to formaldehyde in fibreboard plants [ 42 ], pathology anatomy laboratories [ 61 , 62 , 63 ], the plywood industry [ 74 , 84 ], a furniture manufacturing facility [ 91 ], melamine tableware manufacturing workshops [ 116 , 117 , 118 ], and in hairdressers; one of these directly reporting formaldehyde exposure and including a control group [ 92 ] and the other assessing exposure to hair dyes and waiving and straightening products that also have formaldehyde in their composition [ 67 ]. Eleven (13.4%) studies were performed on dust, specifically marble dust [ 45 ], silica dust [ 47 ], wood dust [ 48 ], coal [ 95 ] and coal together with traffic air pollution [ 105 ], cobalt dust and other metals [ 64 ], tobacco dust [ 75 ], graphene [ 52 , 94 ], and two referred as dust particles [ 82 , 107 ]. Twelve (14.6%) studies were based on coke-oven exposure [ 57 , 59 , 72 , 81 , 85 , 106 , 109 , 110 , 111 , 113 , 114 , 115 ]; this type of emission usually consists of complex mixtures of dust, vapours, and gases, which can include carcinogens such as cadmium and arsenic.…”

Section: Resultsmentioning

confidence: 99%

“…Significantly higher DNA damage levels, as evaluated by the comet assay, were observed in 66 of these studies (81.5%). The remaining studies (n = 15, 18.5%) did not show statically significant results, namely PAH exposure [ 39 , 54 , 55 ], coke-oven PAH exposure [ 106 , 110 , 111 , 114 ], smelting [ 60 ], dust [ 64 , 107 ], traffic air pollution [ 71 ], JP-8 jet fuel [ 79 ], diesel exhaust [ 97 ], bitumen [ 86 ], and tobacco dust [ 108 ]. The study from Cavallo [ 52 ], in six graphene workers and eleven controls, used three comet descriptors, reaching statistically significant results with % DNA in the tail but not by using the tail moment and length.…”

Section: Resultsmentioning

confidence: 99%

The Comet Assay as a Tool in Human Biomonitoring Studies of Environmental and Occupational Exposure to Chemicals—A Systematic Scoping Review

Ladeira,

Møller,

Giovannelli

et al. 2024

Toxics

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Biomonitoring of human populations exposed to chemical substances that can act as potential mutagens or carcinogens, may enable the detection of damage and early disease prevention. In recent years, the comet assay has become an important tool for assessing DNA damage, both in environmental and occupational exposure contexts. To evidence the role of the comet assay in human biomonitoring, we have analysed original research studies of environmental or occupational exposure that used the comet assay in their assessments, following the PRISMA-ScR method (preferred reporting items for systematic reviews and meta-analyses extension for scoping reviews). Groups of chemicals were designated according to a broad classification, and the results obtained from over 300 original studies (n = 123 on air pollutants, n = 14 on anaesthetics, n = 18 on antineoplastic drugs, n = 57 on heavy metals, n = 59 on pesticides, and n = 49 on solvents) showed overall higher values of DNA strand breaks in the exposed subjects in comparison with the unexposed. In summary, our systematic scoping review strengthens the relevance of the use of the comet assay in assessing DNA damage in human biomonitoring studies.

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“…Different studies have reported changes in the 8-oxodG levels in urine induced by various environmental factors: (i) ambient PM2.5 (0.26 nmol/kg in autumn, 0.22 nmol/kg in winter, 0.24 nmol/kg in spring and 0.21 nmol/kg in summer) [ 84 , 124 ], (ii) silica (1.99 ± 0.94 µmol/mol of creatinine in silicotics and 2.28 ± 0.92 µmol/mol of creatinine in exposed workers) [ 87 ], (iii) chromium (1149.5 ± 759.5 pmol/kg in exposed workers vs. 730.2 ± 377.6 pmol/kg in controls) [ 125 ], (iv) cobalt (1.52 ± 1.69 mol/mol creatinine in individuals exposed to Co and 1.63 ± 1.42 mol/mol creatinine in individuals exposed to hard metals vs. 1.46 ± 1.48 mol/mol creatinine in controls) [ 126 ], (v) cobalt and chromium from hip-replacement implants (1.15 µmol/mol of creatinine for implants: 3–4 years; 0.75 µmol/mol of creatinine for implants: 1–2 years) [ 127 ], (vi) PAHs (2.6 ± 0.8 g/g of creatinine at the start and 2.5 ± 0.8 g/g of creatinine at the end of the week in roofers exposed to asphalt fume, 2.5 ± 1.7 g/g of creatinine at the start and 3.0 ± 1.7 g/g of creatinine at the end of the week in roofers exposed to coal-tar vs. 3.3 ± 1.9 g/g of creatinine at the start and 3.5 ± 1.7 g/g of creatinine at the end of the week in controls [ 88 ]; in engine room personnel of ships, the results were 23.3 nmo/L for individuals with skin contaminated by oil and 18.7 nmol/L for individuals without contaminated skin vs. 18.0 nmol/L for control [ 128 ]), (vii) welding fumes (1.7 μg L −1 /g of creatinine before and 2.1 μg L −1 /g of creatinine after 3 h of exposure) [ 129 ], and (viii) carcinogenic metals including nickel and cadmium [ 130 ].…”

Section: Determination Of 8-oxog and 8-oxodg By Hplc–ecdmentioning

confidence: 99%

8-oxoguanine and 8-oxodeoxyguanosine Biomarkers of Oxidative DNA Damage: A Review on HPLC–ECD Determination

Chiorcea-Paquim

2022

Molecules

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Reactive oxygen species (ROS) are continuously produced in living cells due to metabolic and biochemical reactions and due to exposure to physical, chemical and biological agents. Excessive ROS cause oxidative stress and lead to oxidative DNA damage. Within ROS-mediated DNA lesions, 8-oxoguanine (8-oxoG) and its nucleotide 8-oxo-2′-deoxyguanosine (8-oxodG)—the guanine and deoxyguanosine oxidation products, respectively, are regarded as the most significant biomarkers for oxidative DNA damage. The quantification of 8-oxoG and 8-oxodG in urine, blood, tissue and saliva is essential, being employed to determine the overall effects of oxidative stress and to assess the risk, diagnose, and evaluate the treatment of autoimmune, inflammatory, neurodegenerative and cardiovascular diseases, diabetes, cancer and other age-related diseases. High-performance liquid chromatography with electrochemical detection (HPLC–ECD) is largely employed for 8-oxoG and 8-oxodG determination in biological samples due to its high selectivity and sensitivity, down to the femtomolar range. This review seeks to provide an exhaustive analysis of the most recent reports on the HPLC–ECD determination of 8-oxoG and 8-oxodG in cellular DNA and body fluids, which is relevant for health research.

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Absence of significant genotoxicity in lymphocytes and urine from workers exposed to moderate levels of cobalt-containing dust: A cross-sectional study

Cited by 59 publications

References 24 publications

Biomarkers of oxidative stress and its association with the urinary reducing capacity in bus maintenance workers

Biomarkers of oxidative stress and its association with the urinary reducing capacity in bus maintenance workers

The Comet Assay as a Tool in Human Biomonitoring Studies of Environmental and Occupational Exposure to Chemicals—A Systematic Scoping Review

8-oxoguanine and 8-oxodeoxyguanosine Biomarkers of Oxidative DNA Damage: A Review on HPLC–ECD Determination

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