Absence of molecular evidence of Leptospira spp. in urine samples collected from rodents captured in Yucatán, México

Abstract

ABSTRACT. Leptospira spp. is a spirochete bacteria, causal agent of leptospirosis, zoonotic disease endemic in México that represents a serious public health and veterinary problem. Rodents are recognised as the most important reservoirs of this bacteria, which is transmitted mainly through direct or indirect contact with the Leptospira spp. excreted in the urine of infected individuals. The aim of this study was to evaluate the circulation of Leptospira spp. in urine samples of wild and synanthropic rodents from Yucatán, México. Eighty-four rodents were captured in the community of Cenotillo, Yucatán. Twenty-six urine samples were collected from the bladder and were used in the total DNA extraction. The identification of Leptospira spp. was intended through the polymerase chain reaction test in its endpoint variant. No evidence of Leptospira spp. was found in the urine samples. It is necessary to use other tissues for the identification of Leptospira spp., before concluding that the rodents used in the present study are not reservoirs of this bacteria.

A survey of zoonotic pathogens carried by house mouse and black rat populations in Yucatan, Mexico

“…The house mouse and the black rat are a threat to public health; however, few studies have evaluated their role as carriers of zoonotic pathogens in urban and rural settlements of Mexico [31, 34, 43, 44]. In this study, we report the presence of T. cruzi, H. diminuta and L. interrogans among house mouse and black rat populations from two urban neighbourhoods and a rural village from Yucatan, Mexico.…”

“…Epidemiologic studies of human leptospirosis have reported seroprevalences of ~14%, with the icterohaemorrhagiae serovar predominant in the icteric cases [29, 30]. In rodents, L. interrogans serovar icterohaemorrhagiae has been reported as the predominant serovar [30, 31]. In Yucatecan children, H. nana is a common cestode [32, 33], whereas H. diminuta has not been reported.…”

Histological lesions associated to the possible infection by Trypanosoma cruzi (Chagas, 1909) in hearts of synanthropic rodents captured in Yucatan, Mexico

“…100X lens.DiscusiónEstudios previos conducidos con roedores sinantrópicos capturados en Yucatán, han demostrado que estos animales son reservorios o portadores de agentes zoonóticos, tales como Leptospira spp.Noguchi 1917 emend. Faine and Stallman 1982(Torres-Castro et al 2014, Rickettsia felis Bouyer…”

Estudio sobre roedores sinántropicos como reservorios de patógenos zoonóticos en Yucatán

“…Leptospira interrogans y Leptospira kirschneri, son dos de las especies patógenas pertenecientes al género Leptospira que pueden ocasionar leptospirosis, la cual es presumiblemente la enfermedad zoonótica más diseminada a nivel mundial, endémica en la mayor parte del territorio nacional (11). Estas especies fueron localizadas por medio de técnicas moleculares en tejido renal de R. rattus y M. musculus capturados en una comunidad rural del sur de Yucatán (12,13). Además, Vado-Solís y colaboradores (14), reportaron 15% de seropositividad para las serovares icterohaemorrhagiae, wolffi y bratislava, incluidas en la especie Leptospira interrogans, en 60 roedores capturados en varias granjas y ranchos de Yucatán.…”