Absence of quenching by [Fe(CN)6]4− is not proof of DNA intercalation

Burya, Scott J.; Lutterman, Daniel A.; Turró, Claudia

doi:10.1039/c0cc04973e

Cited by 21 publications

(15 citation statements)

References 43 publications

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“…x 10 4 L mol −1 , indicating the existence of an interaction with DNA that may be further explored as a molecular biology tool or in therapy, even though being smaller than classics DNA intercalators, such as [Ru(bpy) 2 (dppz)] 2+ (K b = 3.2 x 10 6 L mol −1 ) [31] and [Ru(phen) 2 (dppz)] 2+ (K b = 5.1 x 10 6 L mol −1 ). …”

Section: Dna Titration Monitored By Electronic Absorption Spectroscopymentioning

confidence: 99%

Ruthenium(II) bipyridine complexes with pendant anthracenyl and naphthyl moieties: A strategy for a ROS generator with DNA binding selectivity

Abreu

Diógenes

Lopes

et al. 2016

Inorganica Chimica Acta

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Section: Dna Titration Monitored By Electronic Absorption Spectroscopymentioning

confidence: 99%

Ruthenium(II) bipyridine complexes with pendant anthracenyl and naphthyl moieties: A strategy for a ROS generator with DNA binding selectivity

Abreu

Diógenes

Lopes

et al. 2016

Inorganica Chimica Acta

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“…We also observe small fluorescence quenching for the complexes without CT-DNA, which indicated that DNA protects complexes from quenchers. Although fluorescence quenching experiment by ferrocyanide cannot be used to prove that DNA protects complexes by intercalation, it can determine DNA affinity of the complex (Burya, Lutterman, & Turro, 2011). Here, the higher quenching efficiency for complex 1 with DNA indicated that it shows lower DNA affinity than complex 2.…”

Section: Emission Titration Experimentsmentioning

confidence: 90%

DNA photocleavage, topoisomerase I inhibition, and cytotoxicities of two ruthenium complexes containing asymmetry ligand

Liu

Deng

et al. 2020

Journal of Radiation Research and Applied Sciences

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In recent years, ruthenium (II) complexes have attracted attention due to high DNA topoisomerase I inhibitory activities and good anticancer activities. Herein, an asymmetric ligand 2-(5-(1, 10-phenanthroline))-1 H-4,5-diphenyl-imidazole (pdpi) and its ruthenium complexes were synthesized. DNA-binding mode of two complexes was determined as intercalation by using UV-vis spectra, emission spectra, viscosity, and molecular docking experiments. DNA photocleavage experimental results show that two ruthenium complexes effectively cleave plasmid DNA by producing singlet oxygen. Furthermore, they both displayed good topo I inhibition activities. The cytotoxic activities results show that complex 2 displays better antitumor activities against Eca-109 cells and A549 cells (IC 50 = 25.97 ± 3.33 μM and 28.83 ± 2.49 μM, respectively) compared to complex 1.

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“…However, it is noteworthy that the absence of quenching by [Fe(CN) 6 ] 4− is not a proof of DNA intercalation. [39] As illustrated in Fig. S1, in .…”

Section: Luminescence Quenching By [Fe(cn) 6 ] 4−mentioning

confidence: 93%

A Promising Dna Groove Binder and Photocleaver Based on a Dinuclear Ruthenium(ii) Complex

Wang

et al. 2019

J. Chil. Chem. Soc.

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A dinuclear ruthenium complex [Ru 2 (bpy) 4 (bip-phenol)](ClO 4) 4 {bpy = 2,2′-bipyridine, bip-phenol = 2,4-bis(1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl) phenol} has been synthesized and characterized. The calf thymus (ct) DNA binding properties of the complex are investigated by means of DNA viscosity and optical spectroscopic techniques of UV-visible absorption and emission spectral titrations, steady-state emission quenching with ferrocyanide, ethidium bromide competitive binding, DNA thermal denaturation and reverse salt effect, together with molecular simulation technology. The results suggest that the complex is a promising DNA groove binder with a large DNA binding constant on 10 6 M −1 order of magnitude. The fluorescence of the complex manifests by 6.3-fold upon binding saturately to DNA. The complex is also demonstrated to be an efficient photocleaver of pBR 322 DNA.

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Absence of quenching by [Fe(CN)6]4− is not proof of DNA intercalation

Cited by 21 publications

References 43 publications

Ruthenium(II) bipyridine complexes with pendant anthracenyl and naphthyl moieties: A strategy for a ROS generator with DNA binding selectivity

Ruthenium(II) bipyridine complexes with pendant anthracenyl and naphthyl moieties: A strategy for a ROS generator with DNA binding selectivity

DNA photocleavage, topoisomerase I inhibition, and cytotoxicities of two ruthenium complexes containing asymmetry ligand

A Promising Dna Groove Binder and Photocleaver Based on a Dinuclear Ruthenium(ii) Complex

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